Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)

ABSTRACT Climbing perch, Anabas testudineus, were fed four diets: D0, D500, D1000 and D1500, containing 0, 500, 1000 and 1500 mg of vitamin C per kg of basal diet, respectively, over 70 days. After the growth trial, significantly higher final weight, percent body weight (%WG) and specific growth rat...

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Main Authors: Tanwi Dey, Md. Sakhawat Hossain, Md. Mostufa Kamal, Piash Kumer Ghosh, Md. Shahab Uddin, Md. Tariqul Alam, Sohel Mian, Md. Jakiul Islam
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Aquaculture, Fish and Fisheries
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Online Access:https://doi.org/10.1002/aff2.70048
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author Tanwi Dey
Md. Sakhawat Hossain
Md. Mostufa Kamal
Piash Kumer Ghosh
Md. Shahab Uddin
Md. Tariqul Alam
Sohel Mian
Md. Jakiul Islam
author_facet Tanwi Dey
Md. Sakhawat Hossain
Md. Mostufa Kamal
Piash Kumer Ghosh
Md. Shahab Uddin
Md. Tariqul Alam
Sohel Mian
Md. Jakiul Islam
author_sort Tanwi Dey
collection DOAJ
description ABSTRACT Climbing perch, Anabas testudineus, were fed four diets: D0, D500, D1000 and D1500, containing 0, 500, 1000 and 1500 mg of vitamin C per kg of basal diet, respectively, over 70 days. After the growth trial, significantly higher final weight, percent body weight (%WG) and specific growth rate (SGR) were observed in fish fed D1000 followed by D500 and D0 diets. However, D1500 diet‐fed fish had a significantly lower growth performance among all diet groups. The best feed conversion ratio and protein efficiency ratio were observed in fish fed D1000, followed by D500, with no significant difference between D0 and D1500 groups. Blood parameters were significantly influenced by vitamin C dosages. Fish fed on the D1000 diet exhibited significantly higher white blood cell (WBC), monocyte (MONO) and granulocyte (GRA) counts among all dietary groups. Vitamin C supplementation significantly increased platelet count in the D1000 group. Haemoglobin, haematocrit and red blood cell (RBC) counts showed an increasing trend with higher vitamin C levels, with the highest values in fish fed D1500 followed by D1000 diet. Total immunoglobulin (Ig) content was significantly higher in fish fed D1000 and D1500 diets compared to others, while peroxidase and catalase activity remained unaffected by the vitamin C levels. Vitamin C supplementation enhanced the salinity stress tolerance of fish, with the highest lethal time of 50% mortality (LT50) observed in the D1000 group, while other groups showed intermediate values. The polynomial regression analysis of %WG, LT50, WBC and Ig revealed that the optimum vitamin C supplementation levels were 773.75, 866.67, 934.71 and 1062.5 mg/kg diet, respectively, for climbing perch; these values are also in line with most of the growth and health performance parameters of the fish.
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series Aquaculture, Fish and Fisheries
spelling doaj-art-3de2429db5364d229f70e2b2c9fe92572025-08-20T02:19:10ZengWileyAquaculture, Fish and Fisheries2693-88472025-04-0152n/an/a10.1002/aff2.70048Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)Tanwi Dey0Md. Sakhawat Hossain1Md. Mostufa Kamal2Piash Kumer Ghosh3Md. Shahab Uddin4Md. Tariqul Alam5Sohel Mian6Md. Jakiul Islam7Department of Aquaculture, Faculty of Fisheries Sylhet Agricultural University Sylhet BangladeshDepartment of Aquaculture, Faculty of Fisheries Sylhet Agricultural University Sylhet BangladeshDepartment of Aquaculture, Faculty of Fisheries Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine, Faculty of Veterinary, Animal and Biomedical Science Sylhet Agricultural University Sylhet BangladeshDepartment of Aquaculture, Faculty of Fisheries Sylhet Agricultural University Sylhet BangladeshDepartment of Aquaculture, Faculty of Fisheries Sylhet Agricultural University Sylhet BangladeshDepartment of Fish Biology and Genetics, Faculty of Fisheries Sylhet Agricultural University Sylhet BangladeshDepartment of Fisheries Technology and Quality Control, Faculty of Fisheries Sylhet Agricultural University Sylhet BangladeshABSTRACT Climbing perch, Anabas testudineus, were fed four diets: D0, D500, D1000 and D1500, containing 0, 500, 1000 and 1500 mg of vitamin C per kg of basal diet, respectively, over 70 days. After the growth trial, significantly higher final weight, percent body weight (%WG) and specific growth rate (SGR) were observed in fish fed D1000 followed by D500 and D0 diets. However, D1500 diet‐fed fish had a significantly lower growth performance among all diet groups. The best feed conversion ratio and protein efficiency ratio were observed in fish fed D1000, followed by D500, with no significant difference between D0 and D1500 groups. Blood parameters were significantly influenced by vitamin C dosages. Fish fed on the D1000 diet exhibited significantly higher white blood cell (WBC), monocyte (MONO) and granulocyte (GRA) counts among all dietary groups. Vitamin C supplementation significantly increased platelet count in the D1000 group. Haemoglobin, haematocrit and red blood cell (RBC) counts showed an increasing trend with higher vitamin C levels, with the highest values in fish fed D1500 followed by D1000 diet. Total immunoglobulin (Ig) content was significantly higher in fish fed D1000 and D1500 diets compared to others, while peroxidase and catalase activity remained unaffected by the vitamin C levels. Vitamin C supplementation enhanced the salinity stress tolerance of fish, with the highest lethal time of 50% mortality (LT50) observed in the D1000 group, while other groups showed intermediate values. The polynomial regression analysis of %WG, LT50, WBC and Ig revealed that the optimum vitamin C supplementation levels were 773.75, 866.67, 934.71 and 1062.5 mg/kg diet, respectively, for climbing perch; these values are also in line with most of the growth and health performance parameters of the fish.https://doi.org/10.1002/aff2.70048ascorbic acidblood parametersclimbing perchgrowthstress tolerance
spellingShingle Tanwi Dey
Md. Sakhawat Hossain
Md. Mostufa Kamal
Piash Kumer Ghosh
Md. Shahab Uddin
Md. Tariqul Alam
Sohel Mian
Md. Jakiul Islam
Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)
Aquaculture, Fish and Fisheries
ascorbic acid
blood parameters
climbing perch
growth
stress tolerance
title Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)
title_full Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)
title_fullStr Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)
title_full_unstemmed Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)
title_short Impact of Vitamin C on Growth, Haemato‐Immunological Characteristics and Stress Tolerance Capability of Climbing Perch, Anabas testudineus (Bloch, 1792)
title_sort impact of vitamin c on growth haemato immunological characteristics and stress tolerance capability of climbing perch anabas testudineus bloch 1792
topic ascorbic acid
blood parameters
climbing perch
growth
stress tolerance
url https://doi.org/10.1002/aff2.70048
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