Pathogenic differentiation and biological control of Verticillium wilt of eggplant
Verticillium wilt of eggplant occurs seriously in some areas of Zhejiang Province. To control the disease effectively, the disease observation and pathogenicity tests of pathogen were carried out, and the pathotype and physiological races of pathogen were identified. Furthermore, inhibitory activity...
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Zhejiang University Press
2019-08-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.11.262 |
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| author | LIU Jingjing PANG Yezhou ZHANG Jingze |
| author_facet | LIU Jingjing PANG Yezhou ZHANG Jingze |
| author_sort | LIU Jingjing |
| collection | DOAJ |
| description | Verticillium wilt of eggplant occurs seriously in some areas of Zhejiang Province. To control the disease effectively, the disease observation and pathogenicity tests of pathogen were carried out, and the pathotype and physiological races of pathogen were identified. Furthermore, inhibitory activity of Paenibacillus polymyxa strains against hypha growth and conidial germination of pathogen were determined, and inoculation assays for controlling Verticillium wilt of eggplant and plant growth promotion were conducted, and volatile metabolites and lipopeptides produced by P. polymyxa strains were analyzed. Results of disease observation and pathogenicity tests indicated that Verticillium wilt of eggplant in greenhouse occurred usually in mid-January, and it was caused by Verticillium dahliae Kleb. Identification results of pathotype and physiological races of pathogen with the representative strains demonstrated that seven isolates from Zhejiang Province all belonged to the defoliation pathotype and physiological race-2, respectively. In vitro experiments showed that the strain ShX301 had a highest inhibitory activity against hypha growth and conidial germination of pathogens isolated the diseased eggplant plants among five P. polymyxa strains. The in vivo experiments for controlling Verticillium wilt of eggplant and plant growth promotion displayed that the strain ShX301 had a highest inhibitory activity. Inoculation by the strain ShX301 reduced disease incidence and severity by 30% and 20.02%, respectively, and promoted eggplant plant growth with enhancing aboveground seedling stem length and biomass by 59.24% and 69.41%, as well as underground biomass by 54.11%, respectively. The determination of volatile metabolites showed that the five strains of P. polymyxa were able to produce volatile metabolites, which inhibited hypha growth of pathogen, but there were no significant differences among the strains. Matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis revealed that the fusaricidins were main activity compounds of antagonistic pathogen. Therefore, this study provides a scientific theory basis for development of the strain ShX301 as a biocontrol or plant growth promotion agent. |
| format | Article |
| id | doaj-art-3da32ee10ffc47c2b84969f598c6e157 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-3da32ee10ffc47c2b84969f598c6e1572025-08-20T03:58:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552019-08-014540741710.3785/j.issn.1008-9209.2018.11.26210089209Pathogenic differentiation and biological control of Verticillium wilt of eggplantLIU JingjingPANG YezhouZHANG JingzeVerticillium wilt of eggplant occurs seriously in some areas of Zhejiang Province. To control the disease effectively, the disease observation and pathogenicity tests of pathogen were carried out, and the pathotype and physiological races of pathogen were identified. Furthermore, inhibitory activity of Paenibacillus polymyxa strains against hypha growth and conidial germination of pathogen were determined, and inoculation assays for controlling Verticillium wilt of eggplant and plant growth promotion were conducted, and volatile metabolites and lipopeptides produced by P. polymyxa strains were analyzed. Results of disease observation and pathogenicity tests indicated that Verticillium wilt of eggplant in greenhouse occurred usually in mid-January, and it was caused by Verticillium dahliae Kleb. Identification results of pathotype and physiological races of pathogen with the representative strains demonstrated that seven isolates from Zhejiang Province all belonged to the defoliation pathotype and physiological race-2, respectively. In vitro experiments showed that the strain ShX301 had a highest inhibitory activity against hypha growth and conidial germination of pathogens isolated the diseased eggplant plants among five P. polymyxa strains. The in vivo experiments for controlling Verticillium wilt of eggplant and plant growth promotion displayed that the strain ShX301 had a highest inhibitory activity. Inoculation by the strain ShX301 reduced disease incidence and severity by 30% and 20.02%, respectively, and promoted eggplant plant growth with enhancing aboveground seedling stem length and biomass by 59.24% and 69.41%, as well as underground biomass by 54.11%, respectively. The determination of volatile metabolites showed that the five strains of P. polymyxa were able to produce volatile metabolites, which inhibited hypha growth of pathogen, but there were no significant differences among the strains. Matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis revealed that the fusaricidins were main activity compounds of antagonistic pathogen. Therefore, this study provides a scientific theory basis for development of the strain ShX301 as a biocontrol or plant growth promotion agent.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.11.262<italic>Paenibacillus polymyxa</italic>biological controlantagonistic mechanismvolatile compoundslipopeptides |
| spellingShingle | LIU Jingjing PANG Yezhou ZHANG Jingze Pathogenic differentiation and biological control of Verticillium wilt of eggplant 浙江大学学报. 农业与生命科学版 <italic>Paenibacillus polymyxa</italic> biological control antagonistic mechanism volatile compounds lipopeptides |
| title | Pathogenic differentiation and biological control of Verticillium wilt of eggplant |
| title_full | Pathogenic differentiation and biological control of Verticillium wilt of eggplant |
| title_fullStr | Pathogenic differentiation and biological control of Verticillium wilt of eggplant |
| title_full_unstemmed | Pathogenic differentiation and biological control of Verticillium wilt of eggplant |
| title_short | Pathogenic differentiation and biological control of Verticillium wilt of eggplant |
| title_sort | pathogenic differentiation and biological control of verticillium wilt of eggplant |
| topic | <italic>Paenibacillus polymyxa</italic> biological control antagonistic mechanism volatile compounds lipopeptides |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.11.262 |
| work_keys_str_mv | AT liujingjing pathogenicdifferentiationandbiologicalcontrolofverticilliumwiltofeggplant AT pangyezhou pathogenicdifferentiationandbiologicalcontrolofverticilliumwiltofeggplant AT zhangjingze pathogenicdifferentiationandbiologicalcontrolofverticilliumwiltofeggplant |