Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius)
To investigate into the role of leptin in body mass in high-fat-fed animals. Male striped field mice (Apodemus agrarius) fed high-fat diets were given leptin (0.5 μg/g.d) via intraperitoneal injection for 28 days. Their body mass, digestive metrics, and physiological parameters of food consumption a...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-08-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2025.1592317/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849330645282586624 |
|---|---|
| author | Yue Ren Yue Ren Guangtong Guo Yu Hou Yu Hou Kuiyou Chen Xinsheng Pu Mengfan Tao Yunlong Ren Xin’gen Yang Xin’gen Yang |
| author_facet | Yue Ren Yue Ren Guangtong Guo Yu Hou Yu Hou Kuiyou Chen Xinsheng Pu Mengfan Tao Yunlong Ren Xin’gen Yang Xin’gen Yang |
| author_sort | Yue Ren |
| collection | DOAJ |
| description | To investigate into the role of leptin in body mass in high-fat-fed animals. Male striped field mice (Apodemus agrarius) fed high-fat diets were given leptin (0.5 μg/g.d) via intraperitoneal injection for 28 days. Their body mass, digestive metrics, and physiological parameters of food consumption and energy metabolism were compared to those of the control and high-fat food groups. Firstly, the high-fat diet did not cause weight gain in Apodemus agrarius, and the animals on the diet ate less and had higher apparent digestibility. Furthermore, exogenous leptin injection in A. agrarius reduced food intake, increased fecal content, and reduced apparent digestibility. Additionally, exogenous leptin injection inhibited the activity of the AMPK in the hypothalamus, increased the activity of malonyl CoA, inhibited the expression of orexigenic neuropeptide mRNA, promoted the expression of anorexigenic neuropeptide mRNA, and thus reduced food intake and body mass. Finally, exogenous leptin injection increased uncoupling protein 1 content, T45′-deiodinase II activity, and cytochrome C oxidase activity in brown adipose tissue, increased serum triiodothyronine, and increased animal energy consumption. In conclusion, our data indicate that leptin affects body mass in animals on a high-fat diet in two ways: by inhibiting food intake and increasing energy expenditure. |
| format | Article |
| id | doaj-art-3e2e174dc3684f7a8a5047b6cb568099 |
| institution | Kabale University |
| issn | 1664-042X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj-art-3e2e174dc3684f7a8a5047b6cb5680992025-08-20T03:46:50ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2025-08-011610.3389/fphys.2025.15923171592317Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius)Yue Ren0Yue Ren1Guangtong Guo2Yu Hou3Yu Hou4Kuiyou Chen5Xinsheng Pu6Mengfan Tao7Yunlong Ren8Xin’gen Yang9Xin’gen Yang10College of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaShanxi Key Laboratory of Integrated Pest Management in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaShanxi Key Laboratory of Integrated Pest Management in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaShanxi Key Laboratory of Integrated Pest Management in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taiyuan, ChinaTo investigate into the role of leptin in body mass in high-fat-fed animals. Male striped field mice (Apodemus agrarius) fed high-fat diets were given leptin (0.5 μg/g.d) via intraperitoneal injection for 28 days. Their body mass, digestive metrics, and physiological parameters of food consumption and energy metabolism were compared to those of the control and high-fat food groups. Firstly, the high-fat diet did not cause weight gain in Apodemus agrarius, and the animals on the diet ate less and had higher apparent digestibility. Furthermore, exogenous leptin injection in A. agrarius reduced food intake, increased fecal content, and reduced apparent digestibility. Additionally, exogenous leptin injection inhibited the activity of the AMPK in the hypothalamus, increased the activity of malonyl CoA, inhibited the expression of orexigenic neuropeptide mRNA, promoted the expression of anorexigenic neuropeptide mRNA, and thus reduced food intake and body mass. Finally, exogenous leptin injection increased uncoupling protein 1 content, T45′-deiodinase II activity, and cytochrome C oxidase activity in brown adipose tissue, increased serum triiodothyronine, and increased animal energy consumption. In conclusion, our data indicate that leptin affects body mass in animals on a high-fat diet in two ways: by inhibiting food intake and increasing energy expenditure.https://www.frontiersin.org/articles/10.3389/fphys.2025.1592317/fullApodemus agrariusleptinbody masshigh-fat foodAMPK |
| spellingShingle | Yue Ren Yue Ren Guangtong Guo Yu Hou Yu Hou Kuiyou Chen Xinsheng Pu Mengfan Tao Yunlong Ren Xin’gen Yang Xin’gen Yang Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius) Frontiers in Physiology Apodemus agrarius leptin body mass high-fat food AMPK |
| title | Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius) |
| title_full | Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius) |
| title_fullStr | Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius) |
| title_full_unstemmed | Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius) |
| title_short | Leptin’s crucial modulatory role in regulating body mass homeostasis of high-fat-fed striped field mice (Apodemus agrarius) |
| title_sort | leptin s crucial modulatory role in regulating body mass homeostasis of high fat fed striped field mice apodemus agrarius |
| topic | Apodemus agrarius leptin body mass high-fat food AMPK |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2025.1592317/full |
| work_keys_str_mv | AT yueren leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT yueren leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT guangtongguo leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT yuhou leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT yuhou leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT kuiyouchen leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT xinshengpu leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT mengfantao leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT yunlongren leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT xingenyang leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius AT xingenyang leptinscrucialmodulatoryroleinregulatingbodymasshomeostasisofhighfatfedstripedfieldmiceapodemusagrarius |