Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticide
Lambda-cyhalothrin (LC) is a pyrethroid insecticide employed to manage various arthropods as an alternative to persistent insecticides with minimized toxic effects on birds and mammals. However, LC causes oxidative and neurotoxic damages in fish Danio rerio. Effects of LC in morphology of liver of f...
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Elsevier
2025-06-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221475002500037X |
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| author | Tiago Venancio da Silva André Luiz do Nascimento Silva Karine Nathiele Nogueira Farias Carla Leticia Gediel Rivero Wendt Lucas de Oliveira Brasileiro Edilaine Fogaça Sabrina Fuzer Gonçalves Brenda de Oliveira Martins Maria Eduarda Corona Garcia Lilian Franco-Belussi Carlos Eurico S. Fernandes |
| author_facet | Tiago Venancio da Silva André Luiz do Nascimento Silva Karine Nathiele Nogueira Farias Carla Leticia Gediel Rivero Wendt Lucas de Oliveira Brasileiro Edilaine Fogaça Sabrina Fuzer Gonçalves Brenda de Oliveira Martins Maria Eduarda Corona Garcia Lilian Franco-Belussi Carlos Eurico S. Fernandes |
| author_sort | Tiago Venancio da Silva |
| collection | DOAJ |
| description | Lambda-cyhalothrin (LC) is a pyrethroid insecticide employed to manage various arthropods as an alternative to persistent insecticides with minimized toxic effects on birds and mammals. However, LC causes oxidative and neurotoxic damages in fish Danio rerio. Effects of LC in morphology of liver of fishes are scare. We aimed to establish the lethal concentration (LC50 96 h) of pyrethroid LC for Astianax lacustris. Then, we compare liver responses of sublethal doses of LC (i.e. 10.30 µg/L) for acute (i.e 1- and 3-days post-exposure - dpe) and chronic (i.e 6 and 12 dpe). We sought to identify pathological changes and compare liver histometric remodeling in fish subjected to acute and chronic toxicity tests. For this, liver histological changes were evaluated using the degree of tissue changes (DTC), followed by histomorphometric techniques determining structural volumetric density, glycogen cell density, and morphometry of hepatic tissue. We observed high hepatocellular injuries in exposed fishes. The main injuries included leukocyte infiltration, hyperemia, and pyknotic nuclei, especially 6 dpe. Hepatic glycogen storage decreases at 6 and 12 dpe showing metabolic damage. Both, density and volume of decreases after LC exposure in all exposure time. However, sinusoidal density increases after LC exposure, suggesting vascular hyperemia. These results show morphophysiological effects of LC at 10.30 µg/L in acute and chronic exposure represented by decreased glycogen storage, structural density, and volume of hepatocytes, in addition to a higher degree of histological changes. |
| format | Article |
| id | doaj-art-3dd73722bcc54d4eb03f1bb6a128e5a1 |
| institution | Kabale University |
| issn | 2214-7500 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj-art-3dd73722bcc54d4eb03f1bb6a128e5a12025-01-28T04:14:39ZengElsevierToxicology Reports2214-75002025-06-0114101919Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticideTiago Venancio da Silva0André Luiz do Nascimento Silva1Karine Nathiele Nogueira Farias2Carla Leticia Gediel Rivero Wendt3Lucas de Oliveira Brasileiro4Edilaine Fogaça5Sabrina Fuzer Gonçalves6Brenda de Oliveira Martins7Maria Eduarda Corona Garcia8Lilian Franco-Belussi9Carlos Eurico S. Fernandes10Universidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, Brazil; Programa de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, Brazil; Programa de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, Brazil; Programa de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, BrazilPrograma de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, BrazilPrograma de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, Brazil; Programa de pós graduação em Biologia Animal, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, Brazil; Programa de pós graduação em Biologia Animal, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, BrazilUniversidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Laboratório de Patologia Experimental - LAPEx, Instituto de Biociências – INBIO, Av. Costa e Silva, s/n, Universitá∼rio, Campo Grande, MS 79002-970, Brazil; Programa de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ, Universidade Federal de Mato Grosso do Sul, UFMS, Av. Costa e Silva, s/n, Universitário, Campo Grande, MS 79002-970, Brazil; Correspondence to: Laboratório de Patologia Experimental -LAPEx Instituto de Biociências - INBIO Universidade Federal de Mato Grosso do Sul – UFMS, Av. Costa e Silva, s/n, Bairro Universitário, Campo Grande, Mato Grosso do Sul 79002-970, BrazilLambda-cyhalothrin (LC) is a pyrethroid insecticide employed to manage various arthropods as an alternative to persistent insecticides with minimized toxic effects on birds and mammals. However, LC causes oxidative and neurotoxic damages in fish Danio rerio. Effects of LC in morphology of liver of fishes are scare. We aimed to establish the lethal concentration (LC50 96 h) of pyrethroid LC for Astianax lacustris. Then, we compare liver responses of sublethal doses of LC (i.e. 10.30 µg/L) for acute (i.e 1- and 3-days post-exposure - dpe) and chronic (i.e 6 and 12 dpe). We sought to identify pathological changes and compare liver histometric remodeling in fish subjected to acute and chronic toxicity tests. For this, liver histological changes were evaluated using the degree of tissue changes (DTC), followed by histomorphometric techniques determining structural volumetric density, glycogen cell density, and morphometry of hepatic tissue. We observed high hepatocellular injuries in exposed fishes. The main injuries included leukocyte infiltration, hyperemia, and pyknotic nuclei, especially 6 dpe. Hepatic glycogen storage decreases at 6 and 12 dpe showing metabolic damage. Both, density and volume of decreases after LC exposure in all exposure time. However, sinusoidal density increases after LC exposure, suggesting vascular hyperemia. These results show morphophysiological effects of LC at 10.30 µg/L in acute and chronic exposure represented by decreased glycogen storage, structural density, and volume of hepatocytes, in addition to a higher degree of histological changes.http://www.sciencedirect.com/science/article/pii/S221475002500037XHepatic tissueNeotropical fishPyrethroidToxicologyInsecticideHepatocytes |
| spellingShingle | Tiago Venancio da Silva André Luiz do Nascimento Silva Karine Nathiele Nogueira Farias Carla Leticia Gediel Rivero Wendt Lucas de Oliveira Brasileiro Edilaine Fogaça Sabrina Fuzer Gonçalves Brenda de Oliveira Martins Maria Eduarda Corona Garcia Lilian Franco-Belussi Carlos Eurico S. Fernandes Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticide Toxicology Reports Hepatic tissue Neotropical fish Pyrethroid Toxicology Insecticide Hepatocytes |
| title | Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticide |
| title_full | Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticide |
| title_fullStr | Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticide |
| title_full_unstemmed | Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticide |
| title_short | Liver morphometry and histopathology effects in Astyanax lacustris exposed to lambda-cyhalothrin pyrethroid insecticide |
| title_sort | liver morphometry and histopathology effects in astyanax lacustris exposed to lambda cyhalothrin pyrethroid insecticide |
| topic | Hepatic tissue Neotropical fish Pyrethroid Toxicology Insecticide Hepatocytes |
| url | http://www.sciencedirect.com/science/article/pii/S221475002500037X |
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