Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods
Four rapid, accurate and very simple derivative spectrophotometric techniques were developed for the quantitative determination of binary mixtures of estradiol (E2) and progesterone (PRG) formulated as a capsule. Method I is the first derivative zero-crossing technique, derivative amplitudes were d...
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University of Baghdad, College of Science for Women
2023-12-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7822 |
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| author | Dashty K. Ali Nabil A. Fakhre Chnar M. Rasheed |
| author_facet | Dashty K. Ali Nabil A. Fakhre Chnar M. Rasheed |
| author_sort | Dashty K. Ali |
| collection | DOAJ |
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Four rapid, accurate and very simple derivative spectrophotometric techniques were developed for the quantitative determination of binary mixtures of estradiol (E2) and progesterone (PRG) formulated as a capsule. Method I is the first derivative zero-crossing technique, derivative amplitudes were detected at the zero-crossing wavelength of 239.27 and 292.51 nm for the quantification of estradiol and 249.19 nm for Progesterone. Method II is ratio subtraction, progesterone was determined at λmax 240 nm after subtraction of interference exerted by estradiol. Method III is modified amplitude subtraction, which was established using derivative spectroscopy and mathematical manipulations. Method IIII is the absorbance ratio technique, absorbance of both medicines was measured at two wavelengths λ1= 260, -absorptive point and λ2=240max of progesterone. The Q equations were used to calculate the final concentrations. The calibration curve is linear from 5.0–140 and 2.0–32.0 µg/ml for estradiol and progesterone respectively. The proposed techniques' selectivity was tested using synthetic combinations created in the lab and assessed using the standard addition method. Using one-way ANOVA, the outputs of the proposed ways were compared, and the result showed no significant differences between the proposed techniques.
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| format | Article |
| id | doaj-art-3e88bea4d7af49a09fc31269dff0abd1 |
| institution | DOAJ |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-3e88bea4d7af49a09fc31269dff0abd12025-08-20T02:52:38ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862023-12-0120610.21123/bsj.2023.7822Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric MethodsDashty K. Ali0Nabil A. Fakhre 1Chnar M. Rasheed 2Department of Chemistry, College of Education, University of Salahaddin, Erbil, IraqDepartment of Chemistry, College of Education, University of Salahaddin, Erbil, IraqDepartment of Chemistry, College of Education, University of Salahaddin, Erbil, Iraq Four rapid, accurate and very simple derivative spectrophotometric techniques were developed for the quantitative determination of binary mixtures of estradiol (E2) and progesterone (PRG) formulated as a capsule. Method I is the first derivative zero-crossing technique, derivative amplitudes were detected at the zero-crossing wavelength of 239.27 and 292.51 nm for the quantification of estradiol and 249.19 nm for Progesterone. Method II is ratio subtraction, progesterone was determined at λmax 240 nm after subtraction of interference exerted by estradiol. Method III is modified amplitude subtraction, which was established using derivative spectroscopy and mathematical manipulations. Method IIII is the absorbance ratio technique, absorbance of both medicines was measured at two wavelengths λ1= 260, -absorptive point and λ2=240max of progesterone. The Q equations were used to calculate the final concentrations. The calibration curve is linear from 5.0–140 and 2.0–32.0 µg/ml for estradiol and progesterone respectively. The proposed techniques' selectivity was tested using synthetic combinations created in the lab and assessed using the standard addition method. Using one-way ANOVA, the outputs of the proposed ways were compared, and the result showed no significant differences between the proposed techniques. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7822Absorbance ratio method, Derivative spectrophotometry, Estradiol, Progesterone, Ratio subtraction. |
| spellingShingle | Dashty K. Ali Nabil A. Fakhre Chnar M. Rasheed Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods مجلة بغداد للعلوم Absorbance ratio method, Derivative spectrophotometry, Estradiol, Progesterone, Ratio subtraction. |
| title | Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods |
| title_full | Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods |
| title_fullStr | Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods |
| title_full_unstemmed | Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods |
| title_short | Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods |
| title_sort | simultaneous determination of binary mixture of estradiol and progesterone using different spectrophotometric methods |
| topic | Absorbance ratio method, Derivative spectrophotometry, Estradiol, Progesterone, Ratio subtraction. |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7822 |
| work_keys_str_mv | AT dashtykali simultaneousdeterminationofbinarymixtureofestradiolandprogesteroneusingdifferentspectrophotometricmethods AT nabilafakhre simultaneousdeterminationofbinarymixtureofestradiolandprogesteroneusingdifferentspectrophotometricmethods AT chnarmrasheed simultaneousdeterminationofbinarymixtureofestradiolandprogesteroneusingdifferentspectrophotometricmethods |