Optimizing the methodology for precise estimation of skeletal muscle fiber type proportions in humans

Optimizing the methodology for precise estimation of skeletal muscle fiber type proportions in humans

Abstract Isolating individual muscle fibers and characterizing their myosin heavy chain (MHC) content using SDS-PAGE has become an increasingly common method for describing skeletal muscle fiber type proportions. In this study, we aimed to assess how the number of muscle fibers analyzed, and whether...

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Bibliographic Details
Main Authors: Nathan Serrano, Matthew R. Buras, Lori R. Roust, Eleanna De Filippis, Christos S. Katsanos
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Subjects:
Muscle fibers
Muscle phenotype
Myosin heavy chain
Accuracy
Number of fibers
Selection of fibers
Online Access:https://doi.org/10.1038/s41598-025-15163-w
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https://doi.org/10.1038/s41598-025-15163-w

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