A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage Form
A simple, rapid, and precise high-performance thin-layer chromatographic (HPTLC) method for simultaneous estimation of two antidiabetic drugs, metformin hydrochloride and sitagliptin phosphate, in tablet dosage form has been developed and validated. Chromatography was performed on silica gel 60 F254...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/139561 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849304730248937472 |
|---|---|
| author | Darshana K. Modi Punit B. Parejiya Bhavesh H. Patel |
| author_facet | Darshana K. Modi Punit B. Parejiya Bhavesh H. Patel |
| author_sort | Darshana K. Modi |
| collection | DOAJ |
| description | A simple, rapid, and precise high-performance thin-layer chromatographic (HPTLC) method for simultaneous estimation of two antidiabetic drugs, metformin hydrochloride and sitagliptin phosphate, in tablet dosage form has been developed and validated. Chromatography was performed on silica gel 60 F254 plates with butanol : water : glacial acetic acid (6 : 2 : 2, v/v/v) as mobile phase. This system gave a good resolution for metformin hydrochloride ( value of 0.35 ± 0.01) and sitagliptin phosphate ( value of 0.75 ± 0.01). Detection and quantification were carried out at 227 nm. The linear regression data for the calibration plot showed a good relationship with and 0.9991 for metformin hydrochloride and sitagliptin phosphate, respectively. The method was validated for precision and recovery. The limits of detection and quantification were 13.05 and 39.56 ng/μL for metformin hydrochloride and 2.65 and 8.03 ng/μL for sitagliptin phosphate, respectively. The amounts of the drugs in the marketed formulation were 99.86% and 98.91% for metformin hydrochloride and sitagliptin phosphate, respectively. |
| format | Article |
| id | doaj-art-3da91a1a8a39490280e616b29f7aa114 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-3da91a1a8a39490280e616b29f7aa1142025-08-20T03:55:40ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/139561139561A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage FormDarshana K. Modi0Punit B. Parejiya1Bhavesh H. Patel2Department of Pharmaceutical Chemistry, K. B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Gujarat, Gandhinagar 382023, IndiaDepartment of Pharmaceutical Chemistry, K. B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Gujarat, Gandhinagar 382023, IndiaDepartment of Pharmaceutical Chemistry, K. B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Gujarat, Gandhinagar 382023, IndiaA simple, rapid, and precise high-performance thin-layer chromatographic (HPTLC) method for simultaneous estimation of two antidiabetic drugs, metformin hydrochloride and sitagliptin phosphate, in tablet dosage form has been developed and validated. Chromatography was performed on silica gel 60 F254 plates with butanol : water : glacial acetic acid (6 : 2 : 2, v/v/v) as mobile phase. This system gave a good resolution for metformin hydrochloride ( value of 0.35 ± 0.01) and sitagliptin phosphate ( value of 0.75 ± 0.01). Detection and quantification were carried out at 227 nm. The linear regression data for the calibration plot showed a good relationship with and 0.9991 for metformin hydrochloride and sitagliptin phosphate, respectively. The method was validated for precision and recovery. The limits of detection and quantification were 13.05 and 39.56 ng/μL for metformin hydrochloride and 2.65 and 8.03 ng/μL for sitagliptin phosphate, respectively. The amounts of the drugs in the marketed formulation were 99.86% and 98.91% for metformin hydrochloride and sitagliptin phosphate, respectively.http://dx.doi.org/10.1155/2013/139561 |
| spellingShingle | Darshana K. Modi Punit B. Parejiya Bhavesh H. Patel A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage Form Journal of Chemistry |
| title | A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage Form |
| title_full | A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage Form |
| title_fullStr | A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage Form |
| title_full_unstemmed | A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage Form |
| title_short | A Simple and Sensitive HPTLC Method for Simultaneous Determination of Metformin Hydrochloride and Sitagliptin Phosphate in Tablet Dosage Form |
| title_sort | simple and sensitive hptlc method for simultaneous determination of metformin hydrochloride and sitagliptin phosphate in tablet dosage form |
| url | http://dx.doi.org/10.1155/2013/139561 |
| work_keys_str_mv | AT darshanakmodi asimpleandsensitivehptlcmethodforsimultaneousdeterminationofmetforminhydrochlorideandsitagliptinphosphateintabletdosageform AT punitbparejiya asimpleandsensitivehptlcmethodforsimultaneousdeterminationofmetforminhydrochlorideandsitagliptinphosphateintabletdosageform AT bhaveshhpatel asimpleandsensitivehptlcmethodforsimultaneousdeterminationofmetforminhydrochlorideandsitagliptinphosphateintabletdosageform AT darshanakmodi simpleandsensitivehptlcmethodforsimultaneousdeterminationofmetforminhydrochlorideandsitagliptinphosphateintabletdosageform AT punitbparejiya simpleandsensitivehptlcmethodforsimultaneousdeterminationofmetforminhydrochlorideandsitagliptinphosphateintabletdosageform AT bhaveshhpatel simpleandsensitivehptlcmethodforsimultaneousdeterminationofmetforminhydrochlorideandsitagliptinphosphateintabletdosageform |