Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in Mice

In this study, the traditional cookie recipe was optimized to develop healthier, defatted soybean flour cookies. Low-gluten flour was used as the primary ingredient, with defatted soybean flour as a supplement. Butter and sucrose were partially replaced with grape seed oil and fructooligosaccharides...

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Main Authors: Liangliang DUAN, Siqing ZHENG, Boya ZHAO, Yuqing WANG, Hongze JIA, Jin SHANG, Shouzhu XU, Dongqing YE, Changbao LI, Chuanyan RAO
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-04-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050158
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author Liangliang DUAN
Siqing ZHENG
Boya ZHAO
Yuqing WANG
Hongze JIA
Jin SHANG
Shouzhu XU
Dongqing YE
Changbao LI
Chuanyan RAO
author_facet Liangliang DUAN
Siqing ZHENG
Boya ZHAO
Yuqing WANG
Hongze JIA
Jin SHANG
Shouzhu XU
Dongqing YE
Changbao LI
Chuanyan RAO
author_sort Liangliang DUAN
collection DOAJ
description In this study, the traditional cookie recipe was optimized to develop healthier, defatted soybean flour cookies. Low-gluten flour was used as the primary ingredient, with defatted soybean flour as a supplement. Butter and sucrose were partially replaced with grape seed oil and fructooligosaccharides, respectively. Single-factor and response surface methodology experiments were conducted. The final formulation was determined based on sensory evaluation, texture analysis, and impact on lipid metabolism in mice. The results indicated that the optimized formulation included 62.24 g low-gluten flour, 37.76 g defatted soybean flour, 42.66 g butter, 12.34 g grape seed oil, 19.61 g sucrose, 5.39 g fructooligosaccharides, and 35.00 g egg. The sensory score of the product was 90.40±4.69 higher than the traditional cookie's score of 83.08±4.85 (P<0.05). Although the cost of the optimized formulation was 1.40~1.50 times higher than that of the traditional formulation, it did not disrupt lipid metabolism in BALB/c mice. After 12 weeks of feeding, the defatted soybean flour cookie group mice exhibited significantly lower body weight, liver index, fat coefficient and serum liver damage indicators than those in the traditional cookie group (P<0.05). Total triglycerides (TG) and low density lipoprotein cholesterol (LDL-C) of defatted soybean flour cookie group mice were significantly lower than the traditional cookie group while high density lipoprotein cholesterol (HDL-C) was significantly higher than the traditional cookie group (P<0.05). No significant lipid accumulation was observed in the liver tissues, similar to the observation in the maintenance diet group. These findings suggest that defatted soybean flour cookies are healthier and have promising market potential.
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institution Kabale University
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publishDate 2025-04-01
publisher The editorial department of Science and Technology of Food Industry
record_format Article
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spelling doaj-art-3e6f22383bda4e1498d75f9b7b98729e2025-08-20T03:40:00ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-04-0146719820610.13386/j.issn1002-0306.20240501582024050158-7Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in MiceLiangliang DUAN0Siqing ZHENG1Boya ZHAO2Yuqing WANG3Hongze JIA4Jin SHANG5Shouzhu XU6Dongqing YE7Changbao LI8Chuanyan RAO9Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaShaanxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaShaanxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaShaanxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaShaanxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaShaanxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaShaanxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaGuangxi Key Laboratory of New Technologies for Fruit and Vegetable Storage and Processing, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of New Technologies for Fruit and Vegetable Storage and Processing, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of New Technologies for Fruit and Vegetable Storage and Processing, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaIn this study, the traditional cookie recipe was optimized to develop healthier, defatted soybean flour cookies. Low-gluten flour was used as the primary ingredient, with defatted soybean flour as a supplement. Butter and sucrose were partially replaced with grape seed oil and fructooligosaccharides, respectively. Single-factor and response surface methodology experiments were conducted. The final formulation was determined based on sensory evaluation, texture analysis, and impact on lipid metabolism in mice. The results indicated that the optimized formulation included 62.24 g low-gluten flour, 37.76 g defatted soybean flour, 42.66 g butter, 12.34 g grape seed oil, 19.61 g sucrose, 5.39 g fructooligosaccharides, and 35.00 g egg. The sensory score of the product was 90.40±4.69 higher than the traditional cookie's score of 83.08±4.85 (P<0.05). Although the cost of the optimized formulation was 1.40~1.50 times higher than that of the traditional formulation, it did not disrupt lipid metabolism in BALB/c mice. After 12 weeks of feeding, the defatted soybean flour cookie group mice exhibited significantly lower body weight, liver index, fat coefficient and serum liver damage indicators than those in the traditional cookie group (P<0.05). Total triglycerides (TG) and low density lipoprotein cholesterol (LDL-C) of defatted soybean flour cookie group mice were significantly lower than the traditional cookie group while high density lipoprotein cholesterol (HDL-C) was significantly higher than the traditional cookie group (P<0.05). No significant lipid accumulation was observed in the liver tissues, similar to the observation in the maintenance diet group. These findings suggest that defatted soybean flour cookies are healthier and have promising market potential.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050158defatted soybean flourcookieoptimization of recipemicelipid metabolism
spellingShingle Liangliang DUAN
Siqing ZHENG
Boya ZHAO
Yuqing WANG
Hongze JIA
Jin SHANG
Shouzhu XU
Dongqing YE
Changbao LI
Chuanyan RAO
Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in Mice
Shipin gongye ke-ji
defatted soybean flour
cookie
optimization of recipe
mice
lipid metabolism
title Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in Mice
title_full Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in Mice
title_fullStr Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in Mice
title_full_unstemmed Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in Mice
title_short Optimization of the Recipe of Defatted Soybean Flour Cookies and Its Effect on Lipid Metabolism in Mice
title_sort optimization of the recipe of defatted soybean flour cookies and its effect on lipid metabolism in mice
topic defatted soybean flour
cookie
optimization of recipe
mice
lipid metabolism
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050158
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