State of the immune system in women using the “Provag” probiotic
Lactobacilli are widely used in clinical practice as probiotics, biologically active additives and probiotic products for functional nutrition. Some probiotics can be considered as bacterial vaccines due to induction of immune response, accompanied by production of specific antibodies. The...
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2020-08-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/1572 |
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| author | V. A. Kozlov A. G. Borisov A. A. Savchenko A. E. Kondakov I. V. Kudryavtsev |
| author_facet | V. A. Kozlov A. G. Borisov A. A. Savchenko A. E. Kondakov I. V. Kudryavtsev |
| author_sort | V. A. Kozlov |
| collection | DOAJ |
| description | Lactobacilli are widely used in clinical practice as probiotics, biologically active additives and probiotic products for functional nutrition. Some probiotics can be considered as bacterial vaccines due to induction of immune response, accompanied by production of specific antibodies. The aim of the present study was to evaluate the state of cellular and humoral immunity in women by using probiotic strains of lactobacilli. The study included 31 healthy women aged 25-45 years. As a source of probiotic lactobacterial complex, we used the “Provag” preparation (RU 77.99.11.003.E.003746.02.11 of 11.02.2011, 1 capsule contains 109 Lactobacillus gasseri 57C, Lactobacillus fermentum 57A и Lactobacillus plantarum 57B). The drug was used for 30 days, at a rate of one capsule per day. The immune system was examined twice: before administering the drug and after 30 days of treatment. The study of blood lymphocyte populations and subpopulations was performed by flow cytometry using direct immunofluorescence technique. The concentration of IgA, IgM, IgG in blood serum was determined using enzyme immunoassay. To determine specific antibodies, we used passive hemagglutination reaction with erythrocyte diagnosticum. The complex of probiotic lactobacilli Lactobacillus gasseri, Lactobacillus fermentum and Lactobacillus plantarum corresponding to the “Provag” preparation was used as a source of antigen. It has been revealed that the number of T and B lymphocytes in peripheral blood increased after 30 days of treatment with the probiotic preparation “Provag” in healthy women. Elevated contents of T cells was due to the T helper cell fraction. Increased levels of T helpers and B lymphocytes were associated with stimulation of humoral immunity, as evidenced by increasing concentration of IgA and IgG in blood serum. By means of passive hemagglutination reaction, we have found that 90% of healthy women showed increased concentrations of specific IgA in blood after 30 days of treatment with “Provag” preparation. |
| format | Article |
| id | doaj-art-3e5a50705b844cd5bd8d413776b2fc92 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2020-08-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-3e5a50705b844cd5bd8d413776b2fc922025-08-20T03:37:44ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2020-08-0122478579010.15789/1563-0625-SOT-15721311State of the immune system in women using the “Provag” probioticV. A. Kozlov0A. G. Borisov1A. A. Savchenko2A. E. Kondakov3I. V. Kudryavtsev4Federal State Budgetary Scientific Institution «Scientific Research Institute of Fundamental and Clinical Immunology»Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of medical problems of the North, Krasnoyarsk State Medical University named after Prof. V.F. Voino-YasenetskyFederal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of medical problems of the North, Siberian Federal UniversityFederal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of medical problems of the NorthFederal State Budgetary Scientific Institution "Institute of Experimental Medicine"(FSBSI "IEM"); Pavlov First Saint Petersburg State Medical UniversityLactobacilli are widely used in clinical practice as probiotics, biologically active additives and probiotic products for functional nutrition. Some probiotics can be considered as bacterial vaccines due to induction of immune response, accompanied by production of specific antibodies. The aim of the present study was to evaluate the state of cellular and humoral immunity in women by using probiotic strains of lactobacilli. The study included 31 healthy women aged 25-45 years. As a source of probiotic lactobacterial complex, we used the “Provag” preparation (RU 77.99.11.003.E.003746.02.11 of 11.02.2011, 1 capsule contains 109 Lactobacillus gasseri 57C, Lactobacillus fermentum 57A и Lactobacillus plantarum 57B). The drug was used for 30 days, at a rate of one capsule per day. The immune system was examined twice: before administering the drug and after 30 days of treatment. The study of blood lymphocyte populations and subpopulations was performed by flow cytometry using direct immunofluorescence technique. The concentration of IgA, IgM, IgG in blood serum was determined using enzyme immunoassay. To determine specific antibodies, we used passive hemagglutination reaction with erythrocyte diagnosticum. The complex of probiotic lactobacilli Lactobacillus gasseri, Lactobacillus fermentum and Lactobacillus plantarum corresponding to the “Provag” preparation was used as a source of antigen. It has been revealed that the number of T and B lymphocytes in peripheral blood increased after 30 days of treatment with the probiotic preparation “Provag” in healthy women. Elevated contents of T cells was due to the T helper cell fraction. Increased levels of T helpers and B lymphocytes were associated with stimulation of humoral immunity, as evidenced by increasing concentration of IgA and IgG in blood serum. By means of passive hemagglutination reaction, we have found that 90% of healthy women showed increased concentrations of specific IgA in blood after 30 days of treatment with “Provag” preparation.https://www.mimmun.ru/mimmun/article/view/1572immunityt helperscytotoxic t lymphocytesb lymphocytesimmunoglobulinsprobiotic “provag” |
| spellingShingle | V. A. Kozlov A. G. Borisov A. A. Savchenko A. E. Kondakov I. V. Kudryavtsev State of the immune system in women using the “Provag” probiotic Медицинская иммунология immunity t helpers cytotoxic t lymphocytes b lymphocytes immunoglobulins probiotic “provag” |
| title | State of the immune system in women using the “Provag” probiotic |
| title_full | State of the immune system in women using the “Provag” probiotic |
| title_fullStr | State of the immune system in women using the “Provag” probiotic |
| title_full_unstemmed | State of the immune system in women using the “Provag” probiotic |
| title_short | State of the immune system in women using the “Provag” probiotic |
| title_sort | state of the immune system in women using the provag probiotic |
| topic | immunity t helpers cytotoxic t lymphocytes b lymphocytes immunoglobulins probiotic “provag” |
| url | https://www.mimmun.ru/mimmun/article/view/1572 |
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