Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from Yunnan
To evaluate the chemical constituents and the antioxidant and anti-inflammatory properties of Chaenomeles speciosa (Sweet) Nakai from Yunnan, extracts were prepared via heated reflux extraction, followed by physicochemical characterization using a physicochemical analysis, including LC-MS and HPLC t...
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The editorial department of Science and Technology of Food Industry
2025-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070200 |
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| author | Bo PENG Shishuai YU Liping QU Yining HAO |
| author_facet | Bo PENG Shishuai YU Liping QU Yining HAO |
| author_sort | Bo PENG |
| collection | DOAJ |
| description | To evaluate the chemical constituents and the antioxidant and anti-inflammatory properties of Chaenomeles speciosa (Sweet) Nakai from Yunnan, extracts were prepared via heated reflux extraction, followed by physicochemical characterization using a physicochemical analysis, including LC-MS and HPLC to identify primary active constituents. The antioxidant effectiveness of CE was assessed through various methods: Scavenging DPPH·, ABTS+·, and ·OH radicals, reducing iron ions, and mitigating UVA-induced ROS in HaCaT cells. The anti-inflammatory efficacy of CE was tested using an LPS-induced inflammation model in RAW264.7 cells. Results showed that CE contained 122.30 mg/g of polyphenols and 131.46 mg/g of flavonoids, with chlorogenic acid being a significant component. CE demonstrated significant efficacy in scavenging DPPH·, ABTS+·, and ·OH radicals, with IC50 values recorded at 79.58, 482.96, and 804.99 μg/mL, respectively. And it exhibited notable iron ion-reducing capacity, as evidenced by a FRAP value of 1.23 mmol Fe2+/g. Additionally, CE extremely significantly inhibited ROS production (P<0.001) in HaCaT cells in a dose-dependent manner and reduced IL-6 expression (P<0.001) and NO secretion (P<0.001) in RAW264.7 cells, indicating its potent antioxidant and anti-inflammatory effects. Overall, CE showed promising antioxidant and anti-inflammatory properties, supporting its potential therapeutic applications and providing a scientific basis for further development of Chaenomeles speciosa (Sweet) Nakai. |
| format | Article |
| id | doaj-art-3e67d129ed8b4ba5b4d3377ecb30780a |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-3e67d129ed8b4ba5b4d3377ecb30780a2025-08-20T03:46:53ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-01461230431210.13386/j.issn1002-0306.20240702002024070200-12Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from YunnanBo PENG0Shishuai YU1Liping QU2Yining HAO3Yunnan Botanee Bio-technology Group Co., Ltd., Kunming 650106, ChinaYunnan Botanee Bio-technology Group Co., Ltd., Kunming 650106, ChinaYunnan Botanee Bio-technology Group Co., Ltd., Kunming 650106, ChinaYunnan Botanee Bio-technology Group Co., Ltd., Kunming 650106, ChinaTo evaluate the chemical constituents and the antioxidant and anti-inflammatory properties of Chaenomeles speciosa (Sweet) Nakai from Yunnan, extracts were prepared via heated reflux extraction, followed by physicochemical characterization using a physicochemical analysis, including LC-MS and HPLC to identify primary active constituents. The antioxidant effectiveness of CE was assessed through various methods: Scavenging DPPH·, ABTS+·, and ·OH radicals, reducing iron ions, and mitigating UVA-induced ROS in HaCaT cells. The anti-inflammatory efficacy of CE was tested using an LPS-induced inflammation model in RAW264.7 cells. Results showed that CE contained 122.30 mg/g of polyphenols and 131.46 mg/g of flavonoids, with chlorogenic acid being a significant component. CE demonstrated significant efficacy in scavenging DPPH·, ABTS+·, and ·OH radicals, with IC50 values recorded at 79.58, 482.96, and 804.99 μg/mL, respectively. And it exhibited notable iron ion-reducing capacity, as evidenced by a FRAP value of 1.23 mmol Fe2+/g. Additionally, CE extremely significantly inhibited ROS production (P<0.001) in HaCaT cells in a dose-dependent manner and reduced IL-6 expression (P<0.001) and NO secretion (P<0.001) in RAW264.7 cells, indicating its potent antioxidant and anti-inflammatory effects. Overall, CE showed promising antioxidant and anti-inflammatory properties, supporting its potential therapeutic applications and providing a scientific basis for further development of Chaenomeles speciosa (Sweet) Nakai.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070200chenomeles speciosa (sweet) nakai from yunnanchlorogenic acidantioxidantanti-inflammatory |
| spellingShingle | Bo PENG Shishuai YU Liping QU Yining HAO Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from Yunnan Shipin gongye ke-ji chenomeles speciosa (sweet) nakai from yunnan chlorogenic acid antioxidant anti-inflammatory |
| title | Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from Yunnan |
| title_full | Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from Yunnan |
| title_fullStr | Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from Yunnan |
| title_full_unstemmed | Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from Yunnan |
| title_short | Chemical Constituents, Antioxidant, and Anti-inflammatory Activities of Chaenomeles speciosa (Sweet) Nakai Extract from Yunnan |
| title_sort | chemical constituents antioxidant and anti inflammatory activities of chaenomeles speciosa sweet nakai extract from yunnan |
| topic | chenomeles speciosa (sweet) nakai from yunnan chlorogenic acid antioxidant anti-inflammatory |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070200 |
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