Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid
BackgroundA novel fully automated chemiluminescent immunoassay (CLIA) has been developed to quantify autoantibodies specific for desmogleins (Dsg1, Dsg3), BP180, and BP230, key target antigens in pemphigus and bullous pemphigoid. This study aims to evaluate the diagnostic accuracy of the CLIA in dia...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1576635/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850098941329145856 |
|---|---|
| author | Na Zhang Ming Wang Lili Hou Yanyu Zhang Yan Zhao Luyang Du Xiaoqing Li Xia Liu Hongwei Zhou Jiaxuan Hao Jing Tian Qiuping Yu Lixia Li Fangming Cheng Hai-Zhou Zhou |
| author_facet | Na Zhang Ming Wang Lili Hou Yanyu Zhang Yan Zhao Luyang Du Xiaoqing Li Xia Liu Hongwei Zhou Jiaxuan Hao Jing Tian Qiuping Yu Lixia Li Fangming Cheng Hai-Zhou Zhou |
| author_sort | Na Zhang |
| collection | DOAJ |
| description | BackgroundA novel fully automated chemiluminescent immunoassay (CLIA) has been developed to quantify autoantibodies specific for desmogleins (Dsg1, Dsg3), BP180, and BP230, key target antigens in pemphigus and bullous pemphigoid. This study aims to evaluate the diagnostic accuracy of the CLIA in diagnosing pemphigus and BP and its correlation with disease severity and clinical features.MethodsSera from 106 bullous pemphigoid and 54 pemphigus patients and control sera from 153 patients with other skin disease and 121 healthy volunteers were included. CLIA and BIOCHIP mosaic-based indirect immunofluorescence (IIFT-BIOCHIP) were performed for all bullous pemphigoid and pemphigus patients, with ELISA conducted for most. Disease severity was assessed using the Body Surface Area scoring.ResultsThis CLIA showed strong agreement with IIFT-BIOCHIP, achieving area under the curve (AUC) values of 0.92 for anti-Dsg1/anti-Dsg3 and 0.84 for anti-BP180/anti-BP230 for differentiating pemphigus and BP. It outperformed ELISA (AUC: 0.73, 0.75) and was comparable to IIFT-BIOCHIP (AUC: 0.93, 0.87). The assay showed superior detection range and sensitivity compared to ELISA. Autoantibody levels correlated with disease severity and clinical symptoms, with elevated levels of anti-Dsg3 associated with mucosal lesions, anti-Dsg1/anti-Dsg3 associated with Nikolsky sign, and elevated anti-BP180/anti-BP230 levels linked to pruritus.ConclusionsThe novel CLIA, the first to quantify four major autoimmune blister disease autoantibodies (anti-Dsg1/anti-Dsg3/anti-BP180/anti-BP230) using a single sample tube, offers a simple and time-efficient test for diagnosing and screening pemphigus and BP. Its ability to assess disease severity and clinical relevance makes it a valuable tool for managing these conditions. |
| format | Article |
| id | doaj-art-3dbcc2591c8244eaa5c46519c3fc4a70 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-3dbcc2591c8244eaa5c46519c3fc4a702025-08-20T02:40:35ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.15766351576635Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoidNa Zhang0Ming Wang1Lili Hou2Yanyu Zhang3Yan Zhao4Luyang Du5Xiaoqing Li6Xia Liu7Hongwei Zhou8Jiaxuan Hao9Jing Tian10Qiuping Yu11Lixia Li12Fangming Cheng13Hai-Zhou Zhou14Department of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaGlobal Academic Department, Shenzhen YHLO Biotech Co., Ltd, Shenzhen, Guangdong, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaGlobal Academic Department, Shenzhen YHLO Biotech Co., Ltd, Shenzhen, Guangdong, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDermatology, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaGlobal Academic Department, Shenzhen YHLO Biotech Co., Ltd, Shenzhen, Guangdong, ChinaGlobal Academic Department, Shenzhen YHLO Biotech Co., Ltd, Shenzhen, Guangdong, ChinaReagent R&D Center, Shenzhen YHLO Biotech Co., Ltd, Shenzhen, Guangdong, ChinaDepartment of Laboratory Diagnostics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaBackgroundA novel fully automated chemiluminescent immunoassay (CLIA) has been developed to quantify autoantibodies specific for desmogleins (Dsg1, Dsg3), BP180, and BP230, key target antigens in pemphigus and bullous pemphigoid. This study aims to evaluate the diagnostic accuracy of the CLIA in diagnosing pemphigus and BP and its correlation with disease severity and clinical features.MethodsSera from 106 bullous pemphigoid and 54 pemphigus patients and control sera from 153 patients with other skin disease and 121 healthy volunteers were included. CLIA and BIOCHIP mosaic-based indirect immunofluorescence (IIFT-BIOCHIP) were performed for all bullous pemphigoid and pemphigus patients, with ELISA conducted for most. Disease severity was assessed using the Body Surface Area scoring.ResultsThis CLIA showed strong agreement with IIFT-BIOCHIP, achieving area under the curve (AUC) values of 0.92 for anti-Dsg1/anti-Dsg3 and 0.84 for anti-BP180/anti-BP230 for differentiating pemphigus and BP. It outperformed ELISA (AUC: 0.73, 0.75) and was comparable to IIFT-BIOCHIP (AUC: 0.93, 0.87). The assay showed superior detection range and sensitivity compared to ELISA. Autoantibody levels correlated with disease severity and clinical symptoms, with elevated levels of anti-Dsg3 associated with mucosal lesions, anti-Dsg1/anti-Dsg3 associated with Nikolsky sign, and elevated anti-BP180/anti-BP230 levels linked to pruritus.ConclusionsThe novel CLIA, the first to quantify four major autoimmune blister disease autoantibodies (anti-Dsg1/anti-Dsg3/anti-BP180/anti-BP230) using a single sample tube, offers a simple and time-efficient test for diagnosing and screening pemphigus and BP. Its ability to assess disease severity and clinical relevance makes it a valuable tool for managing these conditions.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1576635/fullpemphigusbullous pemphigoiddesmogleinBP180BP230chemiluminescent immunoassay |
| spellingShingle | Na Zhang Ming Wang Lili Hou Yanyu Zhang Yan Zhao Luyang Du Xiaoqing Li Xia Liu Hongwei Zhou Jiaxuan Hao Jing Tian Qiuping Yu Lixia Li Fangming Cheng Hai-Zhou Zhou Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid Frontiers in Immunology pemphigus bullous pemphigoid desmoglein BP180 BP230 chemiluminescent immunoassay |
| title | Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid |
| title_full | Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid |
| title_fullStr | Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid |
| title_full_unstemmed | Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid |
| title_short | Evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid |
| title_sort | evaluation of a novel chemiluminescent immunoassay for autoantibody detection in pemphigus and bullous pemphigoid |
| topic | pemphigus bullous pemphigoid desmoglein BP180 BP230 chemiluminescent immunoassay |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1576635/full |
| work_keys_str_mv | AT nazhang evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT mingwang evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT lilihou evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT yanyuzhang evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT yanzhao evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT luyangdu evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT xiaoqingli evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT xialiu evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT hongweizhou evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT jiaxuanhao evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT jingtian evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT qiupingyu evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT lixiali evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT fangmingcheng evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid AT haizhouzhou evaluationofanovelchemiluminescentimmunoassayforautoantibodydetectioninpemphigusandbullouspemphigoid |