<i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica
The psychrophilic aerobic heterotrophic bacterium, strain 1639<sup>T</sup>, was isolated from the low-temperature Lake Untersee in Antarctica. The bacterium was Gram-positive, non-motile, yellow–green-pigmented, non-spore-forming, and a pleomorphic rod. Growth was observed at temperature...
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2025-04-01
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| author | Yulia Yu. Berestovskaya Tatyana P. Tourova Denis S. Grouzdev Natalyia V. Potekhina Dmitry S. Kopitsyn Nikolay V. Pimenov Lina V. Vasilyeva |
| author_facet | Yulia Yu. Berestovskaya Tatyana P. Tourova Denis S. Grouzdev Natalyia V. Potekhina Dmitry S. Kopitsyn Nikolay V. Pimenov Lina V. Vasilyeva |
| author_sort | Yulia Yu. Berestovskaya |
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| description | The psychrophilic aerobic heterotrophic bacterium, strain 1639<sup>T</sup>, was isolated from the low-temperature Lake Untersee in Antarctica. The bacterium was Gram-positive, non-motile, yellow–green-pigmented, non-spore-forming, and a pleomorphic rod. Growth was observed at temperatures of 0–25 °C with an optimum at 10 °C. The strain used urea as a nitrogen source. The major fatty acids were i-C<sub>16:0</sub> (49.69%), ai-C<sub>15:0</sub> (17.59%), and C<sub>16:1</sub> branched (12.03%). Identified polar lipids were phosphatidylglycerols and a glycolipid. The respiratory quinone was determined to be MK-10. The genomic DNA G+C content was 68.03 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1639<sup>T</sup> was a member of the genus <i>Cryobacterium</i>, with the highest sequence similarity to <i>C. arcticum</i> SK1<sup>T</sup> (98.4%), <i>C. soli</i> GCJ02<sup>T</sup> (98.4%), <i>C. lactosi</i> Sr59<sup>T</sup> (98.3%), <i>C. zongtaii</i> TMN-42<sup>T</sup> (98.2%), and <i>C. adonitolivorans</i> RHLS22-1<sup>T</sup> (98.1%). The ANI and the DNA–DNA hybridization estimate values between strain 1639<sup>T</sup> and all type strains of species of the genus <i>Cryobacterium</i> were in the range of 84.3–87.8% and 20.5–40.3%, respectively. The combined genotypic and phenotypic data indicate that strain 1639<sup>T</sup> represents a novel species within the genus <i>Cryobacterium</i>, for which the name <i>Cryobacterium inferilacus</i> sp. nov. is proposed with the type strain 1639<sup>T</sup> (=KCTC 59142<sup>T</sup>, =VKM Ac-2907<sup>T</sup>, UQM 41460<sup>T</sup>). |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-3e9c71ee443344d8acf28ba6868e53822025-08-20T03:48:02ZengMDPI AGMicroorganisms2076-26072025-04-0113599010.3390/microorganisms13050990<i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in AntarcticaYulia Yu. Berestovskaya0Tatyana P. Tourova1Denis S. Grouzdev2Natalyia V. Potekhina3Dmitry S. Kopitsyn4Nikolay V. Pimenov5Lina V. Vasilyeva6Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, RussiaSciBear OU, 10115 Tallinn, EstoniaFaculty of Biology, Lomonosov Moscow State University, Moscow 119234, RussiaDepartment of Physical and Colloid Chemistry, Faculty of Chemical and Environmental Engineering, Gubkin University, 65-1, Moscow 119991, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, RussiaThe psychrophilic aerobic heterotrophic bacterium, strain 1639<sup>T</sup>, was isolated from the low-temperature Lake Untersee in Antarctica. The bacterium was Gram-positive, non-motile, yellow–green-pigmented, non-spore-forming, and a pleomorphic rod. Growth was observed at temperatures of 0–25 °C with an optimum at 10 °C. The strain used urea as a nitrogen source. The major fatty acids were i-C<sub>16:0</sub> (49.69%), ai-C<sub>15:0</sub> (17.59%), and C<sub>16:1</sub> branched (12.03%). Identified polar lipids were phosphatidylglycerols and a glycolipid. The respiratory quinone was determined to be MK-10. The genomic DNA G+C content was 68.03 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1639<sup>T</sup> was a member of the genus <i>Cryobacterium</i>, with the highest sequence similarity to <i>C. arcticum</i> SK1<sup>T</sup> (98.4%), <i>C. soli</i> GCJ02<sup>T</sup> (98.4%), <i>C. lactosi</i> Sr59<sup>T</sup> (98.3%), <i>C. zongtaii</i> TMN-42<sup>T</sup> (98.2%), and <i>C. adonitolivorans</i> RHLS22-1<sup>T</sup> (98.1%). The ANI and the DNA–DNA hybridization estimate values between strain 1639<sup>T</sup> and all type strains of species of the genus <i>Cryobacterium</i> were in the range of 84.3–87.8% and 20.5–40.3%, respectively. The combined genotypic and phenotypic data indicate that strain 1639<sup>T</sup> represents a novel species within the genus <i>Cryobacterium</i>, for which the name <i>Cryobacterium inferilacus</i> sp. nov. is proposed with the type strain 1639<sup>T</sup> (=KCTC 59142<sup>T</sup>, =VKM Ac-2907<sup>T</sup>, UQM 41460<sup>T</sup>).https://www.mdpi.com/2076-2607/13/5/990psychrophilic bacteriumlow-temperature ecosystem<i>Cryobacterium</i>family <i>Microbacteriaceae</i>Antarcticaactinomycetes |
| spellingShingle | Yulia Yu. Berestovskaya Tatyana P. Tourova Denis S. Grouzdev Natalyia V. Potekhina Dmitry S. Kopitsyn Nikolay V. Pimenov Lina V. Vasilyeva <i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica Microorganisms psychrophilic bacterium low-temperature ecosystem <i>Cryobacterium</i> family <i>Microbacteriaceae</i> Antarctica actinomycetes |
| title | <i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica |
| title_full | <i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica |
| title_fullStr | <i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica |
| title_full_unstemmed | <i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica |
| title_short | <i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica |
| title_sort | i cryobacterium inferilacus i sp nov a pshychrophilic ureolitic bacterium from lake untersee in antarctica |
| topic | psychrophilic bacterium low-temperature ecosystem <i>Cryobacterium</i> family <i>Microbacteriaceae</i> Antarctica actinomycetes |
| url | https://www.mdpi.com/2076-2607/13/5/990 |
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