<i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed Tomography

Anatomy is critical for understanding the physiological and biological adaptations of living creatures. In the case of <i>Pseudopus apodus</i>, an anguimorph lizard belonging to the Order Squamata, it is particularly important considering the scarce previous works on the morphology of it...

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Main Authors: María Isabel García-Real, Encarnación Fernández-Valle, Sara Jiménez, María José Ruiz-Fernández, David Castejón-Ferrer, Andrés Montesinos-Barceló, María Ardiaca-García, Nerea Moreno, Juncal González-Soriano
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Language:English
Published: MDPI AG 2025-02-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/5/615
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author María Isabel García-Real
Encarnación Fernández-Valle
Sara Jiménez
María José Ruiz-Fernández
David Castejón-Ferrer
Andrés Montesinos-Barceló
María Ardiaca-García
Nerea Moreno
Juncal González-Soriano
author_facet María Isabel García-Real
Encarnación Fernández-Valle
Sara Jiménez
María José Ruiz-Fernández
David Castejón-Ferrer
Andrés Montesinos-Barceló
María Ardiaca-García
Nerea Moreno
Juncal González-Soriano
author_sort María Isabel García-Real
collection DOAJ
description Anatomy is critical for understanding the physiological and biological adaptations of living creatures. In the case of <i>Pseudopus apodus</i>, an anguimorph lizard belonging to the Order Squamata, it is particularly important considering the scarce previous works on the morphology of its coelomic cavity. It is interesting to consider that, over the years, using non-invasive approaches in reptiles, such as diagnostic imaging methods, is becoming popular for both scientific and clinical purposes. For the present work, we used a total of five <i>Pseudopus apodus</i> individuals (two males and three females); one male and one female were whole-body examined by multi-detector computed tomography (CT) and then all were dissected following a conventional anatomical protocol. The novelty and the main contribution of our multi-detector CT study is to identify structures that had never been identified before using this technique, such as the opening of the vomeronasal organ and the choanae, the tongue, the glottis, the hyoid bone, the esophagus, the stomach, the small and large intestines, the cloaca, the liver, the gallbladder, the kidneys, the ovarian follicles, the trachea, the bronchial bifurcation, the lungs, the heart, the aortic arches, the aorta, the sinus venosus, and the cranial cava veins. On the contrary, other organs like the thyroid, the pancreas, the spleen, the ureters, the urinary bladder, the oviducts, the testes, the hemipenes, the pulmonary trunk, and the pulmonary arteries were only identified in the anatomical dissection. Thus, our results demonstrate that multi-detector CT scanning is a useful tool to identify a significant number of anatomical structures in <i>Pseudopus apodus</i>, which is important for clinical veterinary practice related to this species or different conservation programs, among other applications.
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spelling doaj-art-3e6c00ae42de4cf1bf2c48eb8e9e63ae2025-08-20T02:05:21ZengMDPI AGAnimals2076-26152025-02-0115561510.3390/ani15050615<i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed TomographyMaría Isabel García-Real0Encarnación Fernández-Valle1Sara Jiménez2María José Ruiz-Fernández3David Castejón-Ferrer4Andrés Montesinos-Barceló5María Ardiaca-García6Nerea Moreno7Juncal González-Soriano8Department of Animal Medicine and Surgery, Faculty of Veterinary, Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, SpainICTS Bioimagen Complutense, Complutense University, Paseo de Juan XXIII 1, 28040 Madrid, SpainAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), 48940 Leioa, SpainDepartment of Animal Medicine and Surgery, Faculty of Veterinary, Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, SpainICTS Bioimagen Complutense, Complutense University, Paseo de Juan XXIII 1, 28040 Madrid, SpainDepartment of Animal Medicine and Surgery, Faculty of Veterinary, Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, SpainMedivet 24 Horas Los Sauces, Chamberi, Calle de Sta Engracia, 63, Chamberí, 28010 Madrid, SpainDepartment of Cell Biology, Faculty of Biological Sciences, Complutense University, Avenida José Antonio Nováis 12, 28040 Madrid, SpainDepartment Section of Anatomy and Embriology, Faculty of Veterinary, Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, SpainAnatomy is critical for understanding the physiological and biological adaptations of living creatures. In the case of <i>Pseudopus apodus</i>, an anguimorph lizard belonging to the Order Squamata, it is particularly important considering the scarce previous works on the morphology of its coelomic cavity. It is interesting to consider that, over the years, using non-invasive approaches in reptiles, such as diagnostic imaging methods, is becoming popular for both scientific and clinical purposes. For the present work, we used a total of five <i>Pseudopus apodus</i> individuals (two males and three females); one male and one female were whole-body examined by multi-detector computed tomography (CT) and then all were dissected following a conventional anatomical protocol. The novelty and the main contribution of our multi-detector CT study is to identify structures that had never been identified before using this technique, such as the opening of the vomeronasal organ and the choanae, the tongue, the glottis, the hyoid bone, the esophagus, the stomach, the small and large intestines, the cloaca, the liver, the gallbladder, the kidneys, the ovarian follicles, the trachea, the bronchial bifurcation, the lungs, the heart, the aortic arches, the aorta, the sinus venosus, and the cranial cava veins. On the contrary, other organs like the thyroid, the pancreas, the spleen, the ureters, the urinary bladder, the oviducts, the testes, the hemipenes, the pulmonary trunk, and the pulmonary arteries were only identified in the anatomical dissection. Thus, our results demonstrate that multi-detector CT scanning is a useful tool to identify a significant number of anatomical structures in <i>Pseudopus apodus</i>, which is important for clinical veterinary practice related to this species or different conservation programs, among other applications.https://www.mdpi.com/2076-2615/15/5/615reptiles<i>Pseudopus apodus</i>anatomycomputed tomography
spellingShingle María Isabel García-Real
Encarnación Fernández-Valle
Sara Jiménez
María José Ruiz-Fernández
David Castejón-Ferrer
Andrés Montesinos-Barceló
María Ardiaca-García
Nerea Moreno
Juncal González-Soriano
<i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed Tomography
Animals
reptiles
<i>Pseudopus apodus</i>
anatomy
computed tomography
title <i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed Tomography
title_full <i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed Tomography
title_fullStr <i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed Tomography
title_full_unstemmed <i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed Tomography
title_short <i>Pseudopus apodus</i> Soft Tissue Anatomy Based on Comparison of Classical Dissection and Multi-Detector Computed Tomography
title_sort i pseudopus apodus i soft tissue anatomy based on comparison of classical dissection and multi detector computed tomography
topic reptiles
<i>Pseudopus apodus</i>
anatomy
computed tomography
url https://www.mdpi.com/2076-2615/15/5/615
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