Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels
Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial activity is associated with nitric oxide (NO) production. To understand the role of mitochondria in C...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Aging Research |
| Online Access: | http://dx.doi.org/10.4061/2011/673185 |
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| author | Bin Li Craig Skinner Pablo R. Castello Michiko Kato Erin Easlon Li Xie Tianlin Li Shu-Ping Lu Chen Wang Felicia Tsang Robert O. Poyton Su-Ju Lin |
| author_facet | Bin Li Craig Skinner Pablo R. Castello Michiko Kato Erin Easlon Li Xie Tianlin Li Shu-Ping Lu Chen Wang Felicia Tsang Robert O. Poyton Su-Ju Lin |
| author_sort | Bin Li |
| collection | DOAJ |
| description | Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial activity is associated with nitric oxide (NO) production. To understand the role of mitochondria in CR, we identify and study Saccharomyces cerevisiae mutants with increased NO levels as potential CR mimics. Analysis of the top 17 mutants demonstrates a correlation between increased NO, mitochondrial respiration, and longevity. Interestingly, treating yeast with NO donors such as GSNO (S-nitrosoglutathione) is sufficient to partially mimic CR to extend lifespan. CR-increased NO is largely dependent on mitochondrial electron transport and cytochrome c oxidase (COX). Although COX normally produces NO under hypoxic conditions, CR-treated yeast cells are able to produce NO under normoxic conditions. Our results suggest that CR may derepress some hypoxic genes for mitochondrial proteins that function to promote the production of NO and the extension of lifespan. |
| format | Article |
| id | doaj-art-3dbf67eb30674bb9a29e17462e713900 |
| institution | Kabale University |
| issn | 2090-2212 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Aging Research |
| spelling | doaj-art-3dbf67eb30674bb9a29e17462e7139002025-02-03T06:11:31ZengWileyJournal of Aging Research2090-22122011-01-01201110.4061/2011/673185673185Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide LevelsBin Li0Craig Skinner1Pablo R. Castello2Michiko Kato3Erin Easlon4Li Xie5Tianlin Li6Shu-Ping Lu7Chen Wang8Felicia Tsang9Robert O. Poyton10Su-Ju Lin11Department of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USAThe Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USAThe Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USADepartment of Microbiology, College of Biological Sciences, University of California at Davis, Davis, CA 95616, USACalorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial activity is associated with nitric oxide (NO) production. To understand the role of mitochondria in CR, we identify and study Saccharomyces cerevisiae mutants with increased NO levels as potential CR mimics. Analysis of the top 17 mutants demonstrates a correlation between increased NO, mitochondrial respiration, and longevity. Interestingly, treating yeast with NO donors such as GSNO (S-nitrosoglutathione) is sufficient to partially mimic CR to extend lifespan. CR-increased NO is largely dependent on mitochondrial electron transport and cytochrome c oxidase (COX). Although COX normally produces NO under hypoxic conditions, CR-treated yeast cells are able to produce NO under normoxic conditions. Our results suggest that CR may derepress some hypoxic genes for mitochondrial proteins that function to promote the production of NO and the extension of lifespan.http://dx.doi.org/10.4061/2011/673185 |
| spellingShingle | Bin Li Craig Skinner Pablo R. Castello Michiko Kato Erin Easlon Li Xie Tianlin Li Shu-Ping Lu Chen Wang Felicia Tsang Robert O. Poyton Su-Ju Lin Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels Journal of Aging Research |
| title | Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels |
| title_full | Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels |
| title_fullStr | Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels |
| title_full_unstemmed | Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels |
| title_short | Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels |
| title_sort | identification of potential calorie restriction mimicking yeast mutants with increased mitochondrial respiratory chain and nitric oxide levels |
| url | http://dx.doi.org/10.4061/2011/673185 |
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