Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing
CD133 mesenchymal cells were enriched using magnetic microbead anti-CD133 antibody from bone marrow mononuclear cells (BMMNCs). Flow cytometry and immunocytochemistry analysis using specific antibodies revealed that these cells were essentially 89 ± 4% CD133+ and 8 ± 5% CD34+. CD133+/CD34+ BMMNCs se...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2013/582527 |
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| author | Ubaidullo Kurbonov Abdusamad Dustov Alisher Barotov Murtazokul Khidirov Giesidin Mirojov Zikrie Rahimov Navjuvon Navjuvonov Eraj Rizoev Nasim Olimov Alijon Goibov Bakhtovar Karim-Zade Mukim Rakhmatov Suhayli Muminjonov Azadeh Didari Jamila Irgasheva Oktam Bobokhojaev Tashpulat Gulmuradov Amu Therwath Sohibnazar Rakhmonov Massoud Mirshahi |
| author_facet | Ubaidullo Kurbonov Abdusamad Dustov Alisher Barotov Murtazokul Khidirov Giesidin Mirojov Zikrie Rahimov Navjuvon Navjuvonov Eraj Rizoev Nasim Olimov Alijon Goibov Bakhtovar Karim-Zade Mukim Rakhmatov Suhayli Muminjonov Azadeh Didari Jamila Irgasheva Oktam Bobokhojaev Tashpulat Gulmuradov Amu Therwath Sohibnazar Rakhmonov Massoud Mirshahi |
| author_sort | Ubaidullo Kurbonov |
| collection | DOAJ |
| description | CD133 mesenchymal cells were enriched using magnetic microbead anti-CD133 antibody from bone marrow mononuclear cells (BMMNCs). Flow cytometry and immunocytochemistry analysis using specific antibodies revealed that these cells were essentially 89 ± 4% CD133+ and 8 ± 5% CD34+. CD133+/CD34+ BMMNCs secrete important bioactive proteins such as cardiotrophin-1, angiogenic and neurogenic factors, morphogenetic proteins, and proinflammatory and remodeling factors in vitro. Single intracoronary infusions of autologous CD133+/CD34+ BMMNCs are effective and reduce infarct size in patients as analyzed by Tc99m MIBI myocardial scintigraphy. The majority of patients were treated via left coronary artery. Nine months after cell therapy, 5 out of 8 patients showed a net positive response to therapy in different regions of the heart. Uptake of Tc99 isotope and revitalization of the heart area in inferoseptal region are more pronounced (P=0.016) as compared to apex and anterosptal regions after intracoronary injection of the stem cells. The cells chosen here have the properties essential for their potential use in cell therapy and their homing can be followed without major difficulty by the scintigraphy. The cell therapy proposed here is safe and should be practiced, as we found, in conjunction with scintigraphic observation of areas of heart which respond optimally to the infusion of autologous CD133+/CD34+ BMMNCs. |
| format | Article |
| id | doaj-art-3da8ca3c11484613a07ae6fcd0b4be57 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-3da8ca3c11484613a07ae6fcd0b4be572025-08-20T03:55:40ZengWileyStem Cells International1687-966X1687-96782013-01-01201310.1155/2013/582527582527Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell HomingUbaidullo Kurbonov0Abdusamad Dustov1Alisher Barotov2Murtazokul Khidirov3Giesidin Mirojov4Zikrie Rahimov5Navjuvon Navjuvonov6Eraj Rizoev7Nasim Olimov8Alijon Goibov9Bakhtovar Karim-Zade10Mukim Rakhmatov11Suhayli Muminjonov12Azadeh Didari13Jamila Irgasheva14Oktam Bobokhojaev15Tashpulat Gulmuradov16Amu Therwath17Sohibnazar Rakhmonov18Massoud Mirshahi19Avicenna Tajik State Medical University, Dushanbe, TajikistanInstitute of Gastroenterology, 734003 Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanAvicenna Tajik State Medical University, Dushanbe, TajikistanInstitute of Gastroenterology, 734003 Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanAvicenna Tajik State Medical University, Dushanbe, TajikistanAvicenna Tajik State Medical University, Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanAvicenna Tajik State Medical University, Dushanbe, TajikistanAvicenna Tajik State Medical University, Dushanbe, TajikistanAvicenna Tajik State Medical University, Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanTajikistan Ministry of Health, 734003 Dushanbe, TajikistanUMRS 872, CRC-INSERM, Université Pierre et Marie Curie, Paris-VI, Université Paris Descartes, Paris-V, 15 rue de l’Ecole de Médecine, 75006 Paris, FranceTajikistan Ministry of Health, 734003 Dushanbe, TajikistanAvicenna Tajik State Medical University, Dushanbe, TajikistanCD133 mesenchymal cells were enriched using magnetic microbead anti-CD133 antibody from bone marrow mononuclear cells (BMMNCs). Flow cytometry and immunocytochemistry analysis using specific antibodies revealed that these cells were essentially 89 ± 4% CD133+ and 8 ± 5% CD34+. CD133+/CD34+ BMMNCs secrete important bioactive proteins such as cardiotrophin-1, angiogenic and neurogenic factors, morphogenetic proteins, and proinflammatory and remodeling factors in vitro. Single intracoronary infusions of autologous CD133+/CD34+ BMMNCs are effective and reduce infarct size in patients as analyzed by Tc99m MIBI myocardial scintigraphy. The majority of patients were treated via left coronary artery. Nine months after cell therapy, 5 out of 8 patients showed a net positive response to therapy in different regions of the heart. Uptake of Tc99 isotope and revitalization of the heart area in inferoseptal region are more pronounced (P=0.016) as compared to apex and anterosptal regions after intracoronary injection of the stem cells. The cells chosen here have the properties essential for their potential use in cell therapy and their homing can be followed without major difficulty by the scintigraphy. The cell therapy proposed here is safe and should be practiced, as we found, in conjunction with scintigraphic observation of areas of heart which respond optimally to the infusion of autologous CD133+/CD34+ BMMNCs.http://dx.doi.org/10.1155/2013/582527 |
| spellingShingle | Ubaidullo Kurbonov Abdusamad Dustov Alisher Barotov Murtazokul Khidirov Giesidin Mirojov Zikrie Rahimov Navjuvon Navjuvonov Eraj Rizoev Nasim Olimov Alijon Goibov Bakhtovar Karim-Zade Mukim Rakhmatov Suhayli Muminjonov Azadeh Didari Jamila Irgasheva Oktam Bobokhojaev Tashpulat Gulmuradov Amu Therwath Sohibnazar Rakhmonov Massoud Mirshahi Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing Stem Cells International |
| title | Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing |
| title_full | Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing |
| title_fullStr | Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing |
| title_full_unstemmed | Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing |
| title_short | Intracoronary Infusion of Autologous CD133+ Cells in Myocardial Infarction and Tracing by Tc99m MIBI Scintigraphy of the Heart Areas Involved in Cell Homing |
| title_sort | intracoronary infusion of autologous cd133 cells in myocardial infarction and tracing by tc99m mibi scintigraphy of the heart areas involved in cell homing |
| url | http://dx.doi.org/10.1155/2013/582527 |
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