The Proliferation of Chang Liver Cells After Simulated Microgravity Induction
This study aimed to assess the recovery capability of Chang liver cells (CCL-13) following simulated microgravity (SMG) induction. CCL-13 cells were cultured under SMG conditions for 72 h, and control group cells were cultured under 1G conditions for an identical duration. Cells from the SMG and con...
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MDPI AG
2025-02-01
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| author | Huy Nghia Quang Hoang Chi Nguyen Quynh Ho Loan Thi Tung Dang Nhan Lu Chinh Phan Chung Chinh Doan Han Thai Minh Nguyen Cuong Phan Minh Le Son Nghia Hoang Long Thanh Le |
| author_facet | Huy Nghia Quang Hoang Chi Nguyen Quynh Ho Loan Thi Tung Dang Nhan Lu Chinh Phan Chung Chinh Doan Han Thai Minh Nguyen Cuong Phan Minh Le Son Nghia Hoang Long Thanh Le |
| author_sort | Huy Nghia Quang Hoang |
| collection | DOAJ |
| description | This study aimed to assess the recovery capability of Chang liver cells (CCL-13) following simulated microgravity (SMG) induction. CCL-13 cells were cultured under SMG conditions for 72 h, and control group cells were cultured under 1G conditions for an identical duration. Cells from the SMG and control groups were further cultured under 1G conditions and assessed after 24 h and 72 h intervals in the gravity recovery experiment. The WST1 results indicated that CCL-13 proliferation was more evident in the control group than in the SMG group after both the 24 h and 72 h intervals. The control group had a lower percentage of CCL-13 cells in the G0/G1 phase compared with the SMG group at both time points, and it exhibited a higher total percentage of cells in the S and G2/M phases. The control group exhibited elevated levels of cell-cycle-related proteins, including cyclin A, cyclin D, and cdk6, compared with the SMG group. The flow cytometry results revealed that the apoptotic rate in the control group was significantly lower than that in the SMG group at both the 24 h and 72 h time points. However, the apoptotic percentage in the SMG group at the 72-h mark was significantly lower than that at the 24-h mark. SMG reduces the viability and proliferation ability of CCL-13 cells. After a period of recovery and adaptation to normal gravity conditions (1G), the CCL-13 cells in the SMG group showed better signs of recovery after 72 h than after 24 h. |
| format | Article |
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| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-3e4dbfbd2703491ca334346b9f155b8c2025-08-20T02:42:46ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-02-0147316410.3390/cimb47030164The Proliferation of Chang Liver Cells After Simulated Microgravity InductionHuy Nghia Quang Hoang0Chi Nguyen Quynh Ho1Loan Thi Tung Dang2Nhan Lu Chinh Phan3Chung Chinh Doan4Han Thai Minh Nguyen5Cuong Phan Minh Le6Son Nghia Hoang7Long Thanh Le8Animal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamAnimal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamFaculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh 700000, VietnamAnimal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamAnimal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamAnimal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamAnimal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamAnimal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamAnimal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh 700000, VietnamThis study aimed to assess the recovery capability of Chang liver cells (CCL-13) following simulated microgravity (SMG) induction. CCL-13 cells were cultured under SMG conditions for 72 h, and control group cells were cultured under 1G conditions for an identical duration. Cells from the SMG and control groups were further cultured under 1G conditions and assessed after 24 h and 72 h intervals in the gravity recovery experiment. The WST1 results indicated that CCL-13 proliferation was more evident in the control group than in the SMG group after both the 24 h and 72 h intervals. The control group had a lower percentage of CCL-13 cells in the G0/G1 phase compared with the SMG group at both time points, and it exhibited a higher total percentage of cells in the S and G2/M phases. The control group exhibited elevated levels of cell-cycle-related proteins, including cyclin A, cyclin D, and cdk6, compared with the SMG group. The flow cytometry results revealed that the apoptotic rate in the control group was significantly lower than that in the SMG group at both the 24 h and 72 h time points. However, the apoptotic percentage in the SMG group at the 72-h mark was significantly lower than that at the 24-h mark. SMG reduces the viability and proliferation ability of CCL-13 cells. After a period of recovery and adaptation to normal gravity conditions (1G), the CCL-13 cells in the SMG group showed better signs of recovery after 72 h than after 24 h.https://www.mdpi.com/1467-3045/47/3/164Chang liver cellssimulated microgravityrecovery after simulated microgravity inductionproliferationcell cycleviability |
| spellingShingle | Huy Nghia Quang Hoang Chi Nguyen Quynh Ho Loan Thi Tung Dang Nhan Lu Chinh Phan Chung Chinh Doan Han Thai Minh Nguyen Cuong Phan Minh Le Son Nghia Hoang Long Thanh Le The Proliferation of Chang Liver Cells After Simulated Microgravity Induction Current Issues in Molecular Biology Chang liver cells simulated microgravity recovery after simulated microgravity induction proliferation cell cycle viability |
| title | The Proliferation of Chang Liver Cells After Simulated Microgravity Induction |
| title_full | The Proliferation of Chang Liver Cells After Simulated Microgravity Induction |
| title_fullStr | The Proliferation of Chang Liver Cells After Simulated Microgravity Induction |
| title_full_unstemmed | The Proliferation of Chang Liver Cells After Simulated Microgravity Induction |
| title_short | The Proliferation of Chang Liver Cells After Simulated Microgravity Induction |
| title_sort | proliferation of chang liver cells after simulated microgravity induction |
| topic | Chang liver cells simulated microgravity recovery after simulated microgravity induction proliferation cell cycle viability |
| url | https://www.mdpi.com/1467-3045/47/3/164 |
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