Comprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonica

Abstract Comprehensive expression datasets were constructed for Apis mellifera and Apis cerana japonica. Firstly, we obtained RNA-Seq data of the samples prepared: post-oviposition day 6 to day 58 samples of A. mellifera workers (larva to adult), and day 9, 10, 12, and 13 samples of A. mellifera que...

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Main Authors: Kakeru Yokoi, Masatsugu Hatakeyama, Seigo Kuwazaki, Taro Maeda, Mikio Yoshiyama, Mari Horigane-Ogihara, Shigeru Matsuyama, Akiya Jouraku, Hidemasa Bono, Kiyoshi Kimura
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05279-z
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author Kakeru Yokoi
Masatsugu Hatakeyama
Seigo Kuwazaki
Taro Maeda
Mikio Yoshiyama
Mari Horigane-Ogihara
Shigeru Matsuyama
Akiya Jouraku
Hidemasa Bono
Kiyoshi Kimura
author_facet Kakeru Yokoi
Masatsugu Hatakeyama
Seigo Kuwazaki
Taro Maeda
Mikio Yoshiyama
Mari Horigane-Ogihara
Shigeru Matsuyama
Akiya Jouraku
Hidemasa Bono
Kiyoshi Kimura
author_sort Kakeru Yokoi
collection DOAJ
description Abstract Comprehensive expression datasets were constructed for Apis mellifera and Apis cerana japonica. Firstly, we obtained RNA-Seq data of the samples prepared: post-oviposition day 6 to day 58 samples of A. mellifera workers (larva to adult), and day 9, 10, 12, and 13 samples of A. mellifera queen (larva to pupa), and day 9 to day 18 samples of A. cerana japonica workers (larva to adult). For A. cerana japonica, reference transcript sequence, predicted amino acid sequence, and functional annotation data were generated based on the genome sequence and RNA-Seq data. Using the transcript sequence and RNA-Seq data, comprehensive expression data for all transcripts of A. mellifera and A. cerana japonica were prepared. Hierarchical clustering analyses, the used sample preparation method, and other evaluation results ensured that both sets of expression data were reliable for use as comprehensive reference expression datasets. Therefore, these data can be used for honey bee research or comparative or evolutionary studies on insect species or social insect species at the genetic and molecular levels.
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spelling doaj-art-3e7bcdbff2f7465b9b6d49457441841d2025-08-20T02:30:43ZengNature PortfolioScientific Data2052-44632025-06-011211810.1038/s41597-025-05279-zComprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonicaKakeru Yokoi0Masatsugu Hatakeyama1Seigo Kuwazaki2Taro Maeda3Mikio Yoshiyama4Mari Horigane-Ogihara5Shigeru Matsuyama6Akiya Jouraku7Hidemasa Bono8Kiyoshi Kimura9Insect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO)Insect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO)Insect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO)Biodiversity Conservation and Utilization Group, Division of Agroecosystem Management Research, Institute of Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO)Smart Livestock Facilities Group, Division of Advanced Feeding Technology Research, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)Smart Livestock Facilities Group, Division of Advanced Feeding Technology Research, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)Graduate School of Life and Environmental Sciences, University of TsukubaInsect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO)Laboratory of BioDX, PtBio Co-Creation Research Center, Genome Editing Innovation Center, Hiroshima UniversityInsect Design Technology Group, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO)Abstract Comprehensive expression datasets were constructed for Apis mellifera and Apis cerana japonica. Firstly, we obtained RNA-Seq data of the samples prepared: post-oviposition day 6 to day 58 samples of A. mellifera workers (larva to adult), and day 9, 10, 12, and 13 samples of A. mellifera queen (larva to pupa), and day 9 to day 18 samples of A. cerana japonica workers (larva to adult). For A. cerana japonica, reference transcript sequence, predicted amino acid sequence, and functional annotation data were generated based on the genome sequence and RNA-Seq data. Using the transcript sequence and RNA-Seq data, comprehensive expression data for all transcripts of A. mellifera and A. cerana japonica were prepared. Hierarchical clustering analyses, the used sample preparation method, and other evaluation results ensured that both sets of expression data were reliable for use as comprehensive reference expression datasets. Therefore, these data can be used for honey bee research or comparative or evolutionary studies on insect species or social insect species at the genetic and molecular levels.https://doi.org/10.1038/s41597-025-05279-z
spellingShingle Kakeru Yokoi
Masatsugu Hatakeyama
Seigo Kuwazaki
Taro Maeda
Mikio Yoshiyama
Mari Horigane-Ogihara
Shigeru Matsuyama
Akiya Jouraku
Hidemasa Bono
Kiyoshi Kimura
Comprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonica
Scientific Data
title Comprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonica
title_full Comprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonica
title_fullStr Comprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonica
title_full_unstemmed Comprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonica
title_short Comprehensive expression data for two honey bee species, Apis mellifera and Apis cerana japonica
title_sort comprehensive expression data for two honey bee species apis mellifera and apis cerana japonica
url https://doi.org/10.1038/s41597-025-05279-z
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