Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western India
Background: Microbiological diagnosis of pediatric pulmonary and intestinal tuberculosis remains difficult due to difficulty in specimen collection such as with sputum, gastric aspirates or intestinal biopsies. Stool Xpert MTB/Rif may be useful as specimen collection is non-invasive. Objectives: To...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-03-01
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| Series: | Lung India |
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| Online Access: | https://journals.lww.com/10.4103/lungindia.lungindia_528_24 |
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| author | Ira Shah Dhruv N. Gandhi Dhruv Mamtora Meenakshi Dey Suhani Jain Sonal Patil Maneesha D’souza Sanjay Mattoo Daksha Shah Varsha Puri Minnie Bodhanwala |
| author_facet | Ira Shah Dhruv N. Gandhi Dhruv Mamtora Meenakshi Dey Suhani Jain Sonal Patil Maneesha D’souza Sanjay Mattoo Daksha Shah Varsha Puri Minnie Bodhanwala |
| author_sort | Ira Shah |
| collection | DOAJ |
| description | Background:
Microbiological diagnosis of pediatric pulmonary and intestinal tuberculosis remains difficult due to difficulty in specimen collection such as with sputum, gastric aspirates or intestinal biopsies. Stool Xpert MTB/Rif may be useful as specimen collection is non-invasive.
Objectives:
To determine the sensitivity and specificity of Stool Xpert for the diagnosis of pulmonary or intestinal tuberculosis in Indian children.
Methods:
We retrospectively compared the performance of stool Xpert, pulmonary sample Xpert (gastric lavage, sputum, bronchoalveolar lavage), and Mycobacteria Growth Indicator Tube (MGIT) for 52 children with pulmonary, intestinal or disseminated tuberculosis.
Results:
Thirty (57.7%) children had positive pulmonary Xpert results, while 17(32.7%) had positive stool Xpert results. The sensitivity and specificity of stool Xpert compared to pulmonary Xpert was 43.3% and 81.8%, respectively. On using MGIT as a reference, pulmonary Xpert had a higher sensitivity compared to stool Xpert (81.8% vs. 63.6%, respectively) but lower specificity (48.8%vs.75.6%, respectively). Kappa analysis showed fair agreement between stool and pulmonary Xpert results (0.23), and moderate agreement between stool and gastric lavage Xpert results (0.43). Stool Xpert was found to be equivalent to pulmonary Xpert in detecting tuberculosis in children younger than 5 years (36.4% in both; 4 children each tested positive out of 11). No patients with isolated intestinal tuberculosis had positive stool Xpert.
Conclusion:
Stool Xpert may serve as a supplementary diagnostic tool in paediatric tuberculosis, particularly in young children where specimen collection is difficult. However, its lower sensitivity compared to pulmonary samples limits its potential as a standalone test. |
| format | Article |
| id | doaj-art-3e9be36009fb4094a620cbbdf67b7533 |
| institution | DOAJ |
| issn | 0970-2113 0974-598X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Lung India |
| spelling | doaj-art-3e9be36009fb4094a620cbbdf67b75332025-08-20T02:52:47ZengWolters Kluwer Medknow PublicationsLung India0970-21130974-598X2025-03-0142211511910.4103/lungindia.lungindia_528_24Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western IndiaIra ShahDhruv N. GandhiDhruv MamtoraMeenakshi DeySuhani JainSonal PatilManeesha D’souzaSanjay MattooDaksha ShahVarsha PuriMinnie BodhanwalaBackground: Microbiological diagnosis of pediatric pulmonary and intestinal tuberculosis remains difficult due to difficulty in specimen collection such as with sputum, gastric aspirates or intestinal biopsies. Stool Xpert MTB/Rif may be useful as specimen collection is non-invasive. Objectives: To determine the sensitivity and specificity of Stool Xpert for the diagnosis of pulmonary or intestinal tuberculosis in Indian children. Methods: We retrospectively compared the performance of stool Xpert, pulmonary sample Xpert (gastric lavage, sputum, bronchoalveolar lavage), and Mycobacteria Growth Indicator Tube (MGIT) for 52 children with pulmonary, intestinal or disseminated tuberculosis. Results: Thirty (57.7%) children had positive pulmonary Xpert results, while 17(32.7%) had positive stool Xpert results. The sensitivity and specificity of stool Xpert compared to pulmonary Xpert was 43.3% and 81.8%, respectively. On using MGIT as a reference, pulmonary Xpert had a higher sensitivity compared to stool Xpert (81.8% vs. 63.6%, respectively) but lower specificity (48.8%vs.75.6%, respectively). Kappa analysis showed fair agreement between stool and pulmonary Xpert results (0.23), and moderate agreement between stool and gastric lavage Xpert results (0.43). Stool Xpert was found to be equivalent to pulmonary Xpert in detecting tuberculosis in children younger than 5 years (36.4% in both; 4 children each tested positive out of 11). No patients with isolated intestinal tuberculosis had positive stool Xpert. Conclusion: Stool Xpert may serve as a supplementary diagnostic tool in paediatric tuberculosis, particularly in young children where specimen collection is difficult. However, its lower sensitivity compared to pulmonary samples limits its potential as a standalone test.https://journals.lww.com/10.4103/lungindia.lungindia_528_24childhood tbdrug-resistant tbdrug-sensitive tbgenexpertintestinal tbpediatric tbstool genexperttb |
| spellingShingle | Ira Shah Dhruv N. Gandhi Dhruv Mamtora Meenakshi Dey Suhani Jain Sonal Patil Maneesha D’souza Sanjay Mattoo Daksha Shah Varsha Puri Minnie Bodhanwala Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western India Lung India childhood tb drug-resistant tb drug-sensitive tb genexpert intestinal tb pediatric tb stool genexpert tb |
| title | Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western India |
| title_full | Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western India |
| title_fullStr | Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western India |
| title_full_unstemmed | Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western India |
| title_short | Utility of stool Xpert MTB/Rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children - A study from Western India |
| title_sort | utility of stool xpert mtb rif assay in the diagnosis of pulmonary and abdominal tuberculosis in children a study from western india |
| topic | childhood tb drug-resistant tb drug-sensitive tb genexpert intestinal tb pediatric tb stool genexpert tb |
| url | https://journals.lww.com/10.4103/lungindia.lungindia_528_24 |
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