Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers
Potato tuber greening occurs due to the chlorophyll accumulation upon exposure to light, however, fundamental information on tuber chlorophyll metabolism is lacking. We measured the effect of varying light exposure (0, 48, 96, and 168 h) on chlorophyll concentration and gene expression of enzymes in...
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Academy of Sciences of the Czech Republic, Institute of Experimental Botany
2025-03-01
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| Series: | Photosynthetica |
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| Online Access: | https://ps.ueb.cas.cz/artkey/phs-202501-0005_relative-importance-of-chlorophyll-metabolic-genes-for-light-induced-greening-of-potato-tubers.php |
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| author | S. TANIOS T. THANGAVEL A. EYLES R.S. TEGG C.R. WILSON |
| author_facet | S. TANIOS T. THANGAVEL A. EYLES R.S. TEGG C.R. WILSON |
| author_sort | S. TANIOS |
| collection | DOAJ |
| description | Potato tuber greening occurs due to the chlorophyll accumulation upon exposure to light, however, fundamental information on tuber chlorophyll metabolism is lacking. We measured the effect of varying light exposure (0, 48, 96, and 168 h) on chlorophyll concentration and gene expression of enzymes in the chlorophyll metabolic pathway in the potato varieties that differ in greening propensity. Greening was associated with the upregulation of genes involved in chlorophyll biosynthesis, particularly glutamyl-tRNA reductase 1, magnesium-chelatase subunit H, and magnesium-protoporphyrin IX monomethyl ester cyclase, and downregulation of genes involved in chlorophyll cycling and degradation, including chlorophyllide a oxygenase, and pheophorbide a oxygenase. Our findings suggest that relative resistance to tuber greening propensity may be due to a weaker upregulation of chlorophyll biosynthesis genes and weaker downregulation of chlorophyll degradation genes that occurs in susceptible varieties. The association of these biosynthesis and degradation genes with greening susceptibility may provide possible breeding targets for the future development of more greening-resistant varieties. |
| format | Article |
| id | doaj-art-3dee2bea01f94bcba97e3d46aa31cb33 |
| institution | DOAJ |
| issn | 0300-3604 1573-9058 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Academy of Sciences of the Czech Republic, Institute of Experimental Botany |
| record_format | Article |
| series | Photosynthetica |
| spelling | doaj-art-3dee2bea01f94bcba97e3d46aa31cb332025-08-20T02:49:44ZengAcademy of Sciences of the Czech Republic, Institute of Experimental BotanyPhotosynthetica0300-36041573-90582025-03-01631374510.32615/ps.2025.003phs-202501-0005Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubersS. TANIOS0T. THANGAVEL1A. EYLES2R.S. TEGG3C.R. WILSON4Tasmanian Institute of Agriculture, University of Tasmania, New Town Research Laboratories, 13 St. Johns Avenue, New Town, Tasmania 7008, AustraliaTasmanian Institute of Agriculture, University of Tasmania, New Town Research Laboratories, 13 St. Johns Avenue, New Town, Tasmania 7008, AustraliaTasmanian Institute of Agriculture, University of Tasmania, New Town Research Laboratories, 13 St. Johns Avenue, New Town, Tasmania 7008, AustraliaTasmanian Institute of Agriculture, University of Tasmania, New Town Research Laboratories, 13 St. Johns Avenue, New Town, Tasmania 7008, AustraliaTasmanian Institute of Agriculture, University of Tasmania, New Town Research Laboratories, 13 St. Johns Avenue, New Town, Tasmania 7008, AustraliaPotato tuber greening occurs due to the chlorophyll accumulation upon exposure to light, however, fundamental information on tuber chlorophyll metabolism is lacking. We measured the effect of varying light exposure (0, 48, 96, and 168 h) on chlorophyll concentration and gene expression of enzymes in the chlorophyll metabolic pathway in the potato varieties that differ in greening propensity. Greening was associated with the upregulation of genes involved in chlorophyll biosynthesis, particularly glutamyl-tRNA reductase 1, magnesium-chelatase subunit H, and magnesium-protoporphyrin IX monomethyl ester cyclase, and downregulation of genes involved in chlorophyll cycling and degradation, including chlorophyllide a oxygenase, and pheophorbide a oxygenase. Our findings suggest that relative resistance to tuber greening propensity may be due to a weaker upregulation of chlorophyll biosynthesis genes and weaker downregulation of chlorophyll degradation genes that occurs in susceptible varieties. The association of these biosynthesis and degradation genes with greening susceptibility may provide possible breeding targets for the future development of more greening-resistant varieties.https://ps.ueb.cas.cz/artkey/phs-202501-0005_relative-importance-of-chlorophyll-metabolic-genes-for-light-induced-greening-of-potato-tubers.phpchlorophyll biosynthesischlorophyll degradationchlorophyll metabolic pathwaytuber greening |
| spellingShingle | S. TANIOS T. THANGAVEL A. EYLES R.S. TEGG C.R. WILSON Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers Photosynthetica chlorophyll biosynthesis chlorophyll degradation chlorophyll metabolic pathway tuber greening |
| title | Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers |
| title_full | Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers |
| title_fullStr | Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers |
| title_full_unstemmed | Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers |
| title_short | Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers |
| title_sort | relative importance of chlorophyll metabolic genes for light induced greening of potato tubers |
| topic | chlorophyll biosynthesis chlorophyll degradation chlorophyll metabolic pathway tuber greening |
| url | https://ps.ueb.cas.cz/artkey/phs-202501-0005_relative-importance-of-chlorophyll-metabolic-genes-for-light-induced-greening-of-potato-tubers.php |
| work_keys_str_mv | AT stanios relativeimportanceofchlorophyllmetabolicgenesforlightinducedgreeningofpotatotubers AT tthangavel relativeimportanceofchlorophyllmetabolicgenesforlightinducedgreeningofpotatotubers AT aeyles relativeimportanceofchlorophyllmetabolicgenesforlightinducedgreeningofpotatotubers AT rstegg relativeimportanceofchlorophyllmetabolicgenesforlightinducedgreeningofpotatotubers AT crwilson relativeimportanceofchlorophyllmetabolicgenesforlightinducedgreeningofpotatotubers |