DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition
Abstract Droplet interface bilayers (DIBs) offer a controlled lipid environment for studying membrane‐bound processes, with applications in artificial cells, biosensing, and biophysics. Current DIB fabrication faces challenges due to time‐consuming processes and specialized equipment, limiting scale...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-12-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400413 |
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| author | Alexander F Mason Shelley FJ Wickham Matthew AB Baker |
| author_facet | Alexander F Mason Shelley FJ Wickham Matthew AB Baker |
| author_sort | Alexander F Mason |
| collection | DOAJ |
| description | Abstract Droplet interface bilayers (DIBs) offer a controlled lipid environment for studying membrane‐bound processes, with applications in artificial cells, biosensing, and biophysics. Current DIB fabrication faces challenges due to time‐consuming processes and specialized equipment, limiting scale‐up and hindering statistical significance in single‐molecule assays. This research introduces “DIB‐BOT,” an open‐source solution combining a nanoinjector and a 3D printer. DIB‐BOT enables rapid, reproducible DIB fabrication, overcoming manual limitations. Using off‐the‐shelf components, DIB‐BOT ensures high spatial reproducibility, minimal user input, and scalable experiments. The system's utility is demonstrated through pairwise droplet assembly and a fluorescence plate‐reader assay. Compared to manual fabrication, DIB‐BOT shows a 10‐fold reduction in droplet volume error, a threefold reduction in positional error, and 100% droplet yield. This method lowers entry barriers to DIB research, expanding its applications and enhancing data quality. |
| format | Article |
| id | doaj-art-3e890341c1704ba095f731b81a95b27e |
| institution | OA Journals |
| issn | 2196-7350 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-3e890341c1704ba095f731b81a95b27e2025-08-20T02:31:36ZengWiley-VCHAdvanced Materials Interfaces2196-73502024-12-011135n/an/a10.1002/admi.202400413DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer DepositionAlexander F Mason0Shelley FJ Wickham1Matthew AB Baker2School of Biotechnology and Biomolecular Science UNSW Sydney Kensington NSW 2052 AustraliaSchool of Chemistry University of Sydney Sydney NSW 2006 AustraliaSchool of Biotechnology and Biomolecular Science UNSW Sydney Kensington NSW 2052 AustraliaAbstract Droplet interface bilayers (DIBs) offer a controlled lipid environment for studying membrane‐bound processes, with applications in artificial cells, biosensing, and biophysics. Current DIB fabrication faces challenges due to time‐consuming processes and specialized equipment, limiting scale‐up and hindering statistical significance in single‐molecule assays. This research introduces “DIB‐BOT,” an open‐source solution combining a nanoinjector and a 3D printer. DIB‐BOT enables rapid, reproducible DIB fabrication, overcoming manual limitations. Using off‐the‐shelf components, DIB‐BOT ensures high spatial reproducibility, minimal user input, and scalable experiments. The system's utility is demonstrated through pairwise droplet assembly and a fluorescence plate‐reader assay. Compared to manual fabrication, DIB‐BOT shows a 10‐fold reduction in droplet volume error, a threefold reduction in positional error, and 100% droplet yield. This method lowers entry barriers to DIB research, expanding its applications and enhancing data quality.https://doi.org/10.1002/admi.202400413artificial cellsdroplet interface bilayersmembrane biophysicsopen‐source hardwaresynthetic biology |
| spellingShingle | Alexander F Mason Shelley FJ Wickham Matthew AB Baker DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition Advanced Materials Interfaces artificial cells droplet interface bilayers membrane biophysics open‐source hardware synthetic biology |
| title | DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition |
| title_full | DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition |
| title_fullStr | DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition |
| title_full_unstemmed | DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition |
| title_short | DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition |
| title_sort | dib bot an open source hardware approach for plate integrated droplet interface bilayer deposition |
| topic | artificial cells droplet interface bilayers membrane biophysics open‐source hardware synthetic biology |
| url | https://doi.org/10.1002/admi.202400413 |
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