Effect of lupeol on testicular lesions induced by cadmium chloride in rats
Objective: Heavy metals and environmental pollutants, such as cadmium chloride, congenital disorders, and certain diseases, can lead to infertility in men. In this study, the effects of lupeol (an active pentacyclic triterpenoid with antioxidant properties) on testicular injuries induced by cadmium...
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Mashhad University of Medical Sciences
2025-01-01
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| Series: | Avicenna Journal of Phytomedicine |
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| Online Access: | https://ajp.mums.ac.ir/article_24716_695b2f54c3e6e332e9643e156ef4a208.pdf |
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| author | Parmida Arabkarami Pejman Mortazavi Saeed Hesaraki |
| author_facet | Parmida Arabkarami Pejman Mortazavi Saeed Hesaraki |
| author_sort | Parmida Arabkarami |
| collection | DOAJ |
| description | Objective: Heavy metals and environmental pollutants, such as cadmium chloride, congenital disorders, and certain diseases, can lead to infertility in men. In this study, the effects of lupeol (an active pentacyclic triterpenoid with antioxidant properties) on testicular injuries induced by cadmium chloride were investigated in male rats.Materials and Methods: Lupeol was obtained from Sigma-Aldrich, and the experiment included 40 male Wistar rats divided into 8 groups (healthy control, healthy rats treated with 100, 200, and 400 mg/kg of lupeol, cadmium chloride, and three groups that received cadmium chloride and were treated with 100, 200, and 400 mg/kg of lupeol). After oral treatment, rats were anesthetized, and blood and testicular tissue sampling was done. Subsequent analysis of oxidative stress enzymes, such as malondialdehyde (MDA) and superoxide dismutase (SOD), testosterone, sperm motility, and Aquaporin 9 (AQ9) levels was performed using ELISA, histopathology, and immunohistochemistry techniques, respectively. Immunohistochemistry staining was done for expression of Aquaporin 9 in seminiferous tubules.Results: The results showed that compared to the healthy control, cadmium chloride caused a significant decrease in SOD, testosterone, sperm motility, and sperm vitality, with severe destruction of spermatogenic tubes and a significant increase in MDA and AQ9 rate. Rats which were treated with cadmium chloride+400 mg/kg of lupeol showed a significant increase in SOD, testosterone, histopathology, sperm motility, and sperm vitality rate, and significant organization of spermatogenic tubes in testis tissue. There was also a decrease in MDA and AQ9 of rats that received high-dose lupeol. (P<0.001)Conclusion: This study suggests that lupeol has a high potential for improving male reproduction and antioxidants in rats exposed to oxidative stress. |
| format | Article |
| id | doaj-art-3e5bf2689b2e4e65a54e8af9c695ba74 |
| institution | Kabale University |
| issn | 2228-7930 2228-7949 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj-art-3e5bf2689b2e4e65a54e8af9c695ba742025-08-20T03:52:56ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492025-01-0115190791910.22038/ajp.2024.2471624716Effect of lupeol on testicular lesions induced by cadmium chloride in ratsParmida Arabkarami0Pejman Mortazavi1Saeed Hesaraki2Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranObjective: Heavy metals and environmental pollutants, such as cadmium chloride, congenital disorders, and certain diseases, can lead to infertility in men. In this study, the effects of lupeol (an active pentacyclic triterpenoid with antioxidant properties) on testicular injuries induced by cadmium chloride were investigated in male rats.Materials and Methods: Lupeol was obtained from Sigma-Aldrich, and the experiment included 40 male Wistar rats divided into 8 groups (healthy control, healthy rats treated with 100, 200, and 400 mg/kg of lupeol, cadmium chloride, and three groups that received cadmium chloride and were treated with 100, 200, and 400 mg/kg of lupeol). After oral treatment, rats were anesthetized, and blood and testicular tissue sampling was done. Subsequent analysis of oxidative stress enzymes, such as malondialdehyde (MDA) and superoxide dismutase (SOD), testosterone, sperm motility, and Aquaporin 9 (AQ9) levels was performed using ELISA, histopathology, and immunohistochemistry techniques, respectively. Immunohistochemistry staining was done for expression of Aquaporin 9 in seminiferous tubules.Results: The results showed that compared to the healthy control, cadmium chloride caused a significant decrease in SOD, testosterone, sperm motility, and sperm vitality, with severe destruction of spermatogenic tubes and a significant increase in MDA and AQ9 rate. Rats which were treated with cadmium chloride+400 mg/kg of lupeol showed a significant increase in SOD, testosterone, histopathology, sperm motility, and sperm vitality rate, and significant organization of spermatogenic tubes in testis tissue. There was also a decrease in MDA and AQ9 of rats that received high-dose lupeol. (P<0.001)Conclusion: This study suggests that lupeol has a high potential for improving male reproduction and antioxidants in rats exposed to oxidative stress.https://ajp.mums.ac.ir/article_24716_695b2f54c3e6e332e9643e156ef4a208.pdfinfertilitylupeolcadmium chloridemalondialdehydesuperoxide dismutase |
| spellingShingle | Parmida Arabkarami Pejman Mortazavi Saeed Hesaraki Effect of lupeol on testicular lesions induced by cadmium chloride in rats Avicenna Journal of Phytomedicine infertility lupeol cadmium chloride malondialdehyde superoxide dismutase |
| title | Effect of lupeol on testicular lesions induced by cadmium chloride in rats |
| title_full | Effect of lupeol on testicular lesions induced by cadmium chloride in rats |
| title_fullStr | Effect of lupeol on testicular lesions induced by cadmium chloride in rats |
| title_full_unstemmed | Effect of lupeol on testicular lesions induced by cadmium chloride in rats |
| title_short | Effect of lupeol on testicular lesions induced by cadmium chloride in rats |
| title_sort | effect of lupeol on testicular lesions induced by cadmium chloride in rats |
| topic | infertility lupeol cadmium chloride malondialdehyde superoxide dismutase |
| url | https://ajp.mums.ac.ir/article_24716_695b2f54c3e6e332e9643e156ef4a208.pdf |
| work_keys_str_mv | AT parmidaarabkarami effectoflupeolontesticularlesionsinducedbycadmiumchlorideinrats AT pejmanmortazavi effectoflupeolontesticularlesionsinducedbycadmiumchlorideinrats AT saeedhesaraki effectoflupeolontesticularlesionsinducedbycadmiumchlorideinrats |