Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology
Summary: Inhibitory synaptic transmission mediated by the neurotransmitter γ-aminobutyric acid (GABA) is dependent on the concentration of chloride ions (Cl−) in neurons, which can be assessed by making patch-clamp recordings of the reversal potential for GABA (EGABA). Here, we present a protocol to...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000346 |
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| author | Vineeth A. Raveendran Jessica C. Pressey Melanie A. Woodin |
| author_facet | Vineeth A. Raveendran Jessica C. Pressey Melanie A. Woodin |
| author_sort | Vineeth A. Raveendran |
| collection | DOAJ |
| description | Summary: Inhibitory synaptic transmission mediated by the neurotransmitter γ-aminobutyric acid (GABA) is dependent on the concentration of chloride ions (Cl−) in neurons, which can be assessed by making patch-clamp recordings of the reversal potential for GABA (EGABA). Here, we present a protocol to study the regulation of cation-chloride cotransporters and the strength of synaptic inhibition in cultured mouse cortical neurons using electrophysiology. We describe steps for culturing neurons isolated from postnatal pups and electrophysiological measurement of EGABA.For complete details on the use and execution of this protocol, please refer to Raveendran et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-3e856f91fe0d49cb9305c61cb5c6af55 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-3e856f91fe0d49cb9305c61cb5c6af552025-02-09T05:01:24ZengElsevierSTAR Protocols2666-16672025-03-0161103628Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiologyVineeth A. Raveendran0Jessica C. Pressey1Melanie A. Woodin2Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, CanadaDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, CanadaDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada; Corresponding authorSummary: Inhibitory synaptic transmission mediated by the neurotransmitter γ-aminobutyric acid (GABA) is dependent on the concentration of chloride ions (Cl−) in neurons, which can be assessed by making patch-clamp recordings of the reversal potential for GABA (EGABA). Here, we present a protocol to study the regulation of cation-chloride cotransporters and the strength of synaptic inhibition in cultured mouse cortical neurons using electrophysiology. We describe steps for culturing neurons isolated from postnatal pups and electrophysiological measurement of EGABA.For complete details on the use and execution of this protocol, please refer to Raveendran et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000346Cell cultureCell MembraneNeuroscience |
| spellingShingle | Vineeth A. Raveendran Jessica C. Pressey Melanie A. Woodin Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology STAR Protocols Cell culture Cell Membrane Neuroscience |
| title | Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology |
| title_full | Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology |
| title_fullStr | Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology |
| title_full_unstemmed | Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology |
| title_short | Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology |
| title_sort | protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology |
| topic | Cell culture Cell Membrane Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000346 |
| work_keys_str_mv | AT vineetharaveendran protocoltostudychlorideregulationinculturedmousecorticalneuronsusingelectrophysiology AT jessicacpressey protocoltostudychlorideregulationinculturedmousecorticalneuronsusingelectrophysiology AT melanieawoodin protocoltostudychlorideregulationinculturedmousecorticalneuronsusingelectrophysiology |