Microplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural field

Abstract Microplastic ubiquity, its general toxicology, and its suitability for ingestion by biota are leading ecological and human health concerns. Microplastics are abundant in terrestrial environments including agricultural settings where municipal biosolids applied as fertilizers show high level...

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Main Authors: Emily R. McColville, Marshall W. Ritchie, Jesse Vermaire, David Lapen, Heath A. MacMillan, Jennifer F. Provencher, Jane E. Allison
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-06895-w
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author Emily R. McColville
Marshall W. Ritchie
Jesse Vermaire
David Lapen
Heath A. MacMillan
Jennifer F. Provencher
Jane E. Allison
author_facet Emily R. McColville
Marshall W. Ritchie
Jesse Vermaire
David Lapen
Heath A. MacMillan
Jennifer F. Provencher
Jane E. Allison
author_sort Emily R. McColville
collection DOAJ
description Abstract Microplastic ubiquity, its general toxicology, and its suitability for ingestion by biota are leading ecological and human health concerns. Microplastics are abundant in terrestrial environments including agricultural settings where municipal biosolids applied as fertilizers show high levels of microplastics. Microplastic ingestion by omnivorous insects in the environment is not well explored. To determine whether crickets eat microplastics in the wild, we examined the digestive tracts of 50 crickets collected from a research site in Ontario, Canada. Crickets were caught in three locations: a crop field amended with dewatered municipal biosolids, along the untreated edge of the field, and along a nearby tree line. Over half of the dissected crickets contained microplastics. A total of 87 microplastics (31–2548 μm) were found (60 microfragments; 27 microfibers). Using FTIR, we determined 66% of the microfragments were plastic polymers although match quality was low, likely due to exposure to multiple degradation processes (e.g. laundry, wastewater processing, ingestion by animals). Trap location did not influence the number of crickets ingesting microplastics. We present evidence that lab-reared crickets of the same species break down ingested microplastics into smaller fragments using manufactured polyethylene spheres, and discuss the possibility that generalist ground dwelling insects like crickets contribute to the active transport and biotransformation of microplastics, with potential cascading effects on microplastic movement through the food webs.
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spelling doaj-art-3db441fbdd1f468d815a589d4a0d80ef2025-08-20T03:03:42ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-06895-wMicroplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural fieldEmily R. McColville0Marshall W. Ritchie1Jesse Vermaire2David Lapen3Heath A. MacMillan4Jennifer F. Provencher5Jane E. Allison6Institute of Environmental Science, Carleton UniversityDepartment of Biology, Carleton UniversityInstitute of Environmental Science, Carleton UniversityOttawa Research Development Centre, Agriculture and Agri-Foods CanadaDepartment of Biology, Carleton UniversityEnvironment and Climate Change Canada, Carleton UniversityDepartment of Biology, Carleton UniversityAbstract Microplastic ubiquity, its general toxicology, and its suitability for ingestion by biota are leading ecological and human health concerns. Microplastics are abundant in terrestrial environments including agricultural settings where municipal biosolids applied as fertilizers show high levels of microplastics. Microplastic ingestion by omnivorous insects in the environment is not well explored. To determine whether crickets eat microplastics in the wild, we examined the digestive tracts of 50 crickets collected from a research site in Ontario, Canada. Crickets were caught in three locations: a crop field amended with dewatered municipal biosolids, along the untreated edge of the field, and along a nearby tree line. Over half of the dissected crickets contained microplastics. A total of 87 microplastics (31–2548 μm) were found (60 microfragments; 27 microfibers). Using FTIR, we determined 66% of the microfragments were plastic polymers although match quality was low, likely due to exposure to multiple degradation processes (e.g. laundry, wastewater processing, ingestion by animals). Trap location did not influence the number of crickets ingesting microplastics. We present evidence that lab-reared crickets of the same species break down ingested microplastics into smaller fragments using manufactured polyethylene spheres, and discuss the possibility that generalist ground dwelling insects like crickets contribute to the active transport and biotransformation of microplastics, with potential cascading effects on microplastic movement through the food webs.https://doi.org/10.1038/s41598-025-06895-wMicroplasticPollutionBiosolidSoilsEcotoxicologyInvertebrates
spellingShingle Emily R. McColville
Marshall W. Ritchie
Jesse Vermaire
David Lapen
Heath A. MacMillan
Jennifer F. Provencher
Jane E. Allison
Microplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural field
Scientific Reports
Microplastic
Pollution
Biosolid
Soils
Ecotoxicology
Invertebrates
title Microplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural field
title_full Microplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural field
title_fullStr Microplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural field
title_full_unstemmed Microplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural field
title_short Microplastics in the digestive tract of Gryllus pennsylvanicus crickets in a biosolid – treated agricultural field
title_sort microplastics in the digestive tract of gryllus pennsylvanicus crickets in a biosolid treated agricultural field
topic Microplastic
Pollution
Biosolid
Soils
Ecotoxicology
Invertebrates
url https://doi.org/10.1038/s41598-025-06895-w
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