MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathway
Introduction and Objectives: Hepatocellular carcinoma (HCC), a leading cause of cancer fatalities, challenges clinicians with high recurrence and metastasis rates, urging the need for novel prognostic markers and therapeutic avenues. Minichromosome maintenance complex component 3 (MCM3) has been imp...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Annals of Hepatology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268125000092 |
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| author | Wei-Guo Tang Jin-Feng Feng Xian Li Qi-Man Sun Jin-Wu Hu Xiao-Lu Ma Yan-Yan Nie Yang Xu Jian Sun Qi-Meng Chang |
| author_facet | Wei-Guo Tang Jin-Feng Feng Xian Li Qi-Man Sun Jin-Wu Hu Xiao-Lu Ma Yan-Yan Nie Yang Xu Jian Sun Qi-Meng Chang |
| author_sort | Wei-Guo Tang |
| collection | DOAJ |
| description | Introduction and Objectives: Hepatocellular carcinoma (HCC), a leading cause of cancer fatalities, challenges clinicians with high recurrence and metastasis rates, urging the need for novel prognostic markers and therapeutic avenues. Minichromosome maintenance complex component 3 (MCM3) has been implicated in various cancers, but its role in HCC is not well-characterized. Materials and Methods: We investigated MCM3 expression in HCC through cell line and patient sample analyses, functional assays to determine its effect on cellular behaviors, and signal pathway exploration. Results: Elevated MCM3 expression was identified in both HCC cell lines and patient tissues, correlating with microvascular invasion, advanced cancer stage, and reduced survival. Functionally, MCM3 fueled HCC cellular proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in vitro and expedited tumor growth in vivo. Mechanistically, MCM3 was found to potentiate EMT by upregulating Twist via the AKT signaling pathway. Conclusions: MCM3 emerges as an oncogenic influencer in HCC, driving disease progression through the AKT/Twist axis. Its expression patterns hold prognostic value, and targeting MCM3 may offer a novel therapeutic strategy for HCC. |
| format | Article |
| id | doaj-art-3e12b60bb4c0429086781035cde80961 |
| institution | DOAJ |
| issn | 1665-2681 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj-art-3e12b60bb4c0429086781035cde809612025-08-20T03:20:21ZengElsevierAnnals of Hepatology1665-26812025-01-0130110178510.1016/j.aohep.2025.101785MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathwayWei-Guo Tang0Jin-Feng Feng1Xian Li2Qi-Man Sun3Jin-Wu Hu4Xiao-Lu Ma5Yan-Yan Nie6Yang Xu7Jian Sun8Qi-Meng Chang9Department of Hepatobiliary and Pancreatic Surgery, Minhang Hospital, Fudan University, Shanghai 201199, China; Key laboratory of whole-period monitoring and precise intervention of digestive cancer (SMHC), Minhang Hospital, Fudan University, Shanghai 201199, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, Minhang Hospital, Fudan University, Shanghai 201199, China; Key laboratory of whole-period monitoring and precise intervention of digestive cancer (SMHC), Minhang Hospital, Fudan University, Shanghai 201199, ChinaDepartment of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, PR ChinaDepartment of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, PR ChinaDepartment of Liver Cancer, Shanghai Geriatrics Medical Center, 2560 Chunshen Road, Shanghai 201104, ChinaDepartment of Clinical Laboratory, Shanghai Cancer Center, Fudan University, Shanghai, PR ChinaShanghai Laboratory Animal Research Center, Shanghai 201203, ChinaDepartment of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, PR ChinaDepartment of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, PR China; Corresponding authors.Department of Hepatobiliary and Pancreatic Surgery, Minhang Hospital, Fudan University, Shanghai 201199, China; Key laboratory of whole-period monitoring and precise intervention of digestive cancer (SMHC), Minhang Hospital, Fudan University, Shanghai 201199, China; Corresponding authors.Introduction and Objectives: Hepatocellular carcinoma (HCC), a leading cause of cancer fatalities, challenges clinicians with high recurrence and metastasis rates, urging the need for novel prognostic markers and therapeutic avenues. Minichromosome maintenance complex component 3 (MCM3) has been implicated in various cancers, but its role in HCC is not well-characterized. Materials and Methods: We investigated MCM3 expression in HCC through cell line and patient sample analyses, functional assays to determine its effect on cellular behaviors, and signal pathway exploration. Results: Elevated MCM3 expression was identified in both HCC cell lines and patient tissues, correlating with microvascular invasion, advanced cancer stage, and reduced survival. Functionally, MCM3 fueled HCC cellular proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in vitro and expedited tumor growth in vivo. Mechanistically, MCM3 was found to potentiate EMT by upregulating Twist via the AKT signaling pathway. Conclusions: MCM3 emerges as an oncogenic influencer in HCC, driving disease progression through the AKT/Twist axis. Its expression patterns hold prognostic value, and targeting MCM3 may offer a novel therapeutic strategy for HCC.http://www.sciencedirect.com/science/article/pii/S1665268125000092Hepatocellular carcinomaEpithelial–mesenchymal transitionPrognostic biomarkerMCM3AKT/Twist pathway |
| spellingShingle | Wei-Guo Tang Jin-Feng Feng Xian Li Qi-Man Sun Jin-Wu Hu Xiao-Lu Ma Yan-Yan Nie Yang Xu Jian Sun Qi-Meng Chang MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathway Annals of Hepatology Hepatocellular carcinoma Epithelial–mesenchymal transition Prognostic biomarker MCM3 AKT/Twist pathway |
| title | MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathway |
| title_full | MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathway |
| title_fullStr | MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathway |
| title_full_unstemmed | MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathway |
| title_short | MCM3 promotes hepatocellular carcinoma progression via Epithelial-mesenchymal Transition through AKT/Twist signaling pathway |
| title_sort | mcm3 promotes hepatocellular carcinoma progression via epithelial mesenchymal transition through akt twist signaling pathway |
| topic | Hepatocellular carcinoma Epithelial–mesenchymal transition Prognostic biomarker MCM3 AKT/Twist pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1665268125000092 |
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