Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites
The rational design of synthetic peptides is proposed as an efficient strategy for the structural investigation of crucial protein domains difficult to be produced. Only after half a century since the function of ACE was first reported, was its crystal structure solved. The main obstacle to be overc...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2010/820476 |
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| author | Zinovia Spyranti Athanassios S. Galanis George Pairas Georgios A. Spyroulias Evy Manessi-Zoupa Paul Cordopatis |
| author_facet | Zinovia Spyranti Athanassios S. Galanis George Pairas Georgios A. Spyroulias Evy Manessi-Zoupa Paul Cordopatis |
| author_sort | Zinovia Spyranti |
| collection | DOAJ |
| description | The rational design of synthetic peptides is proposed as an efficient strategy for the structural investigation of crucial protein domains difficult to be produced. Only after half a century since the function of ACE was first reported, was its crystal structure solved. The main obstacle to be overcome for the determination of the high resolution structure was the crystallization of the highly hydrophobic transmembrane domain. Following our previous work, synthetic peptides and Zinc(II) metal ions are used to build structural maquettes of the two Zn-catalytic active sites of the ACE somatic isoform. Structural investigations of the synthetic peptides, representing the two different somatic isoform active sites, through circular dichroism and NMR experiments are reported. |
| format | Article |
| id | doaj-art-3e4cfa4849994a2bbb3ef6ab5d58848e |
| institution | OA Journals |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-3e4cfa4849994a2bbb3ef6ab5d58848e2025-08-20T02:16:39ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2010-01-01201010.1155/2010/820476820476Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic SitesZinovia Spyranti0Athanassios S. Galanis1George Pairas2Georgios A. Spyroulias3Evy Manessi-Zoupa4Paul Cordopatis5Department of Pharmacy, University of Patras, GR-26504, Patras, GreeceDepartment of Pharmacy, University of Patras, GR-26504, Patras, GreeceDepartment of Pharmacy, University of Patras, GR-26504, Patras, GreeceDepartment of Pharmacy, University of Patras, GR-26504, Patras, GreeceDepartment of Chemistry, University of Patras, GR-26504, Patras, GreeceDepartment of Pharmacy, University of Patras, GR-26504, Patras, GreeceThe rational design of synthetic peptides is proposed as an efficient strategy for the structural investigation of crucial protein domains difficult to be produced. Only after half a century since the function of ACE was first reported, was its crystal structure solved. The main obstacle to be overcome for the determination of the high resolution structure was the crystallization of the highly hydrophobic transmembrane domain. Following our previous work, synthetic peptides and Zinc(II) metal ions are used to build structural maquettes of the two Zn-catalytic active sites of the ACE somatic isoform. Structural investigations of the synthetic peptides, representing the two different somatic isoform active sites, through circular dichroism and NMR experiments are reported.http://dx.doi.org/10.1155/2010/820476 |
| spellingShingle | Zinovia Spyranti Athanassios S. Galanis George Pairas Georgios A. Spyroulias Evy Manessi-Zoupa Paul Cordopatis Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites Bioinorganic Chemistry and Applications |
| title | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
| title_full | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
| title_fullStr | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
| title_full_unstemmed | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
| title_short | Synthetic Peptides as Structural Maquettes of Angiotensin-I Converting Enzyme Catalytic Sites |
| title_sort | synthetic peptides as structural maquettes of angiotensin i converting enzyme catalytic sites |
| url | http://dx.doi.org/10.1155/2010/820476 |
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