Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique
Bone-marrow-derived mesenchymal stem cells (MSCs) have the potential to differentiate into a number of phenotypes, including adipocytes. Adipogenic differentiation has traditionally been performed in monolayer culture, and, while the expression of a fat-cell phenotype can be achieved, this culture m...
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| Language: | English |
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Wiley
2013-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2013/806525 |
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| author | Jean F. Welter Kitsie J. Penick Luis A. Solchaga |
| author_facet | Jean F. Welter Kitsie J. Penick Luis A. Solchaga |
| author_sort | Jean F. Welter |
| collection | DOAJ |
| description | Bone-marrow-derived mesenchymal stem cells (MSCs) have the potential to differentiate into a number of phenotypes, including adipocytes. Adipogenic differentiation has traditionally been performed in monolayer culture, and, while the expression of a fat-cell phenotype can be achieved, this culture method is labor and material intensive and results in only small numbers of fragile adherent cells, which are not very useful for further applications. Aggregate culture is a cell-culture technique in which cells are induced to form three-dimensional aggregates; this method has previously been used successfully, among others, to induce and study chondrogenic differentiation of MSCs. We have previously published an adaptation of the chondrogenic aggregate culture method to a 96-well plate format. Based on the success of this method, we have used the same format for the preparation of three-dimensional adipogenic cultures. The MSCs differentiate rapidly, the aggregates can be handled and processed for histologic and biochemical assays with ease, and the format offers significant savings in supplies and labor. As a differentiation assay, this method can distinguish between degrees of senescence and appears suitable for testing medium or drug formulations in a high-volume, high-throughput fashion. |
| format | Article |
| id | doaj-art-3d99caa14b294be7b1501e7345a7b85f |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-3d99caa14b294be7b1501e7345a7b85f2025-08-20T02:08:20ZengWileyStem Cells International1687-966X1687-96782013-01-01201310.1155/2013/806525806525Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening TechniqueJean F. Welter0Kitsie J. Penick1Luis A. Solchaga2Department of Biology, Skeletal Research Center, Case Western Reserve University, 2080 Adelbert Road, Millis Science Center, Room 112A, 2080 Adelbert Rood, Cleveland, OH 44106-7080, USADepartment of Biology, Skeletal Research Center, Case Western Reserve University, 2080 Adelbert Road, Millis Science Center, Room 112A, 2080 Adelbert Rood, Cleveland, OH 44106-7080, USACase Comprehensive Cancer Center, Department of General Medical Sciences and Division of Hematology and Oncology, Case Western Reserve University, Cleveland, OH, USABone-marrow-derived mesenchymal stem cells (MSCs) have the potential to differentiate into a number of phenotypes, including adipocytes. Adipogenic differentiation has traditionally been performed in monolayer culture, and, while the expression of a fat-cell phenotype can be achieved, this culture method is labor and material intensive and results in only small numbers of fragile adherent cells, which are not very useful for further applications. Aggregate culture is a cell-culture technique in which cells are induced to form three-dimensional aggregates; this method has previously been used successfully, among others, to induce and study chondrogenic differentiation of MSCs. We have previously published an adaptation of the chondrogenic aggregate culture method to a 96-well plate format. Based on the success of this method, we have used the same format for the preparation of three-dimensional adipogenic cultures. The MSCs differentiate rapidly, the aggregates can be handled and processed for histologic and biochemical assays with ease, and the format offers significant savings in supplies and labor. As a differentiation assay, this method can distinguish between degrees of senescence and appears suitable for testing medium or drug formulations in a high-volume, high-throughput fashion.http://dx.doi.org/10.1155/2013/806525 |
| spellingShingle | Jean F. Welter Kitsie J. Penick Luis A. Solchaga Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique Stem Cells International |
| title | Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique |
| title_full | Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique |
| title_fullStr | Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique |
| title_full_unstemmed | Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique |
| title_short | Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three-Dimensional Culture Screening Technique |
| title_sort | assessing adipogenic potential of mesenchymal stem cells a rapid three dimensional culture screening technique |
| url | http://dx.doi.org/10.1155/2013/806525 |
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