405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma
Abstract Added safety measures coupled with the development and use of pathogen reduction technologies (PRT) significantly reduces the risk of transfusion-transmitted infections (TTIs) from blood products. Current approved PRTs utilize chemical and/or UV-light based inactivation methods. While the e...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83171-3 |
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| author | Joseph W. Jackson Pravin R. Kaldhone Caitlin Stewart John Anderson Scott MacGregor Michelle Maclean Marian Major Chintamani D. Atreya |
| author_facet | Joseph W. Jackson Pravin R. Kaldhone Caitlin Stewart John Anderson Scott MacGregor Michelle Maclean Marian Major Chintamani D. Atreya |
| author_sort | Joseph W. Jackson |
| collection | DOAJ |
| description | Abstract Added safety measures coupled with the development and use of pathogen reduction technologies (PRT) significantly reduces the risk of transfusion-transmitted infections (TTIs) from blood products. Current approved PRTs utilize chemical and/or UV-light based inactivation methods. While the effectiveness of these PRTs in reducing pathogens are well documented, these can cause tolerable yet unintended consequences on the quality and efficacy of the transfusion products. As an alternative to UV-based approaches, we have previously demonstrated that 405 nm violet-blue light exposure successfully inactivates a variety of pathogens, including bacteria, parasites, and viruses, in both platelet concentrates (PCs) and plasma. Herein, we show that 405 nm light treatment effectively inactivates hepatitis C cell culture virus (HCVcc) by up to ~ 3.8 log10 in small volumes of a variety of matrices, such as cell culture media, PBS, plasma, and PCs with 27 J/cm2 of light exposure, and total inactivation of HCVcc after 162 J/cm2 light exposure. Furthermore, we demonstrate that carry-over of media supplemented with fetal bovine serum enhances the production of reactive oxygen species (ROS), providing mechanistic insights to 405 nm light-mediated viral inactivation. Overall, 405 nm light successfully inactivates HCVcc, further strengthening this method as a novel PRT for platelets and plasma. |
| format | Article |
| id | doaj-art-3e5fd73d714f41f089f53cf3612ce950 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-3e5fd73d714f41f089f53cf3612ce9502025-08-20T02:43:33ZengNature PortfolioScientific Reports2045-23222024-12-011411810.1038/s41598-024-83171-3405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasmaJoseph W. Jackson0Pravin R. Kaldhone1Caitlin Stewart2John Anderson3Scott MacGregor4Michelle Maclean5Marian Major6Chintamani D. Atreya7Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDADivision of Blood Components and Devices, Center for Biologics Evaluation and Research, FDADepartment of Electronic and Electrical Engineering, The Robertson Trust Laboratory for Electronic Sterilization Technologies (ROLEST), University of StrathclydeDepartment of Electronic and Electrical Engineering, The Robertson Trust Laboratory for Electronic Sterilization Technologies (ROLEST), University of StrathclydeDepartment of Electronic and Electrical Engineering, The Robertson Trust Laboratory for Electronic Sterilization Technologies (ROLEST), University of StrathclydeDepartment of Electronic and Electrical Engineering, The Robertson Trust Laboratory for Electronic Sterilization Technologies (ROLEST), University of StrathclydeDivision of Viral Products, Center for Biologics Evaluation and Research, FDADivision of Blood Components and Devices, Center for Biologics Evaluation and Research, FDAAbstract Added safety measures coupled with the development and use of pathogen reduction technologies (PRT) significantly reduces the risk of transfusion-transmitted infections (TTIs) from blood products. Current approved PRTs utilize chemical and/or UV-light based inactivation methods. While the effectiveness of these PRTs in reducing pathogens are well documented, these can cause tolerable yet unintended consequences on the quality and efficacy of the transfusion products. As an alternative to UV-based approaches, we have previously demonstrated that 405 nm violet-blue light exposure successfully inactivates a variety of pathogens, including bacteria, parasites, and viruses, in both platelet concentrates (PCs) and plasma. Herein, we show that 405 nm light treatment effectively inactivates hepatitis C cell culture virus (HCVcc) by up to ~ 3.8 log10 in small volumes of a variety of matrices, such as cell culture media, PBS, plasma, and PCs with 27 J/cm2 of light exposure, and total inactivation of HCVcc after 162 J/cm2 light exposure. Furthermore, we demonstrate that carry-over of media supplemented with fetal bovine serum enhances the production of reactive oxygen species (ROS), providing mechanistic insights to 405 nm light-mediated viral inactivation. Overall, 405 nm light successfully inactivates HCVcc, further strengthening this method as a novel PRT for platelets and plasma.https://doi.org/10.1038/s41598-024-83171-3Pathogen inactivationHCVPlateletsPlasmablue light405 nm light |
| spellingShingle | Joseph W. Jackson Pravin R. Kaldhone Caitlin Stewart John Anderson Scott MacGregor Michelle Maclean Marian Major Chintamani D. Atreya 405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma Scientific Reports Pathogen inactivation HCV Platelets Plasma blue light 405 nm light |
| title | 405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma |
| title_full | 405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma |
| title_fullStr | 405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma |
| title_full_unstemmed | 405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma |
| title_short | 405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma |
| title_sort | 405 nm violet blue light inactivates hepatitis c cell culture virus hcvcc in ex vivo human platelet concentrates and plasma |
| topic | Pathogen inactivation HCV Platelets Plasma blue light 405 nm light |
| url | https://doi.org/10.1038/s41598-024-83171-3 |
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