Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM
The biofabricated silver nanoparticles are extensively used in environmental, biotechnological and biomedical applications. The synthesis of SNPs has been carried out by using the filtrate extract of novel fungal strain Penicillium atramentosum KM. To undertake this study, P. atramentosum KM extract...
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| Format: | Article |
| Language: | English |
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Springer
2015-11-01
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| Series: | Journal of Saudi Chemical Society |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319610314000921 |
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| author | Vikas Sarsar Manjit K. Selwal Krishan K. Selwal |
| author_facet | Vikas Sarsar Manjit K. Selwal Krishan K. Selwal |
| author_sort | Vikas Sarsar |
| collection | DOAJ |
| description | The biofabricated silver nanoparticles are extensively used in environmental, biotechnological and biomedical applications. The synthesis of SNPs has been carried out by using the filtrate extract of novel fungal strain Penicillium atramentosum KM. To undertake this study, P. atramentosum KM extract was exposed to silver nitrate and the obtained SNPs were thoroughly analyzed using physicochemical characterization tools such as UV–visible spectroscopy (UV–vis), Fourier transformation infrared (FTIR), X-ray diffraction (XRD) and transmission electron microscopy (TEM). As evident from the FTIR spectra plausibly the protein components of fungal extract caused the reduction of silver nitrate. The SNPs showed a characteristic UV–visible peak at 420 nm with an average size of 5–25 nm. The XRD record exhibited the characteristic peaks of 111, 200, 220 and 311 nanoparticles signifying that these nanoparticles were crystalline in nature. Parametric optimization showed maximum absorbance of 420 nm at pH 7, 25 °C with 3 mM silver nitrate, concentration ratio of fungal extract and silver nitrate was 5:5 in 72 h. The synthesized SNPs showed antimicrobial activity against bacterial strains. |
| format | Article |
| id | doaj-art-3e958da4172c451da7de50c6bcd601d1 |
| institution | Kabale University |
| issn | 1319-6103 |
| language | English |
| publishDate | 2015-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Saudi Chemical Society |
| spelling | doaj-art-3e958da4172c451da7de50c6bcd601d12025-08-20T03:48:41ZengSpringerJournal of Saudi Chemical Society1319-61032015-11-0119668268810.1016/j.jscs.2014.07.001Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KMVikas SarsarManjit K. SelwalKrishan K. SelwalThe biofabricated silver nanoparticles are extensively used in environmental, biotechnological and biomedical applications. The synthesis of SNPs has been carried out by using the filtrate extract of novel fungal strain Penicillium atramentosum KM. To undertake this study, P. atramentosum KM extract was exposed to silver nitrate and the obtained SNPs were thoroughly analyzed using physicochemical characterization tools such as UV–visible spectroscopy (UV–vis), Fourier transformation infrared (FTIR), X-ray diffraction (XRD) and transmission electron microscopy (TEM). As evident from the FTIR spectra plausibly the protein components of fungal extract caused the reduction of silver nitrate. The SNPs showed a characteristic UV–visible peak at 420 nm with an average size of 5–25 nm. The XRD record exhibited the characteristic peaks of 111, 200, 220 and 311 nanoparticles signifying that these nanoparticles were crystalline in nature. Parametric optimization showed maximum absorbance of 420 nm at pH 7, 25 °C with 3 mM silver nitrate, concentration ratio of fungal extract and silver nitrate was 5:5 in 72 h. The synthesized SNPs showed antimicrobial activity against bacterial strains.http://www.sciencedirect.com/science/article/pii/S1319610314000921Antimicrobial activityFTIRSilver nanoparticlesTEMUV–vis spectroscopyXRD |
| spellingShingle | Vikas Sarsar Manjit K. Selwal Krishan K. Selwal Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM Journal of Saudi Chemical Society Antimicrobial activity FTIR Silver nanoparticles TEM UV–vis spectroscopy XRD |
| title | Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM |
| title_full | Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM |
| title_fullStr | Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM |
| title_full_unstemmed | Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM |
| title_short | Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM |
| title_sort | biofabrication characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of penicillium atramentosum km |
| topic | Antimicrobial activity FTIR Silver nanoparticles TEM UV–vis spectroscopy XRD |
| url | http://www.sciencedirect.com/science/article/pii/S1319610314000921 |
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