Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue Attachment
<i>Background: Aeromonas hydrophila</i> is a key pathogen affecting freshwater fish, including <i>Labeo rohita</i> (rohu), causing significant aquaculture losses. This study explores the role of intimin and invasin, known virulence factors, in <i>A. hydrophila</i>...
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2025-02-01
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| author | Agradip Bhattacharyya Goutam Banerjee Pritam Chattopadhyay |
| author_facet | Agradip Bhattacharyya Goutam Banerjee Pritam Chattopadhyay |
| author_sort | Agradip Bhattacharyya |
| collection | DOAJ |
| description | <i>Background: Aeromonas hydrophila</i> is a key pathogen affecting freshwater fish, including <i>Labeo rohita</i> (rohu), causing significant aquaculture losses. This study explores the role of intimin and invasin, known virulence factors, in <i>A. hydrophila</i> pathogenesis using in silico methods. <i>Methods</i>: We analyzed the distribution of invasin and intimin across 53 <i>A. hydrophila</i> genomes and examined their physicochemical properties, secondary structures, and 3D models. Since crystal structures were unavailable, homology-based modeling was employed to study the structure of rohu β-integrins. In silico docking was performed to explore the interactions between intimin/invasin and β-integrins. <i>Results</i>: Our findings revealed that intimin and invasin were present in only 6 out of the 53 <i>A. hydrophila</i> strains examined, which were designated as hypervirulent strains. The transmembrane regions of intimin and invasin were modeled as β-barrels, a common feature of porins. The in silico docking experiments indicated the significant binding affinity of invasin and intimin with all the β-integrins of rohu fish, suggesting a critical role in host attachment and cellular internalization. <i>Conclusions</i>: This in silico study highlights the pivotal role of invasin and intimin in host tissue’s binding efficacy, offering valuable insights into the binding potential of <i>A. hydrophila</i> across various organs in rohu fish. |
| format | Article |
| id | doaj-art-3dea257b5d964fb3bb2907bc23fe5c10 |
| institution | OA Journals |
| issn | 2674-1334 |
| language | English |
| publishDate | 2025-02-01 |
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| series | Bacteria |
| spelling | doaj-art-3dea257b5d964fb3bb2907bc23fe5c102025-08-20T02:11:04ZengMDPI AGBacteria2674-13342025-02-0141710.3390/bacteria4010007Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue AttachmentAgradip Bhattacharyya0Goutam Banerjee1Pritam Chattopadhyay2Raja Rammohun Roy Mahavidyalaya, Radhanagar, Nangulpara, Hooghly, West Bengal 712406, IndiaDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USAM.U.C. Women’s College, Burdwan, Purba-Bardhaman, West Bengal 713104, India<i>Background: Aeromonas hydrophila</i> is a key pathogen affecting freshwater fish, including <i>Labeo rohita</i> (rohu), causing significant aquaculture losses. This study explores the role of intimin and invasin, known virulence factors, in <i>A. hydrophila</i> pathogenesis using in silico methods. <i>Methods</i>: We analyzed the distribution of invasin and intimin across 53 <i>A. hydrophila</i> genomes and examined their physicochemical properties, secondary structures, and 3D models. Since crystal structures were unavailable, homology-based modeling was employed to study the structure of rohu β-integrins. In silico docking was performed to explore the interactions between intimin/invasin and β-integrins. <i>Results</i>: Our findings revealed that intimin and invasin were present in only 6 out of the 53 <i>A. hydrophila</i> strains examined, which were designated as hypervirulent strains. The transmembrane regions of intimin and invasin were modeled as β-barrels, a common feature of porins. The in silico docking experiments indicated the significant binding affinity of invasin and intimin with all the β-integrins of rohu fish, suggesting a critical role in host attachment and cellular internalization. <i>Conclusions</i>: This in silico study highlights the pivotal role of invasin and intimin in host tissue’s binding efficacy, offering valuable insights into the binding potential of <i>A. hydrophila</i> across various organs in rohu fish.https://www.mdpi.com/2674-1334/4/1/7<i>A. hydrophila</i>invasinintiminhomology modeling?-integrinsdocking |
| spellingShingle | Agradip Bhattacharyya Goutam Banerjee Pritam Chattopadhyay Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue Attachment Bacteria <i>A. hydrophila</i> invasin intimin homology modeling ?-integrins docking |
| title | Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue Attachment |
| title_full | Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue Attachment |
| title_fullStr | Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue Attachment |
| title_full_unstemmed | Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue Attachment |
| title_short | Investigating the Distribution of Intimin and Invasin in <i>Aeromonas hydrophila</i> and Their Role in Host Tissue Attachment |
| title_sort | investigating the distribution of intimin and invasin in i aeromonas hydrophila i and their role in host tissue attachment |
| topic | <i>A. hydrophila</i> invasin intimin homology modeling ?-integrins docking |
| url | https://www.mdpi.com/2674-1334/4/1/7 |
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