The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.)
Seed metabolomic profiles have been investigated in wild and cultivated forms (cultivars) of oat (Avena L.). Seed accessions from the VIR oat collection were used for the research. Metabolomic analysis employed gas liquid chromatography-mass spectrometry (GLC- MS) using an Agilent 6850 chromatograph...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2016-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/810 |
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| author | I. G. Loskutov T. V. Shelenga A. V. Konarev A. I. Shavarda E. V. Blinova N. I. Dzubenko |
| author_facet | I. G. Loskutov T. V. Shelenga A. V. Konarev A. I. Shavarda E. V. Blinova N. I. Dzubenko |
| author_sort | I. G. Loskutov |
| collection | DOAJ |
| description | Seed metabolomic profiles have been investigated in wild and cultivated forms (cultivars) of oat (Avena L.). Seed accessions from the VIR oat collection were used for the research. Metabolomic analysis employed gas liquid chromatography-mass spectrometry (GLC- MS) using an Agilent 6850 chromatographer (USA). The analysis covered the composition and content of organic and fatty acids, amino acids, polyatomic spirits and sugars. The content fluctuation range for the studied groups of compounds was found to be narrower (significantly in some cases) in cultivars than in the wild species. Along with a sharp increase in oleic acid content, cultivars demonstrated a decrease in that of linoleic acid. The general conclusions from the comparison of seed metabolomic profiles in wild species and cultivars are presented below. A number of wild species can be recommended as a potential source of biochemical quality traits for breeding purposes. A series of metabolites (compounds), the content of which changes during domestication or which differentiate wild oat species from cultivars has been identified was found. Along with such well-known healthy food chemical factors as oleic acid, glucose and fructose, etc., differences concerning monoacylglycerol compounds (MAG 16 : 0 and MAG-2 18 : 2, etc.) have been found. The latter have been proposed to be related to the formation of adaptive traits, in particular, resistance to diseases and pests, and to environmental abiotic stresses. |
| format | Article |
| id | doaj-art-3dc57f123dbc480fbdf635d69581bb15 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-3dc57f123dbc480fbdf635d69581bb152025-02-01T09:58:03ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592016-12-0120563664210.18699/VJ16.185525The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.)I. G. Loskutov0T. V. Shelenga1A. V. Konarev2A. I. Shavarda3E. V. Blinova4N. I. Dzubenko5The Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR) St.-Petersburg State UniversityThe Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR)The Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR)The Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR) St.-Petersburg State University V.L. Komarov Botanical Institute of the Russian Academy of SciencesThe Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR)The Federal Research Center All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR)Seed metabolomic profiles have been investigated in wild and cultivated forms (cultivars) of oat (Avena L.). Seed accessions from the VIR oat collection were used for the research. Metabolomic analysis employed gas liquid chromatography-mass spectrometry (GLC- MS) using an Agilent 6850 chromatographer (USA). The analysis covered the composition and content of organic and fatty acids, amino acids, polyatomic spirits and sugars. The content fluctuation range for the studied groups of compounds was found to be narrower (significantly in some cases) in cultivars than in the wild species. Along with a sharp increase in oleic acid content, cultivars demonstrated a decrease in that of linoleic acid. The general conclusions from the comparison of seed metabolomic profiles in wild species and cultivars are presented below. A number of wild species can be recommended as a potential source of biochemical quality traits for breeding purposes. A series of metabolites (compounds), the content of which changes during domestication or which differentiate wild oat species from cultivars has been identified was found. Along with such well-known healthy food chemical factors as oleic acid, glucose and fructose, etc., differences concerning monoacylglycerol compounds (MAG 16 : 0 and MAG-2 18 : 2, etc.) have been found. The latter have been proposed to be related to the formation of adaptive traits, in particular, resistance to diseases and pests, and to environmental abiotic stresses.https://vavilov.elpub.ru/jour/article/view/810avena l.wild speciesvarieties, seedsgas chromatographymass spectrometrymetabolomicsameliorationadaptability and polymorphism of characters. |
| spellingShingle | I. G. Loskutov T. V. Shelenga A. V. Konarev A. I. Shavarda E. V. Blinova N. I. Dzubenko The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.) Вавиловский журнал генетики и селекции avena l. wild species varieties, seeds gas chromatography mass spectrometry metabolomics amelioration adaptability and polymorphism of characters. |
| title | The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.) |
| title_full | The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.) |
| title_fullStr | The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.) |
| title_full_unstemmed | The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.) |
| title_short | The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.) |
| title_sort | metabolomic approach to the comparative analysis of wild and cultivated species of oats avena l |
| topic | avena l. wild species varieties, seeds gas chromatography mass spectrometry metabolomics amelioration adaptability and polymorphism of characters. |
| url | https://vavilov.elpub.ru/jour/article/view/810 |
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