Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro study
Background and objectives. The endodontic treatment of primary teeth presents significant challenges due to their unique anatomical characteristics. To ensure a successful treatment, it is essential to evaluate a few factors. These factors include accurate diagnosis, disinfection protocol, intracana...
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| Language: | English |
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Amaltea Medical Publishing House
2024-06-01
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| Series: | Romanian Journal of Stomatology |
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| Online Access: | https://rjs.com.ro/articles/2024.2/RJS_2024_2_Art-03.pdf |
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| author | Divya Mukundan Maria Anthonet Sruthi |
| author_facet | Divya Mukundan Maria Anthonet Sruthi |
| author_sort | Divya Mukundan |
| collection | DOAJ |
| description | Background and objectives. The endodontic treatment of primary teeth presents significant challenges due to their unique anatomical characteristics. To ensure a successful treatment, it is essential to evaluate a few factors. These factors include accurate diagnosis, disinfection protocol, intracanal medicaments, and irrigation solutions. While Sodium hypochlorite (NaOCl) has gained success as an irrigation agent in primary teeth, it is important to note that higher concentrations of NaOCl can potentially cause toxicity to the periapical environment if they pass through the tooth's apical foramina. Thus, this study compared the cytotoxicity of two different concentrations of sodium hypochlorite at varied volumes on red blood cells (RBC). Materials and methods. In order to evaluate the cytotoxicity potential, fresh human blood was collected from a single healthy individual and subjected to testing. For the test, 10 µL, 20 µL, 30 µL, 40 µL and 50 µL of 1% and 3% NaOCl were added to the RBC. The tubes were gently mixed and incubated at 37°C for 30 minutes. A UV-visible spectrophotometer was used to calculate the absorbance of the supernatant at a wavelength of 540 nm. Results. There was no significant difference in 1% sodium hypochlorite at different volumes with a p value >0.05 using Tukey's post hoc test. However, there was a significant difference between the volumes of 3% sodium hypochlorite at 40 and 50 µg/ml with a p value of 0.004 and 0.001 respectively. Conclusions. According to the current study's findings, 1% sodium hypochlorite solution exhibited less toxicity than 3% sodium hypochlorite. |
| format | Article |
| id | doaj-art-3da798052e7a413989fb41751879ccec |
| institution | Kabale University |
| issn | 1843-0805 2069-6078 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Amaltea Medical Publishing House |
| record_format | Article |
| series | Romanian Journal of Stomatology |
| spelling | doaj-art-3da798052e7a413989fb41751879ccec2025-01-07T15:55:56ZengAmaltea Medical Publishing HouseRomanian Journal of Stomatology1843-08052069-60782024-06-0170213113610.37897/RJS.2024.2.3Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro studyDivya Mukundan0Maria Anthonet Sruthi1Department of Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaDepartment of Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaBackground and objectives. The endodontic treatment of primary teeth presents significant challenges due to their unique anatomical characteristics. To ensure a successful treatment, it is essential to evaluate a few factors. These factors include accurate diagnosis, disinfection protocol, intracanal medicaments, and irrigation solutions. While Sodium hypochlorite (NaOCl) has gained success as an irrigation agent in primary teeth, it is important to note that higher concentrations of NaOCl can potentially cause toxicity to the periapical environment if they pass through the tooth's apical foramina. Thus, this study compared the cytotoxicity of two different concentrations of sodium hypochlorite at varied volumes on red blood cells (RBC). Materials and methods. In order to evaluate the cytotoxicity potential, fresh human blood was collected from a single healthy individual and subjected to testing. For the test, 10 µL, 20 µL, 30 µL, 40 µL and 50 µL of 1% and 3% NaOCl were added to the RBC. The tubes were gently mixed and incubated at 37°C for 30 minutes. A UV-visible spectrophotometer was used to calculate the absorbance of the supernatant at a wavelength of 540 nm. Results. There was no significant difference in 1% sodium hypochlorite at different volumes with a p value >0.05 using Tukey's post hoc test. However, there was a significant difference between the volumes of 3% sodium hypochlorite at 40 and 50 µg/ml with a p value of 0.004 and 0.001 respectively. Conclusions. According to the current study's findings, 1% sodium hypochlorite solution exhibited less toxicity than 3% sodium hypochlorite.https://rjs.com.ro/articles/2024.2/RJS_2024_2_Art-03.pdfcytotoxicityprimary teethsodium hypochloritered blood cells |
| spellingShingle | Divya Mukundan Maria Anthonet Sruthi Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro study Romanian Journal of Stomatology cytotoxicity primary teeth sodium hypochlorite red blood cells |
| title | Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro study |
| title_full | Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro study |
| title_fullStr | Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro study |
| title_full_unstemmed | Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro study |
| title_short | Cytotoxic effect of 1% and 3% Sodium hypochlorite as irrigation agent in primary teeth on human red blood cells: an in vitro study |
| title_sort | cytotoxic effect of 1 and 3 sodium hypochlorite as irrigation agent in primary teeth on human red blood cells an in vitro study |
| topic | cytotoxicity primary teeth sodium hypochlorite red blood cells |
| url | https://rjs.com.ro/articles/2024.2/RJS_2024_2_Art-03.pdf |
| work_keys_str_mv | AT divyamukundan cytotoxiceffectof1and3sodiumhypochloriteasirrigationagentinprimaryteethonhumanredbloodcellsaninvitrostudy AT mariaanthonetsruthi cytotoxiceffectof1and3sodiumhypochloriteasirrigationagentinprimaryteethonhumanredbloodcellsaninvitrostudy |