A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid Chromatography
The miniaturization of liquid chromatography (LC) columns to capillary and nanoscales allows temperature programming to be an effective alternative to solvent gradients for modulating eluotropic strength. This approach simplifies instrument design and operation, as a single pump can suffice to achie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/12/1/5 |
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| author | Lincon Coutinho Marins Alessandra Maffei Monteiro Vivane Lopes Leal Deyber Arley Vargas Medina Edwin Martin Cardenas Fernando Mauro Lanças |
| author_facet | Lincon Coutinho Marins Alessandra Maffei Monteiro Vivane Lopes Leal Deyber Arley Vargas Medina Edwin Martin Cardenas Fernando Mauro Lanças |
| author_sort | Lincon Coutinho Marins |
| collection | DOAJ |
| description | The miniaturization of liquid chromatography (LC) columns to capillary and nanoscales allows temperature programming to be an effective alternative to solvent gradients for modulating eluotropic strength. This approach simplifies instrument design and operation, as a single pump can suffice to achieve efficient separations. This study presents the development and application of a compact, lab-built high-pressure system for temperature-programmed capillary and nanoLC separations. The instrument includes a high-pressure capillary–nanoflow syringe pump, a time-based nanoliter injection system, a programmable capillary column oven for controlled temperature gradients, and a UV-Vis detection system with a custom nanoliter-scale detection cell. Each system component was designed and built in-house, with rigorous calibration to ensure accuracy and operational reliability. Experimental data confirm the system’s capability to deliver precise, reproducible temperature, and flow rates. Functionality was validated through temperature-programmed separations on packed and open tubular capillary columns. The results demonstrated that the developed instrument offers enhanced separation efficiency and reduced analysis time compared to isothermal methods, underscoring its potential for advanced applications in miniaturized liquid chromatography. |
| format | Article |
| id | doaj-art-3e8373b468344c7fa884b2ff666761a7 |
| institution | Kabale University |
| issn | 2297-8739 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj-art-3e8373b468344c7fa884b2ff666761a72025-01-24T13:49:29ZengMDPI AGSeparations2297-87392024-12-01121510.3390/separations12010005A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid ChromatographyLincon Coutinho Marins0Alessandra Maffei Monteiro1Vivane Lopes Leal2Deyber Arley Vargas Medina3Edwin Martin Cardenas4Fernando Mauro Lanças5Institute of Chemistry of São Carlos, University of São Paulo, São Carlos 05508-220, SP, BrazilInstitute of Chemistry of São Carlos, University of São Paulo, São Carlos 05508-220, SP, BrazilInstitute of Chemistry of São Carlos, University of São Paulo, São Carlos 05508-220, SP, BrazilInstitute of Chemistry of São Carlos, University of São Paulo, São Carlos 05508-220, SP, BrazilNano Separation Technologies, São Carlos 13561-140, SP, BrazilInstitute of Chemistry of São Carlos, University of São Paulo, São Carlos 05508-220, SP, BrazilThe miniaturization of liquid chromatography (LC) columns to capillary and nanoscales allows temperature programming to be an effective alternative to solvent gradients for modulating eluotropic strength. This approach simplifies instrument design and operation, as a single pump can suffice to achieve efficient separations. This study presents the development and application of a compact, lab-built high-pressure system for temperature-programmed capillary and nanoLC separations. The instrument includes a high-pressure capillary–nanoflow syringe pump, a time-based nanoliter injection system, a programmable capillary column oven for controlled temperature gradients, and a UV-Vis detection system with a custom nanoliter-scale detection cell. Each system component was designed and built in-house, with rigorous calibration to ensure accuracy and operational reliability. Experimental data confirm the system’s capability to deliver precise, reproducible temperature, and flow rates. Functionality was validated through temperature-programmed separations on packed and open tubular capillary columns. The results demonstrated that the developed instrument offers enhanced separation efficiency and reduced analysis time compared to isothermal methods, underscoring its potential for advanced applications in miniaturized liquid chromatography.https://www.mdpi.com/2297-8739/12/1/5capillary liquid chromatography (capillary LC)nanoflow liquid chromatography (nanoLC)open tubular liquid chromatography (OTLC)temperature gradient separationslaboratory-built instruments |
| spellingShingle | Lincon Coutinho Marins Alessandra Maffei Monteiro Vivane Lopes Leal Deyber Arley Vargas Medina Edwin Martin Cardenas Fernando Mauro Lanças A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid Chromatography Separations capillary liquid chromatography (capillary LC) nanoflow liquid chromatography (nanoLC) open tubular liquid chromatography (OTLC) temperature gradient separations laboratory-built instruments |
| title | A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid Chromatography |
| title_full | A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid Chromatography |
| title_fullStr | A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid Chromatography |
| title_full_unstemmed | A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid Chromatography |
| title_short | A Compact Instrument for Temperature-Programming-Assisted Capillary–Nanoliquid Chromatography |
| title_sort | compact instrument for temperature programming assisted capillary nanoliquid chromatography |
| topic | capillary liquid chromatography (capillary LC) nanoflow liquid chromatography (nanoLC) open tubular liquid chromatography (OTLC) temperature gradient separations laboratory-built instruments |
| url | https://www.mdpi.com/2297-8739/12/1/5 |
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