Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit
Guava crystal is a fruit that has a high selling value. One of the obstacles to marketing crystal guava fruit is the fast decay process. Many factors cause fruit to rot quickly, including microbial contamination post-harvesting. This study evaluates the effectiveness of adding lemongrass oil in chi...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2024-11-01
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| Series: | Jurnal Ilmu Pertanian Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/JIPI/article/view/44528 |
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| author | Syarifah Meutiah Eka Sari Irma Suparto Wulan Tri Wahyuni |
| author_facet | Syarifah Meutiah Eka Sari Irma Suparto Wulan Tri Wahyuni |
| author_sort | Syarifah Meutiah Eka Sari |
| collection | DOAJ |
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Guava crystal is a fruit that has a high selling value. One of the obstacles to marketing crystal guava fruit is the fast decay process. Many factors cause fruit to rot quickly, including microbial contamination post-harvesting. This study evaluates the effectiveness of adding lemongrass oil in chitosan coatings to protect crystal guava from microbiological damage during storage. The fruit is coated with 1% chitosan and added to lemongrass oil according to the treatment. The study used a completely randomized design with three replications. The treatment tested included 0.0, 0.1, 0.3, and 0.5% lemongrass oil. The content of lemongrass oil compounds was analyzed using gas chromatography-mass spectroscopy (GC-MS). The antibacterial test was carried out using the disc method. The parameters observed were the total plate count and total fungal count of crystal guava fruit on days 0, 3, 6, 9, 12, 15, and 18 after storage. GC-MS analysis showed that the main content of lemongrass oil is two isomers of citral, i.e., neral (38.54%) and geranial (39.26%). The results showed that the total fungal and plate count increased during storage. Chitosan coating with lemongrass oil more effectively inhibits Staphylococcus aureus than Escherichia coli. Adding 0.3% and 0.5% lemongrass oil in chitosan could restrain the rate of microbes in crystal guava until the 18th day after storage. Chitosan treatment with 0,3% lemongrass oil is recommended to be the best treatment for applying on crystal guava.
Keywords: citral, GC-MS, Psidium guajava L., total plate count, total fungal count
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| format | Article |
| id | doaj-art-3e6ca88b68114bb8bc7673cc625be2aa |
| institution | DOAJ |
| issn | 0853-4217 2443-3462 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Jurnal Ilmu Pertanian Indonesia |
| spelling | doaj-art-3e6ca88b68114bb8bc7673cc625be2aa2025-08-20T02:39:50ZengBogor Agricultural UniversityJurnal Ilmu Pertanian Indonesia0853-42172443-34622024-11-0130110.18343/jipi.30.1.131Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava FruitSyarifah Meutiah Eka Sari0Irma Suparto1Wulan Tri Wahyuni21 Department of Chemistry Education, Faculty of Teacher Training and Education, Lambung Mangkurat University, Banjarmasin 70123 IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IPB Campus Darmaga, Bogor 16680 IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IPB Campus Darmaga, Bogor 16680 Indonesia Guava crystal is a fruit that has a high selling value. One of the obstacles to marketing crystal guava fruit is the fast decay process. Many factors cause fruit to rot quickly, including microbial contamination post-harvesting. This study evaluates the effectiveness of adding lemongrass oil in chitosan coatings to protect crystal guava from microbiological damage during storage. The fruit is coated with 1% chitosan and added to lemongrass oil according to the treatment. The study used a completely randomized design with three replications. The treatment tested included 0.0, 0.1, 0.3, and 0.5% lemongrass oil. The content of lemongrass oil compounds was analyzed using gas chromatography-mass spectroscopy (GC-MS). The antibacterial test was carried out using the disc method. The parameters observed were the total plate count and total fungal count of crystal guava fruit on days 0, 3, 6, 9, 12, 15, and 18 after storage. GC-MS analysis showed that the main content of lemongrass oil is two isomers of citral, i.e., neral (38.54%) and geranial (39.26%). The results showed that the total fungal and plate count increased during storage. Chitosan coating with lemongrass oil more effectively inhibits Staphylococcus aureus than Escherichia coli. Adding 0.3% and 0.5% lemongrass oil in chitosan could restrain the rate of microbes in crystal guava until the 18th day after storage. Chitosan treatment with 0,3% lemongrass oil is recommended to be the best treatment for applying on crystal guava. Keywords: citral, GC-MS, Psidium guajava L., total plate count, total fungal count https://journal.ipb.ac.id/index.php/JIPI/article/view/44528 |
| spellingShingle | Syarifah Meutiah Eka Sari Irma Suparto Wulan Tri Wahyuni Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit Jurnal Ilmu Pertanian Indonesia |
| title | Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit |
| title_full | Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit |
| title_fullStr | Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit |
| title_full_unstemmed | Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit |
| title_short | Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit |
| title_sort | application of lemongrass oil in chitosan as antimicrobial during storage of crystal guava fruit |
| url | https://journal.ipb.ac.id/index.php/JIPI/article/view/44528 |
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