Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae)
Abstract The bay snook (Petenia splendida) belongs to the family Cichlidae, distributed in the southeastern region of Mexico and Central America, and it is a very important cichlid at a commercial level. Despite their potential for aquaculture, little information exists on their metabolic pathways r...
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Sociedade Brasileira de Ictiologia
2025-04-01
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| Series: | Neotropical Ichthyology |
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| author | Luis Daniel Jiménez-Martínez Gloria Gertrudys Asencio-Alcudia Carlos Alfonso Álvarez-González Alejandra del Carmen Castillo-Collado Vicente Morales-Garcia Carina Shianya Alvarez-Villagomez Candelario Rodríguez-Pérez Rafael Martínez-García César Sepúlveda-Quiroz Graciela María Pérez-Jiménez |
| author_facet | Luis Daniel Jiménez-Martínez Gloria Gertrudys Asencio-Alcudia Carlos Alfonso Álvarez-González Alejandra del Carmen Castillo-Collado Vicente Morales-Garcia Carina Shianya Alvarez-Villagomez Candelario Rodríguez-Pérez Rafael Martínez-García César Sepúlveda-Quiroz Graciela María Pérez-Jiménez |
| author_sort | Luis Daniel Jiménez-Martínez |
| collection | DOAJ |
| description | Abstract The bay snook (Petenia splendida) belongs to the family Cichlidae, distributed in the southeastern region of Mexico and Central America, and it is a very important cichlid at a commercial level. Despite their potential for aquaculture, little information exists on their metabolic pathways related to physiology and nutrition. This study focuses on the expression of the fatty acid desaturases (fads2) and elongases (elovl4b and elovl5) genes in embryos 0 days post-hatching (dph) and larvae of 5, 10, 15, 20, 25, and 30 dph. Additionally, the differential expression of these genes was analyzed in the liver, intestine, kidney, muscle, heart, testes, gills, stomach, pancreas, brain, and adipose tissue in adult fish. Our study utilized specific qPCR primers designed for fads2, elovl4b, and elovl5 to detect the expression of these genes. We found the highest expression were observed in larvae at 20 dph and adults, predominantly in the liver and intestine of the three genes tested. Importantly, our results showed that the desaturase and elongase genes are differentially expressed in the tissues of adults of P. splendida, and their expression fluctuates during larval development. |
| format | Article |
| id | doaj-art-3e4f3cd5996c4aeabe86150fb6cd5c7b |
| institution | Kabale University |
| issn | 1982-0224 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj-art-3e4f3cd5996c4aeabe86150fb6cd5c7b2025-08-20T03:53:01ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-02242025-04-0123110.1590/1982-0224-2024-0095Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae)Luis Daniel Jiménez-Martínezhttps://orcid.org/0000-0001-6304-5669Gloria Gertrudys Asencio-Alcudiahttps://orcid.org/0000-0002-7302-659XCarlos Alfonso Álvarez-Gonzálezhttps://orcid.org/0000-0001-9240-0041Alejandra del Carmen Castillo-Colladohttps://orcid.org/0000-0003-0069-8456Vicente Morales-Garciahttps://orcid.org/0000-0002-7868-2529Carina Shianya Alvarez-Villagomezhttps://orcid.org/0000-0002-1606-9200Candelario Rodríguez-Pérezhttps://orcid.org/0000-0002-8233-0577Rafael Martínez-Garcíahttps://orcid.org/0000-0003-2560-1518César Sepúlveda-Quirozhttps://orcid.org/0000-0003-1111-0275Graciela María Pérez-Jiménezhttps://orcid.org/0000-0002-7842-217XAbstract The bay snook (Petenia splendida) belongs to the family Cichlidae, distributed in the southeastern region of Mexico and Central America, and it is a very important cichlid at a commercial level. Despite their potential for aquaculture, little information exists on their metabolic pathways related to physiology and nutrition. This study focuses on the expression of the fatty acid desaturases (fads2) and elongases (elovl4b and elovl5) genes in embryos 0 days post-hatching (dph) and larvae of 5, 10, 15, 20, 25, and 30 dph. Additionally, the differential expression of these genes was analyzed in the liver, intestine, kidney, muscle, heart, testes, gills, stomach, pancreas, brain, and adipose tissue in adult fish. Our study utilized specific qPCR primers designed for fads2, elovl4b, and elovl5 to detect the expression of these genes. We found the highest expression were observed in larvae at 20 dph and adults, predominantly in the liver and intestine of the three genes tested. Importantly, our results showed that the desaturase and elongase genes are differentially expressed in the tissues of adults of P. splendida, and their expression fluctuates during larval development.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252025000100221&lng=en&tlng=enDesaturaseEarly ontogenyElongasesGene expressionPetenia splendida |
| spellingShingle | Luis Daniel Jiménez-Martínez Gloria Gertrudys Asencio-Alcudia Carlos Alfonso Álvarez-González Alejandra del Carmen Castillo-Collado Vicente Morales-Garcia Carina Shianya Alvarez-Villagomez Candelario Rodríguez-Pérez Rafael Martínez-García César Sepúlveda-Quiroz Graciela María Pérez-Jiménez Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae) Neotropical Ichthyology Desaturase Early ontogeny Elongases Gene expression Petenia splendida |
| title | Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_full | Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_fullStr | Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_full_unstemmed | Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_short | Molecular cloning and gene expression of desaturases and elongases in embryos, larvae, and adults of bay snook Petenia splendida (Cichliformes: Cichlidae) |
| title_sort | molecular cloning and gene expression of desaturases and elongases in embryos larvae and adults of bay snook petenia splendida cichliformes cichlidae |
| topic | Desaturase Early ontogeny Elongases Gene expression Petenia splendida |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252025000100221&lng=en&tlng=en |
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