Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology

Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology

Summary: Inhibitory synaptic transmission mediated by the neurotransmitter γ-aminobutyric acid (GABA) is dependent on the concentration of chloride ions (Cl−) in neurons, which can be assessed by making patch-clamp recordings of the reversal potential for GABA (EGABA). Here, we present a protocol to...

Full description

Saved in:
Bibliographic Details
Main Authors: Vineeth A. Raveendran, Jessica C. Pressey, Melanie A. Woodin
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell Membrane
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000346
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items

Similar Items