A one-pot method for universal Dengue virus detection by combining RT-RPA amplification and CRISPR/Cas12a assay

A one-pot method for universal Dengue virus detection by combining RT-RPA amplification and CRISPR/Cas12a assay

Abstract Dengue Virus (DENV) is a life-threatening pathogen leading to dengue fever, which brings about huge public health challenges globally. However, traditional detection methods currently fail to meet the increasing demands of clinic practice in terms of speed, simplicity, and accuracy. To addr...

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Bibliographic Details
Main Authors: Yunkai Zhang, Yan Xiang, Dengyong Hou, Liben Fang, Shuqi Cai, Jianping Zhang, Yujia Wang, Yuyu Jiang, Bin Liu, Jie Bai, Yue Ding, Jingjing Fang, Shuanghong Chen, Xingguang Liu, Xiaomeng Ren
Format: Article
Language:English
Published: BMC 2025-03-01
Series:BMC Microbiology
Subjects:
DENV
RPA
CRISPR/Cas12a
One-pot
LFD
Universal detection
Online Access:https://doi.org/10.1186/s12866-025-03882-z
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