Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay.
The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were exa...
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199974&type=printable |
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| author | Yuli Melisa Sierra-Arguello Thales Quedi Furian Gustavo Perdoncini Hamilton L S Moraes Carlos T P Salle Laura B Rodrigues Luciana Ruschel Dos Santos Marcos José Pereira Gomes Vladimir Pinheiro do Nascimento |
| author_facet | Yuli Melisa Sierra-Arguello Thales Quedi Furian Gustavo Perdoncini Hamilton L S Moraes Carlos T P Salle Laura B Rodrigues Luciana Ruschel Dos Santos Marcos José Pereira Gomes Vladimir Pinheiro do Nascimento |
| author_sort | Yuli Melisa Sierra-Arguello |
| collection | DOAJ |
| description | The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were examined for ciprofloxacin, norfloxacin, and nalidixic acid resistance using the minimal inhibitory concentration (MIC) method. Thereafter, the isolates were analyzed by PCR-Restriction Fragment Length Polymorphism (RFLP) assay for detection of Thr-86 mutation. Finally, DNA sequencing was performed for confirmation of gyrA gene mutation. A complete correlation was observed between MICs, PCR-RFLP assay, and sequencing. The results revealed high quinolone resistance rates for C. jejuni (100%) and C. coli (100%) isolates obtained from poultry and moderate resistance for C. jejuni (9.1%) and C. coli (40%) samples of human origin. A mutation in codon 86 of the gyrA gene with a Thr-to-Ile substitution is reported to be the main cause of high resistance to quinolones. This mutation can be analyzed by PCR-RFLP assay, which has been proven to be a simple and fast method for the detection of fluoroquinolone resistance in Campylobacter spp. |
| format | Article |
| id | doaj-art-3e2da11cc71e42e2b1c99bd5c497de28 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-3e2da11cc71e42e2b1c99bd5c497de282025-08-20T02:45:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e019997410.1371/journal.pone.0199974Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay.Yuli Melisa Sierra-ArguelloThales Quedi FurianGustavo PerdonciniHamilton L S MoraesCarlos T P SalleLaura B RodriguesLuciana Ruschel Dos SantosMarcos José Pereira GomesVladimir Pinheiro do NascimentoThe objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one Campylobacter jejuni isolates (30 of poultry origin and 11 of human origin) and 11 Campylobacter coli isolates (10 of human origin and 1 of poultry origin) were examined for ciprofloxacin, norfloxacin, and nalidixic acid resistance using the minimal inhibitory concentration (MIC) method. Thereafter, the isolates were analyzed by PCR-Restriction Fragment Length Polymorphism (RFLP) assay for detection of Thr-86 mutation. Finally, DNA sequencing was performed for confirmation of gyrA gene mutation. A complete correlation was observed between MICs, PCR-RFLP assay, and sequencing. The results revealed high quinolone resistance rates for C. jejuni (100%) and C. coli (100%) isolates obtained from poultry and moderate resistance for C. jejuni (9.1%) and C. coli (40%) samples of human origin. A mutation in codon 86 of the gyrA gene with a Thr-to-Ile substitution is reported to be the main cause of high resistance to quinolones. This mutation can be analyzed by PCR-RFLP assay, which has been proven to be a simple and fast method for the detection of fluoroquinolone resistance in Campylobacter spp.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199974&type=printable |
| spellingShingle | Yuli Melisa Sierra-Arguello Thales Quedi Furian Gustavo Perdoncini Hamilton L S Moraes Carlos T P Salle Laura B Rodrigues Luciana Ruschel Dos Santos Marcos José Pereira Gomes Vladimir Pinheiro do Nascimento Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay. PLoS ONE |
| title | Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay. |
| title_full | Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay. |
| title_fullStr | Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay. |
| title_full_unstemmed | Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay. |
| title_short | Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay. |
| title_sort | fluoroquinolone resistance in campylobacter jejuni and campylobacter coli from poultry and human samples assessed by pcr restriction fragment length polymorphism assay |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199974&type=printable |
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