Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternative

Plant-based alternatives are considered microbiologically safe; however, recent studies have raised concerns about hygienic quality. Additionally, the relationship between microbiological safety and polyphenolic content in fermented products remains unexplored. This study assessed the potential of f...

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Main Authors: Pamela Canaviri-Paz, Thamani Freedom Gondo, Anna Kjellström, Tawanda Mandoga, Jaison Sithole, Elin Oscarsson, Margareta Sandahl, Åsa Håkansson
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525001415
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author Pamela Canaviri-Paz
Thamani Freedom Gondo
Anna Kjellström
Tawanda Mandoga
Jaison Sithole
Elin Oscarsson
Margareta Sandahl
Åsa Håkansson
author_facet Pamela Canaviri-Paz
Thamani Freedom Gondo
Anna Kjellström
Tawanda Mandoga
Jaison Sithole
Elin Oscarsson
Margareta Sandahl
Åsa Håkansson
author_sort Pamela Canaviri-Paz
collection DOAJ
description Plant-based alternatives are considered microbiologically safe; however, recent studies have raised concerns about hygienic quality. Additionally, the relationship between microbiological safety and polyphenolic content in fermented products remains unexplored. This study assessed the potential of four autochthonous Lactiplantibacillus plantarum strains (3, 5, 9, and 10) to impact microbial composition and modulate polyphenol and saponin levels in a quinoa-based beverage. The results identified Lactiplantibacillus plantarum strains 3, 9, and 10 as effective in inhibiting Enterobacteriaceae (p = 0.001), and increasing concentrations of glycosylated flavonoids, 3-phenyllactic acid, and saponins. However, Lactiplantibacillus plantarum 10 demonstrated a decrease in saponin levels, whereas Lactiplantibacillus plantarum 5 increased the abundance of aglycones, highlighting strain-specific differences. Notably, principal component analysis revealed less differences between inoculated samples and control, indicating potential contribution of the native microbiota to the fermentation. This study enhances the understanding of interactions between starter cultures, native microbiota, and bioactive compounds in plant-based fermented beverages.
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publishDate 2025-02-01
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spelling doaj-art-3e27f2fb5f0e4baa994347e12eb18d1d2025-08-20T02:04:15ZengElsevierFood Chemistry: X2590-15752025-02-012610229410.1016/j.fochx.2025.102294Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternativePamela Canaviri-Paz0Thamani Freedom Gondo1Anna Kjellström2Tawanda Mandoga3Jaison Sithole4Elin Oscarsson5Margareta Sandahl6Åsa Håkansson7Department of Process and Life Science Engineering, Faculty of Engineering, LTH, Lund University. Box 124, SE-221 00 Lund, SwedenCentre for Analysis and Synthesis, Department of Chemistry, Faculty of Science, Lund University. Box 124, 221 00 Lund, Sweden; Correspondence to Thamani Freedom Gondo, Centre for Analysis and Synthesis, Department of Chemistry, Faculty of Science, Lund University, Box 124, 221 00 Lund, Sweden.Department of Process and Life Science Engineering, Faculty of Engineering, LTH, Lund University. Box 124, SE-221 00 Lund, SwedenDepartment of Process and Life Science Engineering, Faculty of Engineering, LTH, Lund University. Box 124, SE-221 00 Lund, SwedenDepartment of Process and Life Science Engineering, Faculty of Engineering, LTH, Lund University. Box 124, SE-221 00 Lund, SwedenDepartment of Process and Life Science Engineering, Faculty of Engineering, LTH, Lund University. Box 124, SE-221 00 Lund, SwedenCentre for Analysis and Synthesis, Department of Chemistry, Faculty of Science, Lund University. Box 124, 221 00 Lund, SwedenDepartment of Process and Life Science Engineering, Faculty of Engineering, LTH, Lund University. Box 124, SE-221 00 Lund, SwedenPlant-based alternatives are considered microbiologically safe; however, recent studies have raised concerns about hygienic quality. Additionally, the relationship between microbiological safety and polyphenolic content in fermented products remains unexplored. This study assessed the potential of four autochthonous Lactiplantibacillus plantarum strains (3, 5, 9, and 10) to impact microbial composition and modulate polyphenol and saponin levels in a quinoa-based beverage. The results identified Lactiplantibacillus plantarum strains 3, 9, and 10 as effective in inhibiting Enterobacteriaceae (p = 0.001), and increasing concentrations of glycosylated flavonoids, 3-phenyllactic acid, and saponins. However, Lactiplantibacillus plantarum 10 demonstrated a decrease in saponin levels, whereas Lactiplantibacillus plantarum 5 increased the abundance of aglycones, highlighting strain-specific differences. Notably, principal component analysis revealed less differences between inoculated samples and control, indicating potential contribution of the native microbiota to the fermentation. This study enhances the understanding of interactions between starter cultures, native microbiota, and bioactive compounds in plant-based fermented beverages.http://www.sciencedirect.com/science/article/pii/S2590157525001415Starter culturesNative microbiotaEnterobacteriaceaePhenolic compoundsSaponinsHigh-resolution mass spectrometry
spellingShingle Pamela Canaviri-Paz
Thamani Freedom Gondo
Anna Kjellström
Tawanda Mandoga
Jaison Sithole
Elin Oscarsson
Margareta Sandahl
Åsa Håkansson
Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternative
Food Chemistry: X
Starter cultures
Native microbiota
Enterobacteriaceae
Phenolic compounds
Saponins
High-resolution mass spectrometry
title Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternative
title_full Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternative
title_fullStr Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternative
title_full_unstemmed Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternative
title_short Influence of autochthonous Lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa-based beverage as a non-dairy alternative
title_sort influence of autochthonous lactiplantibacillus plantarum strains on microbial safety and bioactive compounds in a fermented quinoa based beverage as a non dairy alternative
topic Starter cultures
Native microbiota
Enterobacteriaceae
Phenolic compounds
Saponins
High-resolution mass spectrometry
url http://www.sciencedirect.com/science/article/pii/S2590157525001415
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