Truncated gRNA reduces CRISPR/Cas9-mediated off-target rate for MSTN gene knockout in bovines

Truncated gRNA reduces CRISPR/Cas9-mediated off-target rate for MSTN gene knockout in bovines

The CRISPR/Cas9 mediates efficient gene editing but has off-target effects inconducive to animal breeding. In this study, the efficacy of CRISPR/Cas9 vectors containing different lengths of gRNA in reduction of the off-target phenomenon in the bovine MSTN gene knockout fibroblast cell lines was asse...

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Bibliographic Details
Main Authors: Zheng-wei ZHOU, Guo-hua CAO, Zhe LI, Xue-jie HAN, Chen LI, Zhen-yu LU, Yu-hang ZHAO, Xue-ling LI
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2019-12-01
Series:Journal of Integrative Agriculture
Subjects:
CRISPR/Cas9
gRNA
targeting site
off-target rate
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311919627449
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