Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild Rice

【Objective】High-temperature and rainy weather lead to pre-harvest sprouting (PHS), severely impacting yield and quality traits of rice. Identification and screening of germplasm or genetic resources is a fundamental pathway for developing new varieties resistant to PHS and eliminating PHS damage.【Me...

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Main Authors: Jiaxiao HU, Jin LIU, Xiaoding MA, Hang TU, Huiying ZHOU, Bingxin MENG, Di CUI, Maomao LI, Longzhi HAN, Liqin YU
Format: Article
Language:English
Published: Guangdong Academy of Agricultural Sciences 2024-11-01
Series:Guangdong nongye kexue
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Online Access:http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202411006
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author Jiaxiao HU
Jin LIU
Xiaoding MA
Hang TU
Huiying ZHOU
Bingxin MENG
Di CUI
Maomao LI
Longzhi HAN
Liqin YU
author_facet Jiaxiao HU
Jin LIU
Xiaoding MA
Hang TU
Huiying ZHOU
Bingxin MENG
Di CUI
Maomao LI
Longzhi HAN
Liqin YU
author_sort Jiaxiao HU
collection DOAJ
description 【Objective】High-temperature and rainy weather lead to pre-harvest sprouting (PHS), severely impacting yield and quality traits of rice. Identification and screening of germplasm or genetic resources is a fundamental pathway for developing new varieties resistant to PHS and eliminating PHS damage.【Method】In the study, a set of chromosome segment substitution lines (CSSLs) derived from Dongxiang wild rice ('C35') as the donor parent and 'Nipponbare' ('NIP') as the recipient parent were used as the experimental materials, and then PHS resistance were evaluated and QTLs were mapped in 2021-2023, with an aim to screen PHS germplasm and identify major QTLs.【Result】Dongxiang wild rice 'C35' exhibited strong dormancy under different environments with a pre-harvest sprouting rate (PHSR) of 0.00%; 'Nipponbare' showed significant PHS with an average PHSR of 31.95%. The PHSR varied widely among CSSL populations, the phenotypic repeatability of PHSR was relatively high in different years, and ten lines from the CSSL populations with strong dormancy and resistance to PHS were screened. A total of 14 QTLs controlling the PHSR were detected, and four QTLs were repeatedly detected under different environments. These QTLs formed four QTL clusters (qPHSRC1, qPHSRC2, qPHSRC8 and qPHSRC9), among which qPHSRC2 and qPHSRC9 had higher LOD values, phenotypic contribution rate (%) and additive effect, and qPHSRC2 was a newly discovered major QTL cluster.【Conclusion】A batch of PHS resistant germplasms were screened and 14 QTLs were mapped, four QTL clusters were repeatedly identified, and a new major QTL cluster qPHSRC2 controlling the PHSR was discovered.
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publisher Guangdong Academy of Agricultural Sciences
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series Guangdong nongye kexue
spelling doaj-art-3e45a109524f4c99a863f8a6c505a3f22025-01-18T06:57:00ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2024-11-015111616810.16768/j.issn.1004-874X.2024.11.006202411006Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild RiceJiaxiao HU0Jin LIU1Xiaoding MA2Hang TU3Huiying ZHOU4Bingxin MENG5Di CUI6Maomao LI7Longzhi HAN8Liqin YU9Rice Research Institute, Jiangxi Academy of Agricultural Sciences/Research Center of Jiangxi Crop Germplasm Resources, Nanchang 330200, ChinaRice Research Institute, Jiangxi Academy of Agricultural Sciences/Research Center of Jiangxi Crop Germplasm Resources, Nanchang 330200, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRice Research Institute, Jiangxi Academy of Agricultural Sciences/Research Center of Jiangxi Crop Germplasm Resources, Nanchang 330200, ChinaRice Research Institute, Jiangxi Academy of Agricultural Sciences/Research Center of Jiangxi Crop Germplasm Resources, Nanchang 330200, ChinaRice Research Institute, Jiangxi Academy of Agricultural Sciences/Research Center of Jiangxi Crop Germplasm Resources, Nanchang 330200, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRice Research Institute, Jiangxi Academy of Agricultural Sciences/Research Center of Jiangxi Crop Germplasm Resources, Nanchang 330200, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaRice Research Institute, Jiangxi Academy of Agricultural Sciences/Research Center of Jiangxi Crop Germplasm Resources, Nanchang 330200, China【Objective】High-temperature and rainy weather lead to pre-harvest sprouting (PHS), severely impacting yield and quality traits of rice. Identification and screening of germplasm or genetic resources is a fundamental pathway for developing new varieties resistant to PHS and eliminating PHS damage.【Method】In the study, a set of chromosome segment substitution lines (CSSLs) derived from Dongxiang wild rice ('C35') as the donor parent and 'Nipponbare' ('NIP') as the recipient parent were used as the experimental materials, and then PHS resistance were evaluated and QTLs were mapped in 2021-2023, with an aim to screen PHS germplasm and identify major QTLs.【Result】Dongxiang wild rice 'C35' exhibited strong dormancy under different environments with a pre-harvest sprouting rate (PHSR) of 0.00%; 'Nipponbare' showed significant PHS with an average PHSR of 31.95%. The PHSR varied widely among CSSL populations, the phenotypic repeatability of PHSR was relatively high in different years, and ten lines from the CSSL populations with strong dormancy and resistance to PHS were screened. A total of 14 QTLs controlling the PHSR were detected, and four QTLs were repeatedly detected under different environments. These QTLs formed four QTL clusters (qPHSRC1, qPHSRC2, qPHSRC8 and qPHSRC9), among which qPHSRC2 and qPHSRC9 had higher LOD values, phenotypic contribution rate (%) and additive effect, and qPHSRC2 was a newly discovered major QTL cluster.【Conclusion】A batch of PHS resistant germplasms were screened and 14 QTLs were mapped, four QTL clusters were repeatedly identified, and a new major QTL cluster qPHSRC2 controlling the PHSR was discovered.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202411006different years and environmentsdongxiang common wild rice (oryza rufipogon griff.)csslsphs resistanceqtl mapping
spellingShingle Jiaxiao HU
Jin LIU
Xiaoding MA
Hang TU
Huiying ZHOU
Bingxin MENG
Di CUI
Maomao LI
Longzhi HAN
Liqin YU
Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild Rice
Guangdong nongye kexue
different years and environments
dongxiang common wild rice (oryza rufipogon griff.)
cssls
phs resistance
qtl mapping
title Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild Rice
title_full Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild Rice
title_fullStr Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild Rice
title_full_unstemmed Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild Rice
title_short Identification of QTL for Pre-harvest Sprouting Resistance by Using Chromosome Segment Substitution Lines of Dongxiang Common Wild Rice
title_sort identification of qtl for pre harvest sprouting resistance by using chromosome segment substitution lines of dongxiang common wild rice
topic different years and environments
dongxiang common wild rice (oryza rufipogon griff.)
cssls
phs resistance
qtl mapping
url http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202411006
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