The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trial

Abstract Background Non-alcoholic fatty liver disease (NAFLD) is a disease with high complications. An increment in dietary fiber consumption is an approach to NAFLD management, and chitosan as dietary fiber can play a role in the management of NAFLD. Thus, the present study aimed to investigate the...

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Main Authors: Mohammad Ali Mohsenpoor, Karim Parastouei, Maryam Taghdir, Marzieh Akbarzadeh, Sepideh Abbaszadeh, Mohammad Ali Abyazi Heris, Mohammad Reza Mansouri Rad, Mohammad Amin Jafari, Ronak Borzooei
Format: Article
Language:English
Published: BMC 2025-03-01
Series:Journal of Health, Population and Nutrition
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Online Access:https://doi.org/10.1186/s41043-025-00797-3
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author Mohammad Ali Mohsenpoor
Karim Parastouei
Maryam Taghdir
Marzieh Akbarzadeh
Sepideh Abbaszadeh
Mohammad Ali Abyazi Heris
Mohammad Reza Mansouri Rad
Mohammad Amin Jafari
Ronak Borzooei
author_facet Mohammad Ali Mohsenpoor
Karim Parastouei
Maryam Taghdir
Marzieh Akbarzadeh
Sepideh Abbaszadeh
Mohammad Ali Abyazi Heris
Mohammad Reza Mansouri Rad
Mohammad Amin Jafari
Ronak Borzooei
author_sort Mohammad Ali Mohsenpoor
collection DOAJ
description Abstract Background Non-alcoholic fatty liver disease (NAFLD) is a disease with high complications. An increment in dietary fiber consumption is an approach to NAFLD management, and chitosan as dietary fiber can play a role in the management of NAFLD. Thus, the present study aimed to investigate the effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indices in adults with NAFLD. Methods Seventy-two adults with NAFLD were randomly assigned to consume either 1.5 g/day chitosan or placebo along with a low-calorie (− 500 kcal/day) diet for 8 weeks in a parallel, randomized, double-blinded, placebo-controlled, clinical trial. Participants were assessed for dietary intake, physical activity, and anthropometric indices. Blood samples were taken to measure fasting blood sugar (FBS), cholesterol, triglycerides, high- and low-density lipoprotein (HDL and LDL). Liver function indices including alanine aminotransferase (ALT), aspartate transaminase (AST), and gamma-glutamyltransferase (GGT) were evaluated using blood samples as the primary outcomes. Fatty liver index (FLI), hepatic steatosis index (HSI), and triglyceride-glucose index (TyG) were calculated as hepatic steatosis predictors’ indices. Results After 8 weeks of study, 66 participants finished the study. In comparison with placebo, chitosan supplementation reduced weight (P = 0.041), waist circumference (P = 0.049), AST (P = 0.040), ALT (P = 0.001), and GGT (P = 0.028). Although the reduction of FBS, triglycerides, cholesterol, LDL, FLI, HSI, and TyG, and increment in HDL was higher in the chitosan group, the results were not significant (P > 0.05). Conclusions Eight-week supplementation with 1.5 g/day chitosan along with a low-calorie diet could possibly reduce weight, waist circumference, AST, ALT, and GGT, and ameliorate NAFLD. Further investigations are recommended. Trial registration The trial was registered at IRCT.ir as IRCT20140502017522N4 (March 2023).
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spelling doaj-art-3e5a09fd5b044fd0bd1057db3563beff2025-08-20T02:16:34ZengBMCJournal of Health, Population and Nutrition2072-13152025-03-0144111010.1186/s41043-025-00797-3The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trialMohammad Ali Mohsenpoor0Karim Parastouei1Maryam Taghdir2Marzieh Akbarzadeh3Sepideh Abbaszadeh4Mohammad Ali Abyazi Heris5Mohammad Reza Mansouri Rad6Mohammad Amin Jafari7Ronak Borzooei8Student Research Committee, Baqiyatallah University of Medical SciencesHealth Research Centre, Life Style Institute, Baqiyatallah University of Medical SciencesHealth Research Centre, Life Style Institute, Baqiyatallah University of Medical SciencesNutrition Research Center, Department of Community Nutrition, School of Nutrition and Food Sciences, Shiraz University of Medical SciencesHealth Research Centre, Life Style Institute, Baqiyatallah University of Medical SciencesBaqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical SciencesMarine Medicine Research Center, Baqiyatallah University of Medical SciencesStudent Research Committee, Shiraz University of Medical SciencesStudent Research Committee, Baqiyatallah University of Medical SciencesAbstract Background Non-alcoholic fatty liver disease (NAFLD) is a disease with high complications. An increment in dietary fiber consumption is an approach to NAFLD management, and chitosan as dietary fiber can play a role in the management of NAFLD. Thus, the present study aimed to investigate the effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indices in adults with NAFLD. Methods Seventy-two adults with NAFLD were randomly assigned to consume either 1.5 g/day chitosan or placebo along with a low-calorie (− 500 kcal/day) diet for 8 weeks in a parallel, randomized, double-blinded, placebo-controlled, clinical trial. Participants were assessed for dietary intake, physical activity, and anthropometric indices. Blood samples were taken to measure fasting blood sugar (FBS), cholesterol, triglycerides, high- and low-density lipoprotein (HDL and LDL). Liver function indices including alanine aminotransferase (ALT), aspartate transaminase (AST), and gamma-glutamyltransferase (GGT) were evaluated using blood samples as the primary outcomes. Fatty liver index (FLI), hepatic steatosis index (HSI), and triglyceride-glucose index (TyG) were calculated as hepatic steatosis predictors’ indices. Results After 8 weeks of study, 66 participants finished the study. In comparison with placebo, chitosan supplementation reduced weight (P = 0.041), waist circumference (P = 0.049), AST (P = 0.040), ALT (P = 0.001), and GGT (P = 0.028). Although the reduction of FBS, triglycerides, cholesterol, LDL, FLI, HSI, and TyG, and increment in HDL was higher in the chitosan group, the results were not significant (P > 0.05). Conclusions Eight-week supplementation with 1.5 g/day chitosan along with a low-calorie diet could possibly reduce weight, waist circumference, AST, ALT, and GGT, and ameliorate NAFLD. Further investigations are recommended. Trial registration The trial was registered at IRCT.ir as IRCT20140502017522N4 (March 2023).https://doi.org/10.1186/s41043-025-00797-3ChitosanNon-alcoholic fatty liver diseaseLiver function testASTALTGGT
spellingShingle Mohammad Ali Mohsenpoor
Karim Parastouei
Maryam Taghdir
Marzieh Akbarzadeh
Sepideh Abbaszadeh
Mohammad Ali Abyazi Heris
Mohammad Reza Mansouri Rad
Mohammad Amin Jafari
Ronak Borzooei
The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trial
Journal of Health, Population and Nutrition
Chitosan
Non-alcoholic fatty liver disease
Liver function test
AST
ALT
GGT
title The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trial
title_full The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trial
title_fullStr The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trial
title_full_unstemmed The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trial
title_short The effect of chitosan supplementation on liver function, hepatic steatosis predictors, and metabolic indicators in adults with non-alcoholic fatty liver disease: a randomized, double-blinded, placebo-controlled, clinical trial
title_sort effect of chitosan supplementation on liver function hepatic steatosis predictors and metabolic indicators in adults with non alcoholic fatty liver disease a randomized double blinded placebo controlled clinical trial
topic Chitosan
Non-alcoholic fatty liver disease
Liver function test
AST
ALT
GGT
url https://doi.org/10.1186/s41043-025-00797-3
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