Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested Muskmelons
DNA methylation is a crucial epigenetic marker linked to plant defense responses, but its significance in fungal infection of postharvest fruits remains poorly understood. This study indicated that <i>Trichothecium roseum</i> inoculation increased ROS production, enhanced phenylpropanoid...
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MDPI AG
2025-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/11/4/243 |
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| author | Liang Lyu Lei Li Chenglong Zhao Yuchao Ning Yawen Luo Xining He Mina Nan |
| author_facet | Liang Lyu Lei Li Chenglong Zhao Yuchao Ning Yawen Luo Xining He Mina Nan |
| author_sort | Liang Lyu |
| collection | DOAJ |
| description | DNA methylation is a crucial epigenetic marker linked to plant defense responses, but its significance in fungal infection of postharvest fruits remains poorly understood. This study indicated that <i>Trichothecium roseum</i> inoculation increased ROS production, enhanced phenylpropanoid metabolism-related enzyme activity, and promoted lignin accumulation in harvested muskmelon fruits <i>(Cucumis melo</i> cv. Yujinxiang) within 24 h post-inoculation (hpi). In addition, whole-genome bisulfite sequencing showed that genomic DNA methylation levels of muskmelon decreased by 6.15% at 24 hpi. Notably, CG sites exhibited a higher methylation level and the largest number of differentially methylated regions (DMRs). Moreover, 176 DMR-associated genes (DMGs) involved in the defense response, 134 DMGs in the ROS metabolic pathway, and 41 DMGs in phenylpropanoid metabolism were identified. The differentially expressed genes harboring differential methylation were mainly influenced by hypomethylation and exhibited elevated transcript levels, involved in phenylpropanoid biosynthesis and biosynthesis of secondary metabolites. |
| format | Article |
| id | doaj-art-3e51c8137a3f438790d670e28dd3ff05 |
| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-3e51c8137a3f438790d670e28dd3ff052025-08-20T03:13:50ZengMDPI AGJournal of Fungi2309-608X2025-03-0111424310.3390/jof11040243Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested MuskmelonsLiang Lyu0Lei Li1Chenglong Zhao2Yuchao Ning3Yawen Luo4Xining He5Mina Nan6College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaCollege of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, ChinaLaboratory and Practice Base Management Center, Gansu Agricultural University, Lanzhou 730070, ChinaDNA methylation is a crucial epigenetic marker linked to plant defense responses, but its significance in fungal infection of postharvest fruits remains poorly understood. This study indicated that <i>Trichothecium roseum</i> inoculation increased ROS production, enhanced phenylpropanoid metabolism-related enzyme activity, and promoted lignin accumulation in harvested muskmelon fruits <i>(Cucumis melo</i> cv. Yujinxiang) within 24 h post-inoculation (hpi). In addition, whole-genome bisulfite sequencing showed that genomic DNA methylation levels of muskmelon decreased by 6.15% at 24 hpi. Notably, CG sites exhibited a higher methylation level and the largest number of differentially methylated regions (DMRs). Moreover, 176 DMR-associated genes (DMGs) involved in the defense response, 134 DMGs in the ROS metabolic pathway, and 41 DMGs in phenylpropanoid metabolism were identified. The differentially expressed genes harboring differential methylation were mainly influenced by hypomethylation and exhibited elevated transcript levels, involved in phenylpropanoid biosynthesis and biosynthesis of secondary metabolites.https://www.mdpi.com/2309-608X/11/4/243DNA methylationbiotic stressmuskmelonsdefense response |
| spellingShingle | Liang Lyu Lei Li Chenglong Zhao Yuchao Ning Yawen Luo Xining He Mina Nan Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested Muskmelons Journal of Fungi DNA methylation biotic stress muskmelons defense response |
| title | Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested Muskmelons |
| title_full | Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested Muskmelons |
| title_fullStr | Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested Muskmelons |
| title_full_unstemmed | Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested Muskmelons |
| title_short | Whole-Genome DNA Methylation Analysis of Inoculation with <i>Trichothecium roseum</i> in Harvested Muskmelons |
| title_sort | whole genome dna methylation analysis of inoculation with i trichothecium roseum i in harvested muskmelons |
| topic | DNA methylation biotic stress muskmelons defense response |
| url | https://www.mdpi.com/2309-608X/11/4/243 |
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