Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study
Abstract Background It is questionable whether or not a short period of deep anesthesia can have long lasting effects on immune suppression. Methods To analyze specific effects of deep anesthesia on immune modulation, a randomized-controlled, single-blinded study, monocentric, pilot-study was conduc...
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BMC
2025-03-01
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| Series: | BMC Anesthesiology |
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| Online Access: | https://doi.org/10.1186/s12871-025-02980-9 |
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| author | Xuan Duc Nguyen Audrey Horn Dania Fischer Grietje Beck Cora C. Spannenberger Brice Gaudilliere Jean-Louis Horn Hermann-Josef Thierse Thomas Frietsch |
| author_facet | Xuan Duc Nguyen Audrey Horn Dania Fischer Grietje Beck Cora C. Spannenberger Brice Gaudilliere Jean-Louis Horn Hermann-Josef Thierse Thomas Frietsch |
| author_sort | Xuan Duc Nguyen |
| collection | DOAJ |
| description | Abstract Background It is questionable whether or not a short period of deep anesthesia can have long lasting effects on immune suppression. Methods To analyze specific effects of deep anesthesia on immune modulation, a randomized-controlled, single-blinded study, monocentric, pilot-study was conducted at a level 1 orthopedic and trauma center. Inclusion criteria were patients scheduled for extended shoulder surgery with an ASA score between 1 to 3 (n = 186). Patients on immune modulating drugs or with immune deficits were excluded. The remaining patients were enrolled and randomized to either deep or light anesthesia (n = 18). Patient were randomized to receive either deep anesthesia or light anesthesia for 60 min or longer. The primary aim of the study was to compare cellular activity of T-cells, NK-cells and monocytes after anesthesia. Phagocytosis and cellular lysis activity of neutrophils and monocytes were analyzed by flow cytometry. Secondly, we analyzed anesthesia induced protein expresssion pattern in human monocytes by a standardized proteomic approach, implicating quantitative two-dimensional (2D) differential gel electrophoresis and Delta2D software analyses coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and Mascot analysis. Results Anesthesia duration was 109 min in the deep anesthesia group with 81 ± 17 min of BIS < 45 and a mean BIS of 38 ± 14. The light anesthesia group received anesthesia for 111 min with 13 ± 8 min of BIS < 45 and a mean BIS 56 ± 8. Cytotoxic T-cells decreased fivefold in the light anesthesia group compared to the deep anesthesia group (-28 ± 13% vs. -6 ± 18%, respectively). The number of NK-cells (p = 0.0127) and regulatory T-cells (p = 0.0217) both dropped after deep anesthesia to almost half of the plasma level. Phagocytosis activity of neutrophils and monocytes was constant with a 67% decreased trend of intracellular lysis in monocytes (p = 0.0625). Quantitative proteomic analyses revealed 27 anesthesia-regulated protein spots in human monocytes, 14 of which were significantly identified by MALDI-MS, and were related to processes such as macrophage function and lymphocyte proliferation, tumor progression and apoptosis. Conclusions Deep anesthesia inhibited immune competent defense cells (killer cells and regulatory T-cells) and had a general suppression on the phagocytic function of all circulating immune competent cells. Trial registration Clinicaltrial.gov identifier: NCT02794896. |
| format | Article |
| id | doaj-art-3e14eb397fe34f6eaedbcd4c5bd1a664 |
| institution | Kabale University |
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| publishDate | 2025-03-01 |
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| series | BMC Anesthesiology |
| spelling | doaj-art-3e14eb397fe34f6eaedbcd4c5bd1a6642025-08-20T03:41:50ZengBMCBMC Anesthesiology1471-22532025-03-0125111810.1186/s12871-025-02980-9Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot studyXuan Duc Nguyen0Audrey Horn1Dania Fischer2Grietje Beck3Cora C. Spannenberger4Brice Gaudilliere5Jean-Louis Horn6Hermann-Josef Thierse7Thomas Frietsch8Amita Lab, Department of ScienceDepartment of Anesthesiology Perioperative and Pain Medicine, Stanford UniversityDepartment Anesthesiology, Ruprecht Karls University HeidelbergFaculty University Medicine Mannheim, Anesthesia and Critical Care Medicine, Ruprecht Karls University HeidelbergTÜV, Occupational Health Services GmbhDepartment of Anesthesiology Perioperative and Pain Medicine, Stanford UniversityDepartment of Anesthesiology Perioperative and Pain Medicine, Stanford UniversityLaboratory for Immunology & Proteomics, Department of Dermatology and University Medical Center Mannheim, University of HeidelbergFaculty University Medicine Mannheim, Anesthesia and Critical Care Medicine, Ruprecht Karls University HeidelbergAbstract Background It is questionable whether or not a short period of deep anesthesia can have long lasting effects on immune suppression. Methods To analyze specific effects of deep anesthesia on immune modulation, a randomized-controlled, single-blinded study, monocentric, pilot-study was conducted at a level 1 orthopedic and trauma center. Inclusion criteria were patients scheduled for extended shoulder surgery with an ASA score between 1 to 3 (n = 186). Patients on immune modulating drugs or with immune deficits were excluded. The remaining patients were enrolled and randomized to either deep or light anesthesia (n = 18). Patient were randomized to receive either deep anesthesia or light anesthesia for 60 min or longer. The primary aim of the study was to compare cellular activity of T-cells, NK-cells and monocytes after anesthesia. Phagocytosis and cellular lysis activity of neutrophils and monocytes were analyzed by flow cytometry. Secondly, we analyzed anesthesia induced protein expresssion pattern in human monocytes by a standardized proteomic approach, implicating quantitative two-dimensional (2D) differential gel electrophoresis and Delta2D software analyses coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and Mascot analysis. Results Anesthesia duration was 109 min in the deep anesthesia group with 81 ± 17 min of BIS < 45 and a mean BIS of 38 ± 14. The light anesthesia group received anesthesia for 111 min with 13 ± 8 min of BIS < 45 and a mean BIS 56 ± 8. Cytotoxic T-cells decreased fivefold in the light anesthesia group compared to the deep anesthesia group (-28 ± 13% vs. -6 ± 18%, respectively). The number of NK-cells (p = 0.0127) and regulatory T-cells (p = 0.0217) both dropped after deep anesthesia to almost half of the plasma level. Phagocytosis activity of neutrophils and monocytes was constant with a 67% decreased trend of intracellular lysis in monocytes (p = 0.0625). Quantitative proteomic analyses revealed 27 anesthesia-regulated protein spots in human monocytes, 14 of which were significantly identified by MALDI-MS, and were related to processes such as macrophage function and lymphocyte proliferation, tumor progression and apoptosis. Conclusions Deep anesthesia inhibited immune competent defense cells (killer cells and regulatory T-cells) and had a general suppression on the phagocytic function of all circulating immune competent cells. Trial registration Clinicaltrial.gov identifier: NCT02794896.https://doi.org/10.1186/s12871-025-02980-9Cellular immune responseAnesthesia depthLymphocyte proliferationMonocyte proteomeNK-cells |
| spellingShingle | Xuan Duc Nguyen Audrey Horn Dania Fischer Grietje Beck Cora C. Spannenberger Brice Gaudilliere Jean-Louis Horn Hermann-Josef Thierse Thomas Frietsch Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study BMC Anesthesiology Cellular immune response Anesthesia depth Lymphocyte proliferation Monocyte proteome NK-cells |
| title | Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study |
| title_full | Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study |
| title_fullStr | Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study |
| title_full_unstemmed | Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study |
| title_short | Suppressive effects of deep balanced anesthesia on cellular immunity and protein expression: a randomized-controlled pilot study |
| title_sort | suppressive effects of deep balanced anesthesia on cellular immunity and protein expression a randomized controlled pilot study |
| topic | Cellular immune response Anesthesia depth Lymphocyte proliferation Monocyte proteome NK-cells |
| url | https://doi.org/10.1186/s12871-025-02980-9 |
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