Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea

Abstract Oxygen-regulated protein 150 (ORP150) is a chaperone found in the endoplasmic reticulum (ER) induced by ER stress, oxidative stress, glutamate toxicity, ischemia, and hypoxia. Increased expression of ORP150 protects the cells by stopping ER stress, maintaining calcium balance, and delaying...

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Main Authors: Önder Otlu, Mehmet Erdem, Tuğba Raika Kıran, Feyza İnceoğlu, Ayşegül Altıntop Geçkil, Nurcan Kırıcı Berber, Erdal İn
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-99987-6
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author Önder Otlu
Mehmet Erdem
Tuğba Raika Kıran
Feyza İnceoğlu
Ayşegül Altıntop Geçkil
Nurcan Kırıcı Berber
Erdal İn
author_facet Önder Otlu
Mehmet Erdem
Tuğba Raika Kıran
Feyza İnceoğlu
Ayşegül Altıntop Geçkil
Nurcan Kırıcı Berber
Erdal İn
author_sort Önder Otlu
collection DOAJ
description Abstract Oxygen-regulated protein 150 (ORP150) is a chaperone found in the endoplasmic reticulum (ER) induced by ER stress, oxidative stress, glutamate toxicity, ischemia, and hypoxia. Increased expression of ORP150 protects the cells by stopping ER stress, maintaining calcium balance, and delaying apoptosis. In this study, we aim to investigate serum ORP150 amount in patients with different severity levels of obstructive sleep apnea (OSA). Forty-nine patients (25 of severe and 24 of mild-moderate), and 23 healthy controls were included in the study. Routine biochemical measurements and serum ORP150 measurements of all groups and sleep quality measurements of OSA groups were obtained. ELISA was used to measure ORP150 levels in serum samples. In addition, ROC analysis was performed to determine the diagnostic power of the ORP150 parameter. There are significant differences between all three groups in terms of ORP150 values (severe OSA: 8.03 ± 0.4 ng/mL; mild-moderate OSA: 5.54 ± 0.47 ng/mL; control: 4.41 ± 0.25 ng/mL, p < 0.017). The highest ORP150 level belongs to the severe OSA group and there is a direct correlation between the severity of the disease and ORP150 levels. ORP150 value is a distinguishing parameter for OSA and the cut-off value of ORP150 was observed as 7.14 ng/mL. We concluded that serum ORP150 levels can be a differential diagnostic parameter in OSA patients and that disease severity can be determined by serum ORP150 measurement.
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spelling doaj-art-3e92813741d54093a7ad7d7cda65bd232025-08-20T03:42:49ZengNature PortfolioScientific Reports2045-23222025-07-011511810.1038/s41598-025-99987-6Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apneaÖnder Otlu0Mehmet Erdem1Tuğba Raika Kıran2Feyza İnceoğlu3Ayşegül Altıntop Geçkil4Nurcan Kırıcı Berber5Erdal İn6Department of Medical Biochemistry, Faculty of Medicine, Malatya Turgut Özal UniversityDepartment of Medical Biochemistry, Faculty of Medicine, Malatya Turgut Özal UniversityDepartment of Medical Biochemistry, Faculty of Medicine, Malatya Turgut Özal UniversityDepartment of Biostatistics, Faculty of Medicine, Malatya Turgut Özal UniversityDepartment of Pulmonary Medicine, Faculty of Medicine, Malatya Turgut Özal UniversityDepartment of Pulmonary Medicine, Faculty of Medicine, Malatya Turgut Özal UniversityDepartment of Pulmonary Diseases, Faculty of Medicine, İzmir University of EconomicsAbstract Oxygen-regulated protein 150 (ORP150) is a chaperone found in the endoplasmic reticulum (ER) induced by ER stress, oxidative stress, glutamate toxicity, ischemia, and hypoxia. Increased expression of ORP150 protects the cells by stopping ER stress, maintaining calcium balance, and delaying apoptosis. In this study, we aim to investigate serum ORP150 amount in patients with different severity levels of obstructive sleep apnea (OSA). Forty-nine patients (25 of severe and 24 of mild-moderate), and 23 healthy controls were included in the study. Routine biochemical measurements and serum ORP150 measurements of all groups and sleep quality measurements of OSA groups were obtained. ELISA was used to measure ORP150 levels in serum samples. In addition, ROC analysis was performed to determine the diagnostic power of the ORP150 parameter. There are significant differences between all three groups in terms of ORP150 values (severe OSA: 8.03 ± 0.4 ng/mL; mild-moderate OSA: 5.54 ± 0.47 ng/mL; control: 4.41 ± 0.25 ng/mL, p < 0.017). The highest ORP150 level belongs to the severe OSA group and there is a direct correlation between the severity of the disease and ORP150 levels. ORP150 value is a distinguishing parameter for OSA and the cut-off value of ORP150 was observed as 7.14 ng/mL. We concluded that serum ORP150 levels can be a differential diagnostic parameter in OSA patients and that disease severity can be determined by serum ORP150 measurement.https://doi.org/10.1038/s41598-025-99987-6Apnea-hypopnea indexObstructive sleep apneaOxygen-regulated protein 150PolysomnographySleep quality
spellingShingle Önder Otlu
Mehmet Erdem
Tuğba Raika Kıran
Feyza İnceoğlu
Ayşegül Altıntop Geçkil
Nurcan Kırıcı Berber
Erdal İn
Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea
Scientific Reports
Apnea-hypopnea index
Obstructive sleep apnea
Oxygen-regulated protein 150
Polysomnography
Sleep quality
title Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea
title_full Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea
title_fullStr Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea
title_full_unstemmed Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea
title_short Oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea
title_sort oxygen regulated protein 150 can be considered as a severity indicator in obstructive sleep apnea
topic Apnea-hypopnea index
Obstructive sleep apnea
Oxygen-regulated protein 150
Polysomnography
Sleep quality
url https://doi.org/10.1038/s41598-025-99987-6
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