Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolism
ABSTRACT: Trans: palmitoleic acid (TPA), a naturally occurring trans fatty acid found in ruminant-derived products such as dairy products, has been associated with various potential health benefits. However, its digestion, absorption, tissue distribution, and metabolic properties following oral adm...
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Elsevier
2025-06-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030225001699 |
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| author | Meng Wei Zeqiang Zhou Kequn Lin Zeyuan Deng Jing Li |
| author_facet | Meng Wei Zeqiang Zhou Kequn Lin Zeyuan Deng Jing Li |
| author_sort | Meng Wei |
| collection | DOAJ |
| description | ABSTRACT: Trans: palmitoleic acid (TPA), a naturally occurring trans fatty acid found in ruminant-derived products such as dairy products, has been associated with various potential health benefits. However, its digestion, absorption, tissue distribution, and metabolic properties following oral administration remain insufficiently understood. Here, we conducted pharmacokinetic analyses in C57BL/6J mice to evaluate the absorption, tissue distribution, and metabolism of TPA following oral administration. Our data showed that the plasma concentration of TPA peaked at 9.1 µg·mL−1 at 15 min postadministration, with a terminal elimination half-life of 201.1 min. Moreover, TPA was efficiently distributed to the heart, lung, liver, kidney, brain, and adipose tissue, reaching peak concentrations within 30 to 60 min. These results indicate that TPA is rapidly digested, absorbed, and distributed across multiple tissues in mice and exhibits slow metabolic clearance and an extended residence time in vivo. Within 0 to 480 min following oral administration, TPA underwent bioconversion to trans-vaccenic acid (TVA) and cis-9, trans-11 conjugated linoleic acid (9c11t-CLA), both of which are recognized for their extensive health benefits. The bioconversion rates were 71.57% to 72.44% for TVA and 44.05% to 53.23% for 9c11t-CLA. Furthermore, TPA significantly reduced triglyceride and total cholesterol levels in hepatocytes with steatosis. Notably, inhibiting the bioconversion of TPA to TVA and 9c11t-CLA did not impair its ability to reduce lipid accumulation in hepatocytes, suggesting that the beneficial effects of TPA on lipid metabolism are independent of its bioconversion to TVA and 9c11t-CLA. This study provides a reference for dairy fat intake and establishes a foundation for further exploration of the physiological effects of TPA. |
| format | Article |
| id | doaj-art-3e2a4fa4125547ada4c73760e7a889e7 |
| institution | Kabale University |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Journal of Dairy Science |
| spelling | doaj-art-3e2a4fa4125547ada4c73760e7a889e72025-08-20T03:47:31ZengElsevierJournal of Dairy Science0022-03022025-06-0110865502551510.3168/jds.2024-25913Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolismMeng Wei0Zeqiang Zhou1Kequn Lin2Zeyuan Deng3Jing Li4State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, Jiangxi, China 330047; College of Food science and Technology, Nanchang University, Nanchang, Jiangxi, China 330031State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, Jiangxi, China 330047; College of Food science and Technology, Nanchang University, Nanchang, Jiangxi, China 330031State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, Jiangxi, China 330047; College of Food science and Technology, Nanchang University, Nanchang, Jiangxi, China 330031State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, Jiangxi, China 330047; College of Food science and Technology, Nanchang University, Nanchang, Jiangxi, China 330031; International Institute of Food Innovation, Nanchang University, Nanchang, Jiangxi, China 330200State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, Jiangxi, China 330047; College of Food science and Technology, Nanchang University, Nanchang, Jiangxi, China 330031; International Institute of Food Innovation, Nanchang University, Nanchang, Jiangxi, China 330200; Corresponding authorABSTRACT: Trans: palmitoleic acid (TPA), a naturally occurring trans fatty acid found in ruminant-derived products such as dairy products, has been associated with various potential health benefits. However, its digestion, absorption, tissue distribution, and metabolic properties following oral administration remain insufficiently understood. Here, we conducted pharmacokinetic analyses in C57BL/6J mice to evaluate the absorption, tissue distribution, and metabolism of TPA following oral administration. Our data showed that the plasma concentration of TPA peaked at 9.1 µg·mL−1 at 15 min postadministration, with a terminal elimination half-life of 201.1 min. Moreover, TPA was efficiently distributed to the heart, lung, liver, kidney, brain, and adipose tissue, reaching peak concentrations within 30 to 60 min. These results indicate that TPA is rapidly digested, absorbed, and distributed across multiple tissues in mice and exhibits slow metabolic clearance and an extended residence time in vivo. Within 0 to 480 min following oral administration, TPA underwent bioconversion to trans-vaccenic acid (TVA) and cis-9, trans-11 conjugated linoleic acid (9c11t-CLA), both of which are recognized for their extensive health benefits. The bioconversion rates were 71.57% to 72.44% for TVA and 44.05% to 53.23% for 9c11t-CLA. Furthermore, TPA significantly reduced triglyceride and total cholesterol levels in hepatocytes with steatosis. Notably, inhibiting the bioconversion of TPA to TVA and 9c11t-CLA did not impair its ability to reduce lipid accumulation in hepatocytes, suggesting that the beneficial effects of TPA on lipid metabolism are independent of its bioconversion to TVA and 9c11t-CLA. This study provides a reference for dairy fat intake and establishes a foundation for further exploration of the physiological effects of TPA.http://www.sciencedirect.com/science/article/pii/S0022030225001699trans-palmitoleic acidpharmacokineticsbioconversionlipid metabolism |
| spellingShingle | Meng Wei Zeqiang Zhou Kequn Lin Zeyuan Deng Jing Li Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolism Journal of Dairy Science trans-palmitoleic acid pharmacokinetics bioconversion lipid metabolism |
| title | Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolism |
| title_full | Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolism |
| title_fullStr | Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolism |
| title_full_unstemmed | Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolism |
| title_short | Absorption, metabolism, and bioconversion of trans-palmitoleic acid in C57BL/6J mice: Implications for lipid metabolism |
| title_sort | absorption metabolism and bioconversion of trans palmitoleic acid in c57bl 6j mice implications for lipid metabolism |
| topic | trans-palmitoleic acid pharmacokinetics bioconversion lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0022030225001699 |
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