Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer
Background: Bone metastasis remains one of the biggest challenges in the treatment of prostate cancer, and other solid tumors such as breast, lung, and colon. Modeling a complex microenvironment in-vitro such as the bone niche, requires interrogation of cell-cell interactions, specific extracellular...
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025003093 |
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| author | Rebeca San Martin Prijoyit Das Tianchun Xue Morgan Rose Brown Renata Dos Reis Marques Michael Essington Adrian Gonzalez Rachel Patton McCord |
| author_facet | Rebeca San Martin Prijoyit Das Tianchun Xue Morgan Rose Brown Renata Dos Reis Marques Michael Essington Adrian Gonzalez Rachel Patton McCord |
| author_sort | Rebeca San Martin |
| collection | DOAJ |
| description | Background: Bone metastasis remains one of the biggest challenges in the treatment of prostate cancer, and other solid tumors such as breast, lung, and colon. Modeling a complex microenvironment in-vitro such as the bone niche, requires interrogation of cell-cell interactions, specific extracellular matrix proteins, and a high calcium environment. Methods: Here, we present a fast and cost-effective system in which commercially available, non-adhesive cell culture vessels are coated with amorphous calcium phosphate (ACP) as a surrogate for bone matrix. We also present modified protocols for subculturing cells and collecting nucleic acids and protein in high-calcium samples. Results: We find that prostate epithelial cell lines show increased adhesion and proliferation when cultured in these amorphous calcium surfaces, accompanied by independence from androgen starvation. We observe gene expression changes on ACP surfaces in early adenocarcinoma cell lines which match alterations relevant to prostate cancer progression. Conclusions: Incorporating biologically relevant in-vitro systems that address the microenvironment milieu of the metastatic site is essential for accurately modeling cancer progression. In the case of bone metastasis, calcium availability, uptake, and downstream signaling are of paramount importance for the survival of the cancer cell and should be considered in the development of pre-clinical models. |
| format | Article |
| id | doaj-art-3e71474fa863465a96d5d8610f75aa7a |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-3e71474fa863465a96d5d8610f75aa7a2025-01-28T04:14:45ZengElsevierHeliyon2405-84402025-02-01113e41929Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancerRebeca San Martin0Prijoyit Das1Tianchun Xue2Morgan Rose Brown3Renata Dos Reis Marques4Michael Essington5Adrian Gonzalez6Rachel Patton McCord7Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, 309 Ken and Blaire Mossman Bldg. 1311 Cumberland Ave, Knoxville, TN, 37996, USAUT-ORNL Graduate School of Genome Science and Technology, University of Tennessee, 309 Ken and Blaire Mossman Bldg. 1311 Cumberland Ave, Knoxville, TN, 37996, USAUT-ORNL Graduate School of Genome Science and Technology, University of Tennessee, 309 Ken and Blaire Mossman Bldg. 1311 Cumberland Ave, Knoxville, TN, 37996, USADepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee, 309 Ken and Blaire Mossman Bldg. 1311 Cumberland Ave, Knoxville, TN, 37996, USADepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee, 309 Ken and Blaire Mossman Bldg. 1311 Cumberland Ave, Knoxville, TN, 37996, USADepartment of Biosystems Engineering and Soil Science. University of Tennessee, Institute of Agriculture, 2621 Morgan Circle, Knoxville, TN, 37996, USAWater Quality Core Facility. Department of Civil and Environmental Engineering, Tickle College of Engineering. University of Tennessee, 325 John D. Tickle Engineering Building 851 Neyland Drive, Knoxville, TN, 37996, USADepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee, 309 Ken and Blaire Mossman Bldg. 1311 Cumberland Ave, Knoxville, TN, 37996, USA; Corresponding author.Background: Bone metastasis remains one of the biggest challenges in the treatment of prostate cancer, and other solid tumors such as breast, lung, and colon. Modeling a complex microenvironment in-vitro such as the bone niche, requires interrogation of cell-cell interactions, specific extracellular matrix proteins, and a high calcium environment. Methods: Here, we present a fast and cost-effective system in which commercially available, non-adhesive cell culture vessels are coated with amorphous calcium phosphate (ACP) as a surrogate for bone matrix. We also present modified protocols for subculturing cells and collecting nucleic acids and protein in high-calcium samples. Results: We find that prostate epithelial cell lines show increased adhesion and proliferation when cultured in these amorphous calcium surfaces, accompanied by independence from androgen starvation. We observe gene expression changes on ACP surfaces in early adenocarcinoma cell lines which match alterations relevant to prostate cancer progression. Conclusions: Incorporating biologically relevant in-vitro systems that address the microenvironment milieu of the metastatic site is essential for accurately modeling cancer progression. In the case of bone metastasis, calcium availability, uptake, and downstream signaling are of paramount importance for the survival of the cancer cell and should be considered in the development of pre-clinical models.http://www.sciencedirect.com/science/article/pii/S2405844025003093Prostate cancerBone metastasisAmorphous calcium phosphateCell culture modelingAndrogen resistanceGene expression |
| spellingShingle | Rebeca San Martin Prijoyit Das Tianchun Xue Morgan Rose Brown Renata Dos Reis Marques Michael Essington Adrian Gonzalez Rachel Patton McCord Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer Heliyon Prostate cancer Bone metastasis Amorphous calcium phosphate Cell culture modeling Androgen resistance Gene expression |
| title | Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer |
| title_full | Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer |
| title_fullStr | Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer |
| title_full_unstemmed | Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer |
| title_short | Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer |
| title_sort | amorphous calcium phosphate coated surfaces as a model for bone microenvironment in prostate cancer |
| topic | Prostate cancer Bone metastasis Amorphous calcium phosphate Cell culture modeling Androgen resistance Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025003093 |
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