<i>Cryobacterium Inferilacus</i> sp. nov., a Pshychrophilic Ureolitic Bacterium From Lake Untersee in Antarctica
The psychrophilic aerobic heterotrophic bacterium, strain 1639<sup>T</sup>, was isolated from the low-temperature Lake Untersee in Antarctica. The bacterium was Gram-positive, non-motile, yellow–green-pigmented, non-spore-forming, and a pleomorphic rod. Growth was observed at temperature...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/5/990 |
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| Summary: | The psychrophilic aerobic heterotrophic bacterium, strain 1639<sup>T</sup>, was isolated from the low-temperature Lake Untersee in Antarctica. The bacterium was Gram-positive, non-motile, yellow–green-pigmented, non-spore-forming, and a pleomorphic rod. Growth was observed at temperatures of 0–25 °C with an optimum at 10 °C. The strain used urea as a nitrogen source. The major fatty acids were i-C<sub>16:0</sub> (49.69%), ai-C<sub>15:0</sub> (17.59%), and C<sub>16:1</sub> branched (12.03%). Identified polar lipids were phosphatidylglycerols and a glycolipid. The respiratory quinone was determined to be MK-10. The genomic DNA G+C content was 68.03 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1639<sup>T</sup> was a member of the genus <i>Cryobacterium</i>, with the highest sequence similarity to <i>C. arcticum</i> SK1<sup>T</sup> (98.4%), <i>C. soli</i> GCJ02<sup>T</sup> (98.4%), <i>C. lactosi</i> Sr59<sup>T</sup> (98.3%), <i>C. zongtaii</i> TMN-42<sup>T</sup> (98.2%), and <i>C. adonitolivorans</i> RHLS22-1<sup>T</sup> (98.1%). The ANI and the DNA–DNA hybridization estimate values between strain 1639<sup>T</sup> and all type strains of species of the genus <i>Cryobacterium</i> were in the range of 84.3–87.8% and 20.5–40.3%, respectively. The combined genotypic and phenotypic data indicate that strain 1639<sup>T</sup> represents a novel species within the genus <i>Cryobacterium</i>, for which the name <i>Cryobacterium inferilacus</i> sp. nov. is proposed with the type strain 1639<sup>T</sup> (=KCTC 59142<sup>T</sup>, =VKM Ac-2907<sup>T</sup>, UQM 41460<sup>T</sup>). |
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| ISSN: | 2076-2607 |