Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis
Objective Rheumatoid arthritis is an autoimmune disease characterised by inflammation and bone loss, leading to joint destruction and deformity. The cervus and cucumis polypeptide (CCP) injection, one of the traditional Chinese medicine injections combined extracts from deer horn and sweet melon see...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMJ Publishing Group
2019-12-01
|
| Series: | Lupus Science and Medicine |
| Online Access: | https://lupus.bmj.com/content/6/1/e000331.full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850255856166240256 |
|---|---|
| author | Ze-Min Lin Yu-Ting Liu Yan-Sheng Xu Xiao-Qian Yang Feng-Hua Zhu Wei Tang Shi-Jun He Jian-Ping Zuo |
| author_facet | Ze-Min Lin Yu-Ting Liu Yan-Sheng Xu Xiao-Qian Yang Feng-Hua Zhu Wei Tang Shi-Jun He Jian-Ping Zuo |
| author_sort | Ze-Min Lin |
| collection | DOAJ |
| description | Objective Rheumatoid arthritis is an autoimmune disease characterised by inflammation and bone loss, leading to joint destruction and deformity. The cervus and cucumis polypeptide (CCP) injection, one of the traditional Chinese medicine injections combined extracts from deer horn and sweet melon seeds, is widely used to treat arthritis and bone fracture in China. The present study investigated the therapeutic efficacy and mechanism of CCP on pathological immune cells and bone homoeostasis in rodent experimental arthritis.Methods The effects of CCP (4 mg/kg and 2 mg/kg) on clinical arthritis symptoms, bone erosion, proinflammatory cytokines and pathological immune cells induced by complete Freund’s adjuvant was evaluated in male Sprague-Dawley rats. The impacts of CCP (2 mg/kg) on joint erythema and swelling, production of pathogenic antibodies and the proportion of inflammatory cells were assessed in collagen-induced arthritis (CIA) in DBA/1J mice. Regulation of osteoclastogenesis by CCP was observed in the murine macrophage-like RAW264.7 cells treated with receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF).Results CCP administration significantly prevented disease progression in both adjuvant-induced arthritis (AIA) rats and CIA mice. The therapeutic benefits were accompanied by reduction of paw oedema, reversed bone destruction, decreased pathological changes and osteoclast numbers in joints in AIA rats, as well as attenuated clinical manifestation and autoantibodies production in CIA mice. Meanwhile, in vitro supplemented of CCP concentration dependently inhibited RANKL/M-CSF-induced osteoclast differentiation, without showing cytotoxicity in RAW264.7 cells. Further, the presence of CCP dampened the augmented downstream signalling transduction as well as activation of osteoclast-specific genes and transcription factors induced by RANKL/M-CSF in RAW264.7 cells.Conclusion Our study suggested that the therapeutic effects of CCP in experimental arthritis could be attributed to its intervention on RANKL-induced osteoclastogenesis signalling pathway in osteoclast precursor cells. |
| format | Article |
| id | doaj-art-3e8ab4fa0da540518a8ff40aa0faeaa5 |
| institution | OA Journals |
| issn | 2053-8790 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | Lupus Science and Medicine |
| spelling | doaj-art-3e8ab4fa0da540518a8ff40aa0faeaa52025-08-20T01:56:46ZengBMJ Publishing GroupLupus Science and Medicine2053-87902019-12-016110.1136/lupus-2019-000331Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesisZe-Min Lin0Yu-Ting Liu1Yan-Sheng Xu2Xiao-Qian Yang3Feng-Hua Zhu4Wei Tang5Shi-Jun He6Jian-Ping Zuo7Laboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, ChinaLaboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, ChinaLaboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaLaboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, ChinaLaboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, ChinaLaboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, ChinaLaboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, ChinaLaboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, ChinaObjective Rheumatoid arthritis is an autoimmune disease characterised by inflammation and bone loss, leading to joint destruction and deformity. The cervus and cucumis polypeptide (CCP) injection, one of the traditional Chinese medicine injections combined extracts from deer horn and sweet melon seeds, is widely used to treat arthritis and bone fracture in China. The present study investigated the therapeutic efficacy and mechanism of CCP on pathological immune cells and bone homoeostasis in rodent experimental arthritis.Methods The effects of CCP (4 mg/kg and 2 mg/kg) on clinical arthritis symptoms, bone erosion, proinflammatory cytokines and pathological immune cells induced by complete Freund’s adjuvant was evaluated in male Sprague-Dawley rats. The impacts of CCP (2 mg/kg) on joint erythema and swelling, production of pathogenic antibodies and the proportion of inflammatory cells were assessed in collagen-induced arthritis (CIA) in DBA/1J mice. Regulation of osteoclastogenesis by CCP was observed in the murine macrophage-like RAW264.7 cells treated with receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF).Results CCP administration significantly prevented disease progression in both adjuvant-induced arthritis (AIA) rats and CIA mice. The therapeutic benefits were accompanied by reduction of paw oedema, reversed bone destruction, decreased pathological changes and osteoclast numbers in joints in AIA rats, as well as attenuated clinical manifestation and autoantibodies production in CIA mice. Meanwhile, in vitro supplemented of CCP concentration dependently inhibited RANKL/M-CSF-induced osteoclast differentiation, without showing cytotoxicity in RAW264.7 cells. Further, the presence of CCP dampened the augmented downstream signalling transduction as well as activation of osteoclast-specific genes and transcription factors induced by RANKL/M-CSF in RAW264.7 cells.Conclusion Our study suggested that the therapeutic effects of CCP in experimental arthritis could be attributed to its intervention on RANKL-induced osteoclastogenesis signalling pathway in osteoclast precursor cells.https://lupus.bmj.com/content/6/1/e000331.full |
| spellingShingle | Ze-Min Lin Yu-Ting Liu Yan-Sheng Xu Xiao-Qian Yang Feng-Hua Zhu Wei Tang Shi-Jun He Jian-Ping Zuo Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis Lupus Science and Medicine |
| title | Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis |
| title_full | Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis |
| title_fullStr | Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis |
| title_full_unstemmed | Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis |
| title_short | Cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis |
| title_sort | cervus and cucumis peptides ameliorates bone erosion in experimental arthritis by inhibiting osteoclastogenesis |
| url | https://lupus.bmj.com/content/6/1/e000331.full |
| work_keys_str_mv | AT zeminlin cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis AT yutingliu cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis AT yanshengxu cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis AT xiaoqianyang cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis AT fenghuazhu cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis AT weitang cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis AT shijunhe cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis AT jianpingzuo cervusandcucumispeptidesamelioratesboneerosioninexperimentalarthritisbyinhibitingosteoclastogenesis |