Integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds

Abstract This study aimed to integrate transcriptomes to reveal the regulatory molecular mechanisms of meat quality in different black goat breeds. A comparison of Guizhou Black Goats (GBG♂, n = 7), Black Nubian Goats (NBG♂, n = 7), and their F1 hybrids (FBG♂, GB♀ × NB♂, n = 7) revealed that the FBG...

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Main Authors: Yong Long, Naifeng Zhang, Yanliang Bi, Tao Ma, Pramote Paengkoum, Wen Xiao, Yanpin Zhao, Chao Yuan, Defeng Wang, Yang Yang, Chaozhi Su, Yong Han
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:npj Science of Food
Online Access:https://doi.org/10.1038/s41538-025-00476-x
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author Yong Long
Naifeng Zhang
Yanliang Bi
Tao Ma
Pramote Paengkoum
Wen Xiao
Yanpin Zhao
Chao Yuan
Defeng Wang
Yang Yang
Chaozhi Su
Yong Han
author_facet Yong Long
Naifeng Zhang
Yanliang Bi
Tao Ma
Pramote Paengkoum
Wen Xiao
Yanpin Zhao
Chao Yuan
Defeng Wang
Yang Yang
Chaozhi Su
Yong Han
author_sort Yong Long
collection DOAJ
description Abstract This study aimed to integrate transcriptomes to reveal the regulatory molecular mechanisms of meat quality in different black goat breeds. A comparison of Guizhou Black Goats (GBG♂, n = 7), Black Nubian Goats (NBG♂, n = 7), and their F1 hybrids (FBG♂, GB♀ × NB♂, n = 7) revealed that the FBG demonstrated notable improvements in meat quality parameters, muscle fiber traits, fatty acid and amino acid compositions, mineral content, intramuscular fat (IMF), and inosine monophosphate (IMP) levels. Moreover, transcriptome analysis identified 119, 77, and 82 differentially expressed genes (DEGs) in the GBG vs NBG, FBG vs GBG, and FBG vs NBG comparisons, respectively. Among these, 111, 74, and 82 DEGs were associated with IMF deposition, and 61, 34, and 32 DEGs were linked to IMP deposition, respectively. Of particular note, the analysis pinpointed key genes related to IMF (PFKM and FZD4) and IMP (AMPD3, ENPP1, ENTPD1, ENTPD8, and PRPS2), laying a solid data groundwork for future research aimed at enhancing the meat quality of black goats.
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issn 2396-8370
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spelling doaj-art-3dd9b218cffe42968b4fef32aadc8d542025-08-20T04:02:42ZengNature Portfolionpj Science of Food2396-83702025-07-019111410.1038/s41538-025-00476-xIntegrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breedsYong Long0Naifeng Zhang1Yanliang Bi2Tao Ma3Pramote Paengkoum4Wen Xiao5Yanpin Zhao6Chao Yuan7Defeng Wang8Yang Yang9Chaozhi Su10Yong Han11Guizhou University of Traditional Chinese MedicineInstitute of Feed Research of Chinese Academy of Agricultural ScienceInstitute of Feed Research of Chinese Academy of Agricultural ScienceInstitute of Feed Research of Chinese Academy of Agricultural ScienceSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of TechnologyInstitute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Sciences, Guizhou Academy of Agricultural SciencesGuizhou University of Traditional Chinese MedicineAbstract This study aimed to integrate transcriptomes to reveal the regulatory molecular mechanisms of meat quality in different black goat breeds. A comparison of Guizhou Black Goats (GBG♂, n = 7), Black Nubian Goats (NBG♂, n = 7), and their F1 hybrids (FBG♂, GB♀ × NB♂, n = 7) revealed that the FBG demonstrated notable improvements in meat quality parameters, muscle fiber traits, fatty acid and amino acid compositions, mineral content, intramuscular fat (IMF), and inosine monophosphate (IMP) levels. Moreover, transcriptome analysis identified 119, 77, and 82 differentially expressed genes (DEGs) in the GBG vs NBG, FBG vs GBG, and FBG vs NBG comparisons, respectively. Among these, 111, 74, and 82 DEGs were associated with IMF deposition, and 61, 34, and 32 DEGs were linked to IMP deposition, respectively. Of particular note, the analysis pinpointed key genes related to IMF (PFKM and FZD4) and IMP (AMPD3, ENPP1, ENTPD1, ENTPD8, and PRPS2), laying a solid data groundwork for future research aimed at enhancing the meat quality of black goats.https://doi.org/10.1038/s41538-025-00476-x
spellingShingle Yong Long
Naifeng Zhang
Yanliang Bi
Tao Ma
Pramote Paengkoum
Wen Xiao
Yanpin Zhao
Chao Yuan
Defeng Wang
Yang Yang
Chaozhi Su
Yong Han
Integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds
npj Science of Food
title Integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds
title_full Integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds
title_fullStr Integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds
title_full_unstemmed Integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds
title_short Integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds
title_sort integrated transcriptomic analysis unveils molecular mechanisms regulating meat quality in newly improved black goat breeds
url https://doi.org/10.1038/s41538-025-00476-x
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