Potential Properties of Plant Sprout Extracts on Amyloid β
The aim of this study is to examine the amyloid β (Aβ) inhibition mechanism of plant sprouts’ aqueous extracts (PSAE). In this study, we screened the effects of five plant sprouts’ extracts on Aβ (1–42) structure modification using gel electrophoresis. In PSAE, no band of Aβ monomer was recognized i...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2016/9347468 |
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| author | Mizue Okada Yoshinori Okada |
| author_facet | Mizue Okada Yoshinori Okada |
| author_sort | Mizue Okada |
| collection | DOAJ |
| description | The aim of this study is to examine the amyloid β (Aβ) inhibition mechanism of plant sprouts’ aqueous extracts (PSAE). In this study, we screened the effects of five plant sprouts’ extracts on Aβ (1–42) structure modification using gel electrophoresis. In PSAE, no band of Aβ monomer was recognized in Japanese butterbur. Similarly, the Aβ monomer band became light in buckwheat, red cabbage, broccoli, and brussels. The neuroprotective effects of PSAE were evaluated by measuring levels of Aβ in mixtures (Aβ and PSAE) with Aβ ELISA assay. The treatment with PSAE decreased Aβ levels. The results indicated that the levels of red cabbage, Japanese butterbur, and broccoli were 9.6, 28.0, and 44.0%, respectively. The lowest value was observed with buckwheat. Furthermore, we carried out a Congo Red (CR) and Aβ binding experiment of PSAE to confirm the modification mechanism of PSAE. The correlation coefficient for the absorption spectrum peak of CR was found to be bigger than 0.8 (r=0.882) which proved that the Aβ levels could be attributed to the peak of CR. In conclusion, we demonstrated that treatment with PSAE effectively decreases Aβ concentration. Thus, the mechanism that decreased the Aβ levels may be modification by PSAE. |
| format | Article |
| id | doaj-art-3ea1ddd74c9c4c0d80e9cb5c22022746 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Biochemistry Research International |
| spelling | doaj-art-3ea1ddd74c9c4c0d80e9cb5c220227462025-02-03T01:21:25ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/93474689347468Potential Properties of Plant Sprout Extracts on Amyloid βMizue Okada0Yoshinori Okada1Nutrition Section, Ageing and Nutrition Research, Yms Laboratory, Gifu 503-1306, JapanLaboratory on Ageing & Health Management, Graduate School of Nursing & Health, Aichi Prefectural University, Tohgoku, Kamishidami, Moriyama, Nagoya 463-8502, JapanThe aim of this study is to examine the amyloid β (Aβ) inhibition mechanism of plant sprouts’ aqueous extracts (PSAE). In this study, we screened the effects of five plant sprouts’ extracts on Aβ (1–42) structure modification using gel electrophoresis. In PSAE, no band of Aβ monomer was recognized in Japanese butterbur. Similarly, the Aβ monomer band became light in buckwheat, red cabbage, broccoli, and brussels. The neuroprotective effects of PSAE were evaluated by measuring levels of Aβ in mixtures (Aβ and PSAE) with Aβ ELISA assay. The treatment with PSAE decreased Aβ levels. The results indicated that the levels of red cabbage, Japanese butterbur, and broccoli were 9.6, 28.0, and 44.0%, respectively. The lowest value was observed with buckwheat. Furthermore, we carried out a Congo Red (CR) and Aβ binding experiment of PSAE to confirm the modification mechanism of PSAE. The correlation coefficient for the absorption spectrum peak of CR was found to be bigger than 0.8 (r=0.882) which proved that the Aβ levels could be attributed to the peak of CR. In conclusion, we demonstrated that treatment with PSAE effectively decreases Aβ concentration. Thus, the mechanism that decreased the Aβ levels may be modification by PSAE.http://dx.doi.org/10.1155/2016/9347468 |
| spellingShingle | Mizue Okada Yoshinori Okada Potential Properties of Plant Sprout Extracts on Amyloid β Biochemistry Research International |
| title | Potential Properties of Plant Sprout Extracts on Amyloid β |
| title_full | Potential Properties of Plant Sprout Extracts on Amyloid β |
| title_fullStr | Potential Properties of Plant Sprout Extracts on Amyloid β |
| title_full_unstemmed | Potential Properties of Plant Sprout Extracts on Amyloid β |
| title_short | Potential Properties of Plant Sprout Extracts on Amyloid β |
| title_sort | potential properties of plant sprout extracts on amyloid β |
| url | http://dx.doi.org/10.1155/2016/9347468 |
| work_keys_str_mv | AT mizueokada potentialpropertiesofplantsproutextractsonamyloidb AT yoshinoriokada potentialpropertiesofplantsproutextractsonamyloidb |